CENPA (Ab-7) Antibody (PACO60632)
- SKU:
- PACO60632
- Product type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Application:
- ELISA
- Application:
- IHC
- Antibody type:
- Polyclonal
- Conjugation:
- Unconjugated
Frequently bought together:
Description
抗体名: | CENPA (Ab-7) Antibody (PACO60632) |
抗体コード: | PACO60632 |
サイズ: | 50ul |
宿主種: | Rabbit |
申し込み: | ELISA, IHC |
推奨される希釈: | ELISA:1:2000-1:10000, IHC:1:10-1:100 |
反応性: | Human |
免疫原: | Peptide sequence around site of Ser (7) derived from Human Histone H3-like centromeric protein A |
憲法: | Liquid |
ストレージバッファ: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
精製方法: | Antigen Affinity Purified |
抗体のクローン性: | Polyclonal |
アイソタイプ: | IgG |
Conjugate: | Non-conjugated |
IHC image of PACO60632 diluted at 1:10 and staining in paraffin-embedded human liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. | |
IHC image of PACO60632 diluted at 1:10 and staining in paraffin-embedded human cervical cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
バックグラウンド: | Histone H3-like nucleosomal protein that is specifically found in centromeric nucleosomes. Replaces conventional H3 in the nucleosome core of centromeric chromatin at the inner plate of the kinetochore. The presence of CENPA subtly modifies the nucleosome structure and the way DNA is wrapped around the nucleosome and gives rise to protruding DNA ends that are less well-ordered and rigid compared to nucleosomes containing histone H3. May serve as an epigenetic mark that propagates centromere identity through replication and cell division. Required for recruitment and assembly of kinetochore proteins, and as a consequence required for progress through mitosis, chromosome segregation and cytokinesis. |
シノニム: | Histone H3-like centromeric protein A (Centromere autoantigen A) (Centromere protein A) (CENP-A), CENPA |
UniProt Protein Function: | CENPA: a basic nuclear protein of the histone H3 family. May act as a core histone necessary for the assembly of centromeres. Contains a histone H3 related histone fold domain that is required for targeting to the centromere. CENPA is proposed to be a component of a modified nucleosome or nucleosome-like structure in which it replaces 1 or both copies of conventional histone H3 in the (H3-H4)2 tetrameric core of the nucleosome particle. |
UniProt Protein Details: | Protein type:DNA-binding Chromosomal Location of Human Ortholog: 2p23.3 Cellular Component: chromosome, pericentric region; condensed nuclear chromosome, pericentric region; cytosol; nucleoplasm; nucleus Molecular Function:chromatin binding; protein binding Biological Process: DNA replication-independent nucleosome assembly at centromere; establishment of mitotic spindle orientation; kinetochore assembly; sister chromatid cohesion |
NCBI Summary: | Centromeres are the differentiated chromosomal domains that specify the mitotic behavior of chromosomes. This gene encodes a centromere protein which contains a histone H3 related histone fold domain that is required for targeting to the centromere. Centromere protein A is proposed to be a component of a modified nucleosome or nucleosome-like structure in which it replaces 1 or both copies of conventional histone H3 in the (H3-H4)2 tetrameric core of the nucleosome particle. The protein is a replication-independent histone that is a member of the histone H3 family. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Nov 2015] |
UniProt Code: | P49450 |
NCBI GenInfo Identifier: | 1345726 |
NCBI Gene ID: | 1058 |
NCBI Accession: | P49450.1 |
UniProt Secondary Accession: | P49450,Q53T74, Q9BVW2, D6W544, |
UniProt Related Accession: | P49450 |
Molecular Weight: | 13,001 Da |
NCBI Full Name: | Histone H3-like centromeric protein A |
NCBI Synonym Full Names: | centromere protein A |
NCBI Official Symbol: | CENPA |
NCBI Official Synonym Symbols: | CenH3; CENP-A |
NCBI Protein Information: | histone H3-like centromeric protein A |
UniProt Protein Name: | Histone H3-like centromeric protein A |
UniProt Synonym Protein Names: | Centromere autoantigen A; Centromere protein A; CENP-A |
Protein Family: | Histone H3-like centromeric protein |
UniProt Gene Name: | CENPA |
UniProt Entry Name: | CENPA_HUMAN |
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