SRGAP1 Antibody (PACO20600)
- SKU:
- PACO20600
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Application:
- ELISA
- Application:
- WB
- Application:
- IHC
- Antibody type:
- Polyclonal
- Conjugation:
- Unconjugated
Frequently bought together:
Description
抗体名: | SRGAP1 Antibody (PACO20600) |
抗体コード: | PACO20600 |
サイズ: | 50ul |
宿主種: | Rabbit |
申し込み: | ELISA, WB, IHC |
推奨される希釈: | ELISA:1:2000-1:5000, WB:1:500-1:2000, IHC:1:25-1:100 |
反応性: | Human, Mouse |
免疫原: | Synthetic peptide of human SRGAP1 |
憲法: | Liquid |
ストレージバッファ: | -20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol |
精製方法: | Antigen affinity purification |
抗体のクローン性: | Polyclonal |
アイソタイプ: | IgG |
Conjugate: | Non-conjugated |
The image on the left is immunohistochemistry of paraffin-embedded Human breast cancer tissue using PACO20600(SRGAP1 Antibody) at dilution 1/25, on the right is treated with synthetic peptide. (Original magnification: x200). | |
Gel: 6%SDS-PAGE, Lysate: 40 µg, Lane 1-4: Hepg2 cells, TM4 cells, 293T cells, NIH/3T3 cells, Primary antibody: PACO20600(SRGAP1 Antibody) at dilution 1/200, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 20 seconds. | |
The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using PACO20600(SRGAP1 Antibody) at dilution 1/25, on the right is treated with synthetic peptide. (Original magnification: x200). |
バックグラウンド: | SRGAP1 contains an FCH (Fes/CIP4 homology) domain, a Rho-GAP domain and an SH3 domain. In the presence of Slit, SRGAP1 (via its SH3 domain) binds to the CC3 motif in robo (a protein responsible for mediating the repulsive effect of Slit) with higher affinity and inhibits Cdc42 activity in a robo/SRGAP-dependent manner. More specifically, SRGAP1 increases the intrinsic GTPase activity of Cdc42, thereby converting it to its inactive, GDP-bound form. Inactivation of Cdc42 ultimately leads to a decrease in actin polymerization. |
シノニム: | SLIT-ROBO Rho GTPase activating protein 1 |
UniProt Protein Function: | ARHGAP13: GTPase-activating protein for RhoA and Cdc42 small GTPases. Together with CDC42 seems to be involved in the pathway mediating the repulsive signaling of Robo and Slit proteins in neuronal migration. SLIT2, probably through interaction with ROBO1, increases the interaction of SRGAP1 with ROBO1 and inactivates CDC42. 2 isoforms of the human protein are produced by alternative splicing. |
UniProt Protein Details: | Protein type:GAPs; GAPs, Rac/Rho Chromosomal Location of Human Ortholog: 12q14.2 Cellular Component: cytoplasm; cytosol Molecular Function:GTPase activator activity; protein binding; Rac GTPase binding Biological Process: negative regulation of cell migration; regulation of small GTPase mediated signal transduction Disease: Thyroid Carcinoma, Follicular |
UniProt Code: | Q7Z6B7 |
NCBI GenInfo Identifier: | 48428624 |
NCBI Gene ID: | 57522 |
NCBI Accession: | Q7Z6B7.1 |
UniProt Secondary Accession: | Q7Z6B7,Q9H8A3, Q9P2P2, |
UniProt Related Accession: | Q7Z6B7 |
Molecular Weight: | 121,594 Da |
NCBI Full Name: | SLIT-ROBO Rho GTPase-activating protein 1 |
NCBI Synonym Full Names: | SLIT-ROBO Rho GTPase activating protein 1 |
NCBI Official Symbol: | SRGAP1 |
NCBI Official Synonym Symbols: | NMTC2; ARHGAP13 |
NCBI Protein Information: | SLIT-ROBO Rho GTPase-activating protein 1 |
UniProt Protein Name: | SLIT-ROBO Rho GTPase-activating protein 1 |
UniProt Synonym Protein Names: | Rho GTPase-activating protein 13 |
Protein Family: | SLIT-ROBO Rho GTPase-activating protein |
UniProt Gene Name: | SRGAP1 |
UniProt Entry Name: | SRGP1_HUMAN |