QPRT Antibody (PACO60024)
- SKU:
- PACO60024
- Product type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Application:
- ELISA
- Application:
- IHC
- Application:
- IF
- Antibody type:
- Polyclonal
- Conjugation:
- Unconjugated
Frequently bought together:
Description
抗体名: | QPRT Antibody (PACO60024) |
抗体コード: | PACO60024 |
サイズ: | 50ug |
宿主種: | Rabbit |
申し込み: | ELISA, IHC, IF |
推奨される希釈: | ELISA:1:2000-1:10000, IHC:1:200-1:500, IF:1:50-1:200 |
反応性: | Human |
免疫原: | Recombinant Human Nicotinate-nucleotide pyrophosphorylase [carboxylating] protein (113-254AA) |
憲法: | Liquid |
ストレージバッファ: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
精製方法: | >95%, Protein G purified |
抗体のクローン性: | Polyclonal |
アイソタイプ: | IgG |
Conjugate: | Non-conjugated |
IHC image of PACO60024 diluted at 1:300 and staining in paraffin-embedded human liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. | |
Immunofluorescence staining of HepG2 cells with PACO60024 at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). |
バックグラウンド: | Involved in the catabolism of quinolinic acid, (QA). |
シノニム: | Nicotinate-nucleotide pyrophosphorylase [carboxylating] (EC 2.4.2.19) (Quinolinate phosphoribosyltransferase [decarboxylating]) (QAPRTase) (QPRTase), QPRT |
UniProt Protein Function: | QPRT: Involved in the catabolism of quinolinic acid (QA). Belongs to the NadC/ModD family. |
UniProt Protein Details: | Protein type:Transferase; EC 2.4.2.19; Cofactor and Vitamin Metabolism - nicotinate and nicotinamide Chromosomal Location of Human Ortholog: 16p11.2 Cellular Component: cytoplasm; cytosol Molecular Function:nicotinate-nucleotide diphosphorylase (carboxylating) activity; protein homodimerization activity Biological Process: NAD biosynthetic process; NAD metabolic process; protein oligomerization |
NCBI Summary: | This gene encodes a key enzyme in catabolism of quinolinate, an intermediate in the tryptophan-nicotinamide adenine dinucleotide pathway. Quinolinate acts as a most potent endogenous exitotoxin to neurons. Elevation of quinolinate levels in the brain has been linked to the pathogenesis of neurodegenerative disorders such as epilepsy, Alzheimer's disease, and Huntington's disease. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2015] |
UniProt Code: | Q15274 |
NCBI GenInfo Identifier: | 296439291 |
NCBI Gene ID: | 23475 |
NCBI Accession: | Q15274.3 |
UniProt Secondary Accession: | Q15274,Q53XW7, Q96G22, Q9BSG6, |
UniProt Related Accession: | Q15274 |
Molecular Weight: | 30,846 Da |
NCBI Full Name: | Nicotinate-nucleotide pyrophosphorylase |
NCBI Synonym Full Names: | quinolinate phosphoribosyltransferase |
NCBI Official Symbol: | QPRT |
NCBI Official Synonym Symbols: | QPRTase; HEL-S-90n |
NCBI Protein Information: | nicotinate-nucleotide pyrophosphorylase [carboxylating] |
UniProt Protein Name: | Nicotinate-nucleotide pyrophosphorylase [carboxylating] |
UniProt Synonym Protein Names: | Quinolinate phosphoribosyltransferase [decarboxylating]; QAPRTase; QPRTase |
Protein Family: | Nicotinate-nucleotide pyrophosphorylase |
UniProt Gene Name: | QPRT |
UniProt Entry Name: | NADC_HUMAN |
Antibodies | ELISA Kits |
Anti-QPRT Antibody (CAB14349) | Human QPRT (Quinolinate phosphoribosyltransferase) ELISA Kit (HUFI08578) |
Secondary Antibody |
Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014) |
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Anti-FITC Goat Anti-Rabbit IgG (H+L) Antibody (CABS011) |
Anti-HRP-conjugated Beta Actin Antibody (CABC028) |