GDI1 Antibody (PACO55582)
- SKU:
- PACO55582
- Product type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Application:
- ELISA
- Application:
- WB
- Application:
- IHC
- Application:
- IF
- Antibody type:
- Polyclonal
- Conjugation:
- Unconjugated
Frequently bought together:
Description
抗体名: | GDI1 Antibody (PACO55582) |
抗体コード: | PACO55582 |
サイズ: | 50ug |
宿主種: | Rabbit |
申し込み: | ELISA, WB, IHC, IF |
推奨される希釈: | ELISA:1:2000-1:10000, WB:1:500-1:5000, IHC:1:200-1:500, IF:1:50-1:200 |
反応性: | Human |
免疫原: | Recombinant Human Rab GDP dissociation inhibitor α protein (35-170AA) |
憲法: | Liquid |
ストレージバッファ: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
精製方法: | >95%, Protein G purified |
抗体のクローン性: | Polyclonal |
アイソタイプ: | IgG |
Conjugate: | Non-conjugated |
Western Blot. Positive WB detected in: A549 whole cell lysate. All lanes: GDI1 antibody at 6µg/ml. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 51 kDa. Observed band size: 51 kDa. | |
IHC image of PACO55582 diluted at 1:200 and staining in paraffin-embedded human prostate cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. | |
IHC image of PACO55582 diluted at 1:200 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
バックグラウンド: | Regulates the GDP/GTP exchange reaction of most Rab proteins by inhibiting the dissociation of GDP from them, and the subsequent binding of GTP to them. Promotes the dissociation of GDP-bound Rab proteins from the membrane and inhibits their activation. Promotes the dissociation of RAB1A, RAB3A, RAB5A and RAB10 from membranes. |
シノニム: | Rab GDP dissociation inhibitor α (Rab GDI alpha) (Guanosine diphosphate dissociation inhibitor 1) (GDI-1) (Oligophrenin-2) (Protein XAP-4), GDI1, GDIL OPHN2 RABGDIA XAP4 |
UniProt Protein Function: | GDI1: a GDP dissociation inhibitor protein expressed primarily in neural and sensory tissues. GDP dissociation inhibitors are proteins that regulate the GDP-GTP exchange reaction of members of the Rab family, small GTP-binding proteins of the Ras superfamily, that are involved in vesicular trafficking of molecules between cellular organelles. GDIs slow the rate of dissociation of GDP from Rab proteins and release GDP from membrane-bound Rabs. Mutations have been linked to X-linked nonspecific mental retardation. |
UniProt Protein Details: | Protein type:Motility/polarity/chemotaxis; G protein regulator, misc. Chromosomal Location of Human Ortholog: Xq28 Cellular Component: Golgi apparatus; neuron projection; protein complex; cytoplasm; midbody; cytosol Molecular Function:protein binding; GDP-dissociation inhibitor activity; Rab GDP-dissociation inhibitor activity; GTPase activator activity; Rab GTPase binding Biological Process: protein transport; regulation of small GTPase mediated signal transduction; small GTPase mediated signal transduction; negative regulation of axonogenesis; signal transduction; Rab protein signal transduction; response to calcium ion; positive regulation of GTPase activity Disease: Mental Retardation, X-linked 41 |
NCBI Summary: | GDP dissociation inhibitors are proteins that regulate the GDP-GTP exchange reaction of members of the rab family, small GTP-binding proteins of the ras superfamily, that are involved in vesicular trafficking of molecules between cellular organelles. GDIs slow the rate of dissociation of GDP from rab proteins and release GDP from membrane-bound rabs. GDI1 is expressed primarily in neural and sensory tissues. Mutations in GDI1 have been linked to X-linked nonspecific mental retardation. [provided by RefSeq, Jul 2008] |
UniProt Code: | P31150 |
NCBI GenInfo Identifier: | 1707886 |
NCBI Gene ID: | 2664 |
NCBI Accession: | P31150.2 |
UniProt Secondary Accession: | P31150,P50394, Q6FG50, Q7Z2G6, Q7Z2G9, Q7Z2H5, Q7Z2I6 |
UniProt Related Accession: | P31150 |
Molecular Weight: | 447 |
NCBI Full Name: | Rab GDP dissociation inhibitor alpha |
NCBI Synonym Full Names: | GDP dissociation inhibitor 1 |
NCBI Official Symbol: | GDI1 |
NCBI Official Synonym Symbols: | 1A; GDIL; MRX41; MRX48; OPHN2; XAP-4; RABGD1A; RABGDIA |
NCBI Protein Information: | rab GDP dissociation inhibitor alpha; GDI-1; protein XAP-4; rab GDI alpha; oligophrenin-2; mental retardation, X-linked 48; rab GDP-dissociation inhibitor, alpha; guanosine diphosphate dissociation inhibitor 1 |
UniProt Protein Name: | Rab GDP dissociation inhibitor alpha |
UniProt Synonym Protein Names: | Guanosine diphosphate dissociation inhibitor 1; GDI-1; Oligophrenin-2; Protein XAP-4 |
Protein Family: | Guanosine nucleotide diphosphate dissociation inhibitor |
UniProt Gene Name: | GDI1 |
UniProt Entry Name: | GDIA_HUMAN |
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