RPS6KA5 Antibody (PACO49990)
- SKU:
- PACO49990
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Application:
- ELISA
- Application:
- WB
- Application:
- IHC
- Application:
- IF
- Antibody type:
- Polyclonal
- Conjugation:
- Unconjugated
Description
抗体名: | RPS6KA5 Antibody (PACO49990) |
抗体コード: | PACO49990 |
サイズ: | 50ug |
宿主種: | Rabbit |
申し込み: | ELISA, WB, IHC, IF |
推奨される希釈: | ELISA:1:2000-1:10000, WB:1:500-1:5000, IHC:1:20-1:200, IF:1:50-1:200 |
反応性: | Human, Mouse, Rat |
免疫原: | Recombinant Human Ribosomal protein S6 kinase alpha-5 protein (224-367AA) |
憲法: | Liquid |
ストレージバッファ: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
精製方法: | >95%, Protein G purified |
抗体のクローン性: | Polyclonal |
アイソタイプ: | IgG |
Conjugate: | Non-conjugated |
Western Blot. Positive WB detected in: Mouse brain tissue, Rat heart tissue, Rat liver tissue. All lanes: RPS6KA5 antibody at 3µg/ml. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 90, 62, 82 kDa. Observed band size: 90 kDa.. | |
Immunohistochemistry of paraffin-embedded human placenta tissue using PACO49990 at dilution of 1:100. | |
Immunohistochemistry of paraffin-embedded human thyroid tissue using PACO49990 at dilution of 1:100. |
バックグラウンド: | Serine/threonine-protein kinase that is required for the mitogen or stress-induced phosphorylation of the transcription factors CREB1 and ATF1 and for the regulation of the transcription factors RELA, STAT3 and ETV1/ER81, and that contributes to gene activation by histone phosphorylation and functions in the regulation of inflammatory genes. Phosphorylates CREB1 and ATF1 in response to mitogenic or stress stimuli such as UV-C irradiation, epidermal growth factor (EGF) and anisomycin. Plays an essential role in the control of RELA transcriptional activity in response to TNF and upon glucocorticoid, associates in the cytoplasm with the glucocorticoid receptor NR3C1 and contributes to RELA inhibition and repression of inflammatory gene expression. In skeletal myoblasts is required for phosphorylation of RELA at 'Ser-276' during oxidative stress. In erythropoietin-stimulated cells, is necessary for the 'Ser-727' phosphorylation of STAT3 and regulation of its transcriptional potential. Phosphorylates ETV1/ER81 at 'Ser-191' and 'Ser-216', and thereby regulates its ability to stimulate transcription, which may be important during development and breast tumor formation. Directly represses transcription via phosphorylation of 'Ser-1' of histone H2A. Phosphorylates 'Ser-10' of histone H3 in response to mitogenics, stress stimuli and EGF, which results in the transcriptional activation of several immediate early genes, including proto-oncogenes c-fos/FOS and c-jun/JUN. May also phosphorylate 'Ser-28' of histone H3. Mediates the mitogen- and stress-induced phosphorylation of high mobility group protein 1 (HMGN1/HMG14). In lipopolysaccharide-stimulated primary macrophages, acts downstream of the Toll-like receptor TLR4 to limit the production of pro-inflammatory cytokines. Functions probably by inducing transcription of the MAP kinase phosphatase DUSP1 and the anti-inflammatory cytokine interleukin 10 (IL10), via CREB1 and ATF1 transcription factors. Plays a role in neuronal cell death by mediating the downstream effects of excitotoxic injury. |
シノニム: | Ribosomal protein S6 kinase alpha-5 (S6K-alpha-5) (EC 2.7.11.1) (90 kDa ribosomal protein S6 kinase 5) (Nuclear mitogen- and stress-activated protein kinase 1) (RSK-like protein kinase) (RSKL), RPS6KA5, MSK1 |
UniProt Protein Function: | MSK1: an AGC kinase of the RSK family. Has two kinase domains connected by a regulatory linker region. Enzyme activity requires the presence of both kinase domains. Is phosphorylated and activated by Erk as well as p38 MAPK in response to growth factors and cellular stress. |
UniProt Protein Details: | Protein type:EC 2.7.11.1; Protein kinase, AGC; Kinase, protein; Protein kinase, Ser/Thr (non-receptor); AGC group; RSK family; MSK subfamily Chromosomal Location of Human Ortholog: 14q31-q32.1 Cellular Component: cytoplasm; nucleoplasm; nucleus Molecular Function:ATP binding; histone serine kinase activity (H3-S10 specific); magnesium ion binding; protein binding; protein kinase activity; protein serine/threonine kinase activity Biological Process: activation of CREB transcription factor; activation of NF-kappaB transcription factor; axon guidance; epidermal growth factor receptor signaling pathway; histone phosphorylation; inflammatory response; innate immune response; MyD88-dependent toll-like receptor signaling pathway; MyD88-independent toll-like receptor signaling pathway; negative regulation of cytokine production; negative regulation of transcription, DNA-dependent; nerve growth factor receptor signaling pathway; positive regulation of histone acetylation; positive regulation of histone phosphorylation; positive regulation of transcription from RNA polymerase II promoter; protein amino acid phosphorylation; regulation of transcription, DNA-dependent; stimulatory C-type lectin receptor signaling pathway; stress-activated MAPK cascade; toll-like receptor 10 signaling pathway; toll-like receptor 2 signaling pathway; toll-like receptor 3 signaling pathway; toll-like receptor 4 signaling pathway; toll-like receptor 5 signaling pathway; toll-like receptor 9 signaling pathway; toll-like receptor signaling pathway |
UniProt Code: | O75582 |
NCBI GenInfo Identifier: | 37999482 |
NCBI Gene ID: | 9252 |
NCBI Accession: | O75582.1 |
UniProt Secondary Accession: | O75582,O95316, Q96AF7, B7Z2Y5, |
UniProt Related Accession: | O75582 |
Molecular Weight: | 81,780 Da |
NCBI Full Name: | Ribosomal protein S6 kinase alpha-5 |
NCBI Synonym Full Names: | ribosomal protein S6 kinase A5 |
NCBI Official Symbol: | RPS6KA5 |
NCBI Official Synonym Symbols: | MSK1; RLPK; MSPK1 |
NCBI Protein Information: | ribosomal protein S6 kinase alpha-5 |
UniProt Protein Name: | Ribosomal protein S6 kinase alpha-5 |
UniProt Synonym Protein Names: | 90 kDa ribosomal protein S6 kinase 5; Nuclear mitogen- and stress-activated protein kinase 1; RSK-like protein kinase; RSKL |
Protein Family: | Ribosomal protein S6 kinase |
UniProt Gene Name: | RPS6KA5 |
UniProt Entry Name: | KS6A5_HUMAN |
Secondary Antibody |
Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014) |
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