SEMA4D Antibody (PACO59269)
- SKU:
- PACO59269
- Product type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Application:
- ELISA
- Application:
- IF
- Antibody type:
- Polyclonal
- Conjugation:
- Unconjugated
Description
抗体名: | SEMA4D Antibody (PACO59269) |
抗体コード: | PACO59269 |
サイズ: | 50ug |
宿主種: | Rabbit |
申し込み: | ELISA, IF |
推奨される希釈: | ELISA:1:2000-1:10000, IF:1:50-1:200 |
反応性: | Human |
免疫原: | Recombinant Human Semaphorin-4D protein (756-862AA) |
憲法: | Liquid |
ストレージバッファ: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
精製方法: | >95%, Protein G purified |
抗体のクローン性: | Polyclonal |
アイソタイプ: | IgG |
Conjugate: | Non-conjugated |
Immunofluorescence staining of MCF-7 cells with PACO59269 at 1:166, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). |
バックグラウンド: | Cell surface receptor for PLXN1B and PLXNB2 that plays an important role in cell-cell signaling. Promotes reorganization of the actin cytoskeleton and plays a role in axonal growth cone guidance in the developing central nervous system. Regulates dendrite and axon branching and morphogenesis. Promotes the migration of cerebellar granule cells and of endothelial cells. Plays a role in the immune system; induces B-cells to aggregate and improves their viability (in vitro). Promotes signaling via SRC and PTK2B/PYK2, which then mediates activation of phosphatidylinositol 3-kinase and of the AKT1 signaling cascade. Interaction with PLXNB1 mediates activation of RHOA. |
シノニム: | Semaphorin-4D (A8) (BB18) (GR3) (CD antigen CD100), SEMA4D, C9orf164 CD100 SEMAJ |
UniProt Protein Function: | SEMA4D: a single-pass type I membrane protein that my play a functional role in the immune system, as well as in the nervous system. Induces B-cells to aggregate and improves their viability in vitro. |
UniProt Protein Details: | Protein type:Membrane protein, integral Chromosomal Location of Human Ortholog: 9q22.2 Cellular Component: extracellular space; integral to plasma membrane; plasma membrane Molecular Function:chemorepellent activity; protein binding; receptor activity; receptor binding; semaphorin receptor binding; transmembrane receptor activity Biological Process: axon guidance; cell adhesion; immune response; negative chemotaxis; negative regulation of apoptosis; negative regulation of axon extension involved in axon guidance; negative regulation of cell adhesion; negative regulation of osteoblast differentiation; negative regulation of peptidyl-tyrosine phosphorylation; negative regulation of transcription from RNA polymerase II promoter; neural crest cell migration; positive regulation of cell migration; positive regulation of collateral sprouting; positive regulation of GTPase activity; positive regulation of peptidyl-tyrosine phosphorylation; positive regulation of phosphoinositide 3-kinase cascade; positive regulation of protein amino acid phosphorylation; regulation of cell projection organization and biogenesis; regulation of cell shape; regulation of dendrite morphogenesis |
UniProt Code: | Q92854 |
NCBI GenInfo Identifier: | 8134701 |
NCBI Gene ID: | 10507 |
NCBI Accession: | Q92854.1 |
UniProt Secondary Accession: | Q92854,Q7Z5S4, Q8N8B0, B2RPM6, |
UniProt Related Accession: | Q92854 |
Molecular Weight: | 82,164 Da |
NCBI Full Name: | Semaphorin-4D |
NCBI Synonym Full Names: | semaphorin 4D |
NCBI Official Symbol: | SEMA4D |
NCBI Official Synonym Symbols: | CD100; SEMAJ; coll-4; C9orf164; M-sema-G |
NCBI Protein Information: | semaphorin-4D |
UniProt Protein Name: | Semaphorin-4D |
UniProt Synonym Protein Names: | A8; BB18; GR3; CD_antigen: CD100 |
Protein Family: | Semaphorin |
UniProt Gene Name: | SEMA4D |
UniProt Entry Name: | SEM4D_HUMAN |
Antibodies | ELISA Kits |
Anti-SEMA4D Antibody (CAB10136) | Human Semaphorin 4D / SEMA4D ELISA Kit |
SEMA4D Antibody (PACO01964) | Human Semaphorin-4D (SEMA4D) ELISA Kit |
Secondary Antibody |
Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014) |
Recommended Products |
Anti-FITC Goat Anti-Rabbit IgG (H+L) Antibody (CABS011) |
Anti-HRP-conjugated Beta Actin Antibody (CABC028) |