Anti-Protein Wnt-3a Monoclonal Antibody (CAB9923)
- SKU:
- CAB9923
- Product type:
- Antibody
- Application:
- WB
- Reactivity:
- Human
- Host Species:
- Mouse
- Isotype:
- IgG
- Research Area:
- Metabolism
Description
| 抗体名: | Anti-Protein Wnt-3a Monoclonal Antibody |
| 抗体コード: | CAB9923 |
| 抗体サイズ: | 100µL |
| 申し込み: | WB |
| 反応性: | Human |
| 宿主種: | Mouse |
| 免疫原: | Recombinant protein of human WNT3A |
| 申し込み: | WB |
| 推奨希釈: | WB 1:500 - 1:2000 |
| 反応性: | Human |
| ポジティブサンプル: | HepG2 |
| 免疫原: | Recombinant protein of human WNT3A |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | Email for sequence |
| 遺伝子ID: | 89780 |
| Uniprot: | P56704 |
| セルラーロケーション: | Secreted, extracellular matrix, extracellular space |
| 計算された分子量: | 39kDa/42kDa |
| 観察された分子量: | 42kDa |
| 同義語: | WNT3A |
| バックグラウンド: | The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. It encodes a protein which shows 96% amino acid identity to mouse Wnt3A protein, and 84% to human WNT3 protein, another WNT gene product. This gene is clustered with WNT14 gene, another family member, in chromosome 1q42 region. |
| UniProt Protein Function: | WNT3A: Ligand for members of the frizzled family of seven transmembrane receptors. Wnt-3 and Wnt-3a play distinct roles in cell-cell signaling during morphogenesis of the developing neural tube. Interacts with PORCN. Interacts with APCDD1 and WLS. Component of the Wnt-Fzd-LRP5-LRP6 signaling complex that contains a WNT protein, a FZD protein and LRP5 or LRP6. Interacts directly in the complex with LRP6. Moderately expressed in placenta and at low levels in adult lung, spleen, and prostate. Belongs to the Wnt family. |
| UniProt Protein Details: | Protein type:Secreted; Secreted, signal peptide Chromosomal Location of Human Ortholog: 1q42 Cellular Component: proteinaceous extracellular matrix; extracellular space; cell surface; endoplasmic reticulum lumen; early endosome membrane; Golgi lumen; plasma membrane; extracellular region Molecular Function:protein domain specific binding; protein binding; frizzled binding; transcription coactivator activity; receptor agonist activity; frizzled-2 binding Biological Process: positive regulation of mesodermal cell fate specification; axon guidance; extracellular matrix organization and biogenesis; positive regulation of protein binding; positive regulation of transcription, DNA-dependent; cell proliferation in forebrain; positive regulation of receptor internalization; positive regulation of caspase activity; Wnt receptor signaling pathway through beta-catenin; positive regulation of collateral sprouting in the absence of injury; palate development; negative regulation of axon extension involved in axon guidance; Wnt receptor signaling pathway in forebrain neuroblast division; negative regulation of neurogenesis; neuron differentiation; mammary gland development; positive regulation of cell proliferation; positive regulation of B cell proliferation; hemopoiesis; heart looping; dorsoventral neural tube patterning; inner ear morphogenesis; in utero embryonic development; hippocampus development; negative regulation of fat cell differentiation; positive regulation of peptidyl-serine phosphorylation; osteoblast differentiation; paraxial mesodermal cell fate commitment; signalosome assembly; spinal cord association neuron differentiation; positive regulation of transcription factor activity; positive regulation of transcription from RNA polymerase II promoter; positive regulation of protein amino acid phosphorylation |
| NCBI Summary: | The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. It encodes a protein which shows 96% amino acid identity to mouse Wnt3A protein, and 84% to human WNT3 protein, another WNT gene product. This gene is clustered with WNT14 gene, another family member, in chromosome 1q42 region. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P56704 |
| NCBI GenInfo Identifier: | 20532424 |
| NCBI Gene ID: | 89780 |
| NCBI Accession: | P56704.2 |
| UniProt Secondary Accession: | P56704,Q3SY79, Q3SY80, Q969P2, |
| UniProt Related Accession: | P56704 |
| Molecular Weight: | 39kDa |
| NCBI Full Name: | Protein Wnt-3a |
| NCBI Synonym Full Names: | wingless-type MMTV integration site family, member 3A |
| NCBI Official Symbol: | WNT3A |
| NCBI Protein Information: | protein Wnt-3a |
| UniProt Protein Name: | Protein Wnt-3a |
| UniProt Gene Name: | WNT3A |
| UniProt Entry Name: | WNT3A_HUMAN |
 | Western blot analysis of extracts of HepG2 cells, using WNT3A antibody (CAB9923) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) (CABS003) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 90s. |
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