IST1 Antibody (PACO57828)
- SKU:
- PACO57828
- Product type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Application:
- ELISA
- Application:
- IHC
- Application:
- IF
- Antibody type:
- Polyclonal
- Conjugation:
- Unconjugated
Frequently bought together:
Description
抗体名: | IST1 Antibody (PACO57828) |
抗体コード: | PACO57828 |
サイズ: | 50ug |
宿主種: | Rabbit |
申し込み: | ELISA, IHC, IF |
推奨される希釈: | ELISA:1:2000-1:10000, IHC:1:200-1:500, IF:1:50-1:200 |
反応性: | Human |
免疫原: | Recombinant Human IST1 homolog protein (172-284AA) |
憲法: | Liquid |
ストレージバッファ: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
精製方法: | >95%, Protein G purified |
抗体のクローン性: | Polyclonal |
アイソタイプ: | IgG |
Conjugate: | Non-conjugated |
IHC image of PACO57828 diluted at 1:200 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. | |
Immunofluorescence staining of HepG2 cells with PACO57828 at 1:66, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). |
バックグラウンド: | ESCRT-III-like protein involved in specific functions of the ESCRT machinery. Is required for efficient abscission during cytokinesis, but not for HIV-1 budding. The involvement in the MVB pathway is not established. Involved in recruiting VPS4A and/or VPS4B to the midbody of dividing cells. During late anaphase, involved in nuclear envelope reassembly and mitotic spindle disassembly together with the ESCRT-III complex: IST1 acts by mediating the recruitment of SPAST to the nuclear membrane, leading to microtubule severing. Regulates early endosomal tubulation together with the ESCRT-III complex by mediating the recruitment of SPAST. |
シノニム: | IST1 homolog (hIST1) (Putative MAPK-activating protein PM28), IST1, KIAA0174 |
UniProt Protein Function: | IST1: Proposed to be involved in specific functions of the ESCRT machinery. Is required for efficient abscission during cytokinesis, but not for HIV-1 budding. The involvement in the MVB pathway is not established. Involved in recruiting VPS4A and/or VPS4B to the midbody of dividing cells. Belongs to the IST1 family. 4 isoforms of the human protein are produced by alternative splicing. |
UniProt Protein Details: | Protein type:Vesicle; Cell cycle regulation Chromosomal Location of Human Ortholog: 16q22.2 Cellular Component: centrosome; cytoplasmic membrane-bound vesicle; ER-Golgi intermediate compartment; midbody; cytosol Molecular Function:protein domain specific binding; protein binding; protein complex binding Biological Process: release of virus from host; protein localization; establishment of protein localization; cell division; positive regulation of proteolysis; abscission; cytokinesis; viral capsid re-envelopment; positive regulation of collateral sprouting |
NCBI Summary: | This gene encodes a protein with MIT-interacting motifs that interacts with components of endosomal sorting complexes required for transport (ESCRT). ESCRT functions in vesicle budding, such as that which occurs during membrane abscission in cytokinesis. There is a pseudogene for this gene on chromosome 19. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Aug 2012] |
UniProt Code: | P53990 |
NCBI GenInfo Identifier: | 41281489 |
NCBI Gene ID: | 9798 |
NCBI Accession: | NP_055576.2 |
UniProt Related Accession: | P53990 |
Molecular Weight: | 23kDa; 36kDa; 39kDa; 41kDa |
NCBI Full Name: | IST1 homolog isoform b |
NCBI Synonym Full Names: | IST1 factor associated with ESCRT-III |
NCBI Official Symbol: | IST1 |
NCBI Official Synonym Symbols: | OLC1; CHMP8 |
NCBI Protein Information: | IST1 homolog |
UniProt Protein Name: | IST1 homolog |
UniProt Synonym Protein Names: | Putative MAPK-activating protein PM28 |
Protein Family: | IST1 |
UniProt Gene Name: | IST1 |
UniProt Entry Name: | IST1_HUMAN |
Antibodies |
Anti-IST1 Antibody (CAB9401) |
Secondary Antibody |
Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014) |
Recommended Products |
Anti-FITC Goat Anti-Rabbit IgG (H+L) Antibody (CABS011) |
Anti-HRP-conjugated Beta Actin Antibody (CABC028) |