METTL14 Antibody (PACO48154)
- SKU:
- PACO48154
- Product type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Application:
- ELISA
- Application:
- WB
- Application:
- IHC
- Antibody type:
- Polyclonal
- Conjugation:
- Unconjugated
Frequently bought together:
Description
抗体名: | METTL14 Antibody (PACO48154) |
抗体コード: | PACO48154 |
サイズ: | 50ug |
宿主種: | Rabbit |
申し込み: | ELISA, WB, IHC |
推奨される希釈: | ELISA:1:2000-1:10000, WB:1:2000-1:10000, IHC:1:20-1:200 |
反応性: | Human |
免疫原: | Recombinant Human N6-adenosine-methyltransferase subunit METTL14 protein (24-206AA) |
憲法: | Liquid |
ストレージバッファ: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
精製方法: | >95%, Protein G purified |
抗体のクローン性: | Polyclonal |
アイソタイプ: | IgG |
Conjugate: | Non-conjugated |
Western Blot. Positive WB detected in: SH-SY5Y whole cell lysate. All lanes: METTL14 antibody at 3µg/ml. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 53 kDa. Observed band size: 53 kDa.. | |
Immunohistochemistry of paraffin-embedded human testis tissue using PACO48154 at dilution of 1:100. | |
Immunohistochemistry of paraffin-embedded human gastric cancer using PACO48154 at dilution of 1:100. |
バックグラウンド: | N6-methyltransferase that methylates adenosine residues of some mRNAs and acts as a regulator of the circadian clock and differentiation of embryonic stem cells. N6-methyladenosine (m6A), which takes place at the 5'-[AG]GAC-3' consensus sites of some mRNAs, plays a role in the efficiency of mRNA splicing, processing and mRNA stability. M6A regulates the length of the circadian clock: acts as a early pace-setter in the circadian loop. M6A also acts as a regulator of mRNA stability: in embryonic stem cells (ESCs), m6A methylation of mRNAs encoding key naive pluripotency-promoting transcripts results in transcript destabilization (By similarity). |
シノニム: | N6-adenosine-methyltransferase non-catalytic subunit (Methyltransferase-like protein 14) (hMETTL14), METTL14, KIAA1627 |
UniProt Protein Function: | The METTL3-METTL14 heterodimer forms a N6-methyltransferase complex that methylates adenosine residues at the N6 position of some mRNAs and regulates the circadian clock, differentiation of embryonic stem cells and cortical neurogenesis (PubMed:24316715, PubMed:24407421, PubMed:25719671, PubMed:27373337, PubMed:27281194). In the heterodimer formed with METTL3, METTL14 constitutes the RNA-binding scaffold that recognizes the substrate rather than the catalytic core (PubMed:27627798, PubMed:27373337, PubMed:27281194). N6-methyladenosine (m6A), which takes place at the 5'-[AG]GAC-3' consensus sites of some mRNAs, plays a role in mRNA stability and processing (PubMed:24316715, PubMed:24407421, PubMed:25719671). M6A acts as a key regulator of mRNA stability by promoting mRNA destabilization and degradation. In embryonic stem cells (ESCs), m6A methylation of mRNAs encoding key naive pluripotency-promoting transcripts results in transcript destabilization. M6A regulates spermatogonial differentiation and meiosis and is essential for male fertility and spermatogenesis. M6A also regulates cortical neurogenesis: m6A methylation of transcripts related to transcription factors, neural stem cells, the cell cycle and neuronal differentiation during brain development promotes their destabilization and decay, promoting differentiation of radial glial cells. |
UniProt Code: | Q9HCE5 |
NCBI GenInfo Identifier: | 24308265 |
NCBI Gene ID: | 57721 |
NCBI Accession: | NP_066012.1 |
UniProt Secondary Accession: | Q9HCE5,Q969V2, A6NIG1, |
UniProt Related Accession: | Q9HCE5 |
Molecular Weight: | 52150 |
NCBI Full Name: | N6-adenosine-methyltransferase non-catalytic subunit |
NCBI Synonym Full Names: | methyltransferase like 14 |
NCBI Official Symbol: | METTL14 |
NCBI Official Synonym Symbols: | hMETTL14 |
NCBI Protein Information: | N6-adenosine-methyltransferase non-catalytic subunit; N6-adenosine-methyltransferase subunit METTL14 |
UniProt Protein Name: | N6-adenosine-methyltransferase non-catalytic subunit |
UniProt Synonym Protein Names: | Methyltransferase-like protein 14 |
Protein Family: | N6-adenosine-methyltransferase |
UniProt Gene Name: | METTL14 |
Antibodies |
Anti-METTL14 Antibody (CAB8530) |
Secondary Antibody |
Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014) |
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Anti-HRP-conjugated Beta Actin Antibody (CABC028) |