Anti-ACLY 抗体 (CAB9901)

抗体名:	Anti-ACLY Monoclonal Antibody
抗体コード:	CAB9901
抗体サイズ:	100µL
申し込み:	WB ICC FC
反応性:	Human, Mouse, Monkey
宿主種:	Mouse
免疫原:	Recombinant protein of human ACLY

申し込み:	WB ICC FC
推奨希釈:	WB 1:500 - 1:2000 ICC 1:50 - 1:200 FC 1:20 - 1:50
反応性:	Human, Mouse, Monkey
ポジティブサンプル:	HeLa, K-562, COS-7, HepG2, Raji, NIH/3T3, Mouse kidney

免疫原:	Recombinant protein of human ACLY
精製方法:	Affinity purification
ストレージバッファ:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
アイソタイプ:	IgG
順序:	Email for sequence
遺伝子ID:	47
Uniprot:	P53396
セルラーロケーション:	Cytoplasm
計算された分子量:	91kDa/119kDa/120kDa
観察された分子量:	121kDa

同義語:

ACLY, ACL, ATPCL, CLATP

バックグラウンド:

ATP citrate lyase is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many tissues. The enzyme is a tetramer (relative molecular weight approximately 440, 000) of apparently identical subunits. It catalyzes the formation of acetyl-CoA and oxaloacetate from citrate and CoA with a concomitant hydrolysis of ATP to ADP and phosphate. The product, acetyl-CoA, serves several important biosynthetic pathways, including lipogenesis and cholesterogenesis. In nervous tissue, ATP citrate-lyase may be involved in the biosynthesis of acetylcholine. Multiple transcript variants encoding distinct isoforms have been identified for this gene.

UniProt Protein Function:	ACLY: ATP citrate-lyase is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many tissues. Has a central role in de novo lipid synthesis. In nervous tissue it may be involved in the biosynthesis of acetylcholine. Homotetramer. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:EC 2.3.3.8; Transferase; Lyase; Carbohydrate Metabolism - citrate (TCA) cycle Chromosomal Location of Human Ortholog: 17q21.2 Cellular Component: cytoplasm; cytosol; membrane; mitochondrion; nucleoplasm; plasma membrane Molecular Function:ATP binding; ATP citrate synthase activity; cofactor binding; metal ion binding; protein binding Biological Process: acetyl-CoA biosynthetic process; cellular lipid metabolic process; cholesterol biosynthetic process; citrate metabolic process; energy reserve metabolic process; fatty acid biosynthetic process; lipid biosynthetic process; oxaloacetate metabolic process; positive regulation of cellular metabolic process; triacylglycerol biosynthetic process
NCBI Summary:	ATP citrate lyase is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many tissues. The enzyme is a tetramer (relative molecular weight approximately 440,000) of apparently identical subunits. It catalyzes the formation of acetyl-CoA and oxaloacetate from citrate and CoA with a concomitant hydrolysis of ATP to ADP and phosphate. The product, acetyl-CoA, serves several important biosynthetic pathways, including lipogenesis and cholesterogenesis. In nervous tissue, ATP citrate-lyase may be involved in the biosynthesis of acetylcholine. Multiple transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Dec 2014]
UniProt Code:	P53396
NCBI GenInfo Identifier:	116241237
NCBI Gene ID:	47
NCBI Accession:	P53396.3
UniProt Secondary Accession:	P53396,Q13037, Q9BRL0, B4DIM0, B4E3P0,
UniProt Related Accession:	P53396
Molecular Weight:	91,099 Da
NCBI Full Name:	ATP-citrate synthase
NCBI Synonym Full Names:	ATP citrate lyase
NCBI Official Symbol:	ACLY
NCBI Official Synonym Symbols:	ACL; ATPCL; CLATP
NCBI Protein Information:	ATP-citrate synthase
UniProt Protein Name:	ATP-citrate synthase
UniProt Synonym Protein Names:	ATP-citrate (pro-S-)-lyase; ACL; Citrate cleavage enzyme
Protein Family:	ATP-citrate synthase
UniProt Gene Name:	ACLY
UniProt Entry Name:	ACLY_HUMAN

Western blot analysis of extracts of various cell lines, using ACLY antibody (CAB9901) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) (CABS003) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.

Antibodies	ELISA Kits
Anti-ACLY Antibody (CAB15251)	Human ATP citrate lyase / ACLY ELISA Kit
Anti-Phospho-ACLY-S455 pAb Antibody (CABP0779)	Human ATP-citrate synthase (ACLY) ELISA Kit
Anti-ACLY Antibody (CAB3719)	ATP-Citrate Lyase Colorimetric Cell-Based ELISA Kit
Phospho-ACLY (S455) Antibody (PACO04404)	ATP-Citrate Lyase (Phospho-Ser454) Colorimetric Cell-Based ELISA Kit
ACLY Antibody (PACO06271)

Secondary Antibody

Anti-HRP Goat Anti-Mouse IgG (H+L) Antibody (CABS003)