NT5E Antibody (PACO62175)
- SKU:
- PACO62175
- Product type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Application:
- ELISA
- Application:
- WB
- Application:
- IHC
- Antibody type:
- Polyclonal
- Conjugation:
- Unconjugated
Description
抗体名: | NT5E Antibody (PACO62175) |
抗体コード: | PACO62175 |
サイズ: | 50ul |
宿主種: | Rabbit |
申し込み: | ELISA, WB, IHC |
推奨される希釈: | ELISA:1:2000-1:10000, WB:1:1000-1:5000, IHC:1:20-1:200 |
反応性: | Human |
免疫原: | Recombinant Human 5'-nucleotidase protein (32-264AA) |
憲法: | Liquid |
ストレージバッファ: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
精製方法: | Antigen Affinity Purified |
抗体のクローン性: | Polyclonal |
アイソタイプ: | IgG |
Conjugate: | Non-conjugated |
Western Blot. Positive WB detected in: K562 whole cell lysate, Jurkat whole cell lysate, U87 whole cell lysate, U251 whole cell lysate, PC3 whole cell lysate, HUVEC whole cell lysate. All lanes: NT5E antibody at 1:2000. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 64, 58 kDa. Observed band size: 64 kDa. | |
IHC image of PACO62175 diluted at 1:30 and staining in paraffin-embedded human skeletal muscle tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
バックグラウンド: | Hydrolyzes extracellular nucleotides into membrane permeable nucleosides. Exhibits AMP-, NAD-, and NMN-nucleosidase activities. |
シノニム: | 5'-nucleotidase (5'-NT) (EC 3.1.3.5) (Ecto-5'-nucleotidase) (CD antigen CD73), NT5E, NT5 NTE |
UniProt Protein Function: | NT5E: Hydrolyzes extracellular nucleotides into membrane permeable nucleosides. Homodimer; disulfide-linked. Belongs to the 5'-nucleotidase family. 2 isoforms of the human protein are produced by alternative splicing. |
UniProt Protein Details: | Protein type:EC 3.1.3.5; Membrane protein, integral; Nucleotide Metabolism - pyrimidine; Nucleotide Metabolism - purine; Phosphatase (non-protein); Membrane protein, GPI anchor; Cofactor and Vitamin Metabolism - nicotinate and nicotinamide Chromosomal Location of Human Ortholog: 6q14-q21 Cellular Component: cell surface; membrane; cytoplasm; plasma membrane Molecular Function:5'-nucleotidase activity; metal ion binding; nucleotide binding Biological Process: pyrimidine nucleoside catabolic process; dephosphorylation; pyrimidine base metabolic process; nucleobase, nucleoside and nucleotide metabolic process; adenosine biosynthetic process; negative regulation of inflammatory response; purine nucleotide catabolic process; DNA metabolic process; purine base metabolic process; AMP catabolic process Disease: Calcification Of Joints And Arteries |
NCBI Summary: | The protein encoded by this gene is a plasma membrane protein that catalyzes the conversion of extracellular nucleotides to membrane-permeable nucleosides. The encoded protein is used as a determinant of lymphocyte differentiation. Defects in this gene can lead to the calcification of joints and arteries. Two transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Mar 2011] |
UniProt Code: | P21589 |
NCBI GenInfo Identifier: | 112825 |
NCBI Gene ID: | 4907 |
NCBI Accession: | P21589.1 |
UniProt Related Accession: | P21589 |
Molecular Weight: | |
NCBI Full Name: | 5'-nucleotidase |
NCBI Synonym Full Names: | 5'-nucleotidase ecto |
NCBI Official Symbol: | NT5E |
NCBI Official Synonym Symbols: | NT; eN; NT5; NTE; eNT; CD73; E5NT; CALJA |
NCBI Protein Information: | 5'-nucleotidase |
UniProt Protein Name: | 5'-nucleotidase |
UniProt Synonym Protein Names: | Ecto-5'-nucleotidase; CD_antigen: CD73 |
UniProt Gene Name: | NT5E |
UniProt Entry Name: | 5NTD_HUMAN |
Secondary Antibody |
Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014) |
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