EPHA5 Antibody (PACO49574)
- SKU:
- PACO49574
- Product type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Application:
- ELISA
- Application:
- WB
- Application:
- IHC
- Application:
- IF
- Antibody type:
- Polyclonal
- Conjugation:
- Unconjugated
Frequently bought together:
Description
抗体名: | EPHA5 Antibody (PACO49574) |
抗体コード: | PACO49574 |
サイズ: | 50ug |
宿主種: | Rabbit |
申し込み: | ELISA, WB, IHC, IF |
推奨される希釈: | ELISA:1:2000-1:10000, WB:1:500-1:5000, IHC:1:20-1:200, IF:1:50-1:200 |
反応性: | Human |
免疫原: | Recombinant Human Ephrin type-A receptor 5 protein (246-514AA) |
憲法: | Liquid |
ストレージバッファ: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
精製方法: | >95%, Protein G purified |
抗体のクローン性: | Polyclonal |
アイソタイプ: | IgG |
Conjugate: | Non-conjugated |
Western Blot. Positive WB detected in: A549 whole cell lysate, Hela whole cell lysate. All lanes: EPHA5 antibody at 3µg/ml. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 115, 113, 108 kDa. Observed band size: 115 kDa.. | |
Immunofluorescent analysis of A549 cells using PACO49574 at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). | |
Immunohistochemistry of paraffin-embedded human brain tissue using PACO49574 at dilution of 1:100. |
バックグラウンド: | Receptor tyrosine kinase which binds promiscuously GPI-anchored ephrin-A family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Among GPI-anchored ephrin-A ligands, EFNA5 most probably constitutes the cognate/functional ligand for EPHA5. Functions as an axon guidance molecule during development and may be involved in the development of the retinotectal, entorhino-hippocampal and hippocamposeptal pathways. Together with EFNA5 plays also a role in synaptic plasticity in adult brain through regulation of synaptogenesis. In addition to its function in the nervous system, the interaction of EPHA5 with EFNA5 mediates communication between pancreatic islet cells to regulate glucose-stimulated insulin secretion (By similarity). |
シノニム: | Ephrin type-A receptor 5 (EC 2.7.10.1) (Brain-specific kinase) (EPH homology kinase 1) (EHK-1) (EPH-like kinase 7) (EK7) (hEK7), EPHA5, BSK EHK1 HEK7 TYRO4 |
UniProt Protein Function: | EphA5: Receptor tyrosine kinase which binds promiscuously GPI- anchored ephrin-A family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Among GPI-anchored ephrin-A ligands, EFNA5 most probably constitutes the cognate/functional ligand for EPHA5. Functions as an axon guidance molecule during development and may be involved in the development of the retinotectal, entorhino- hippocampal and hippocamposeptal pathways. Together with EFNA5 plays also a role in synaptic plasticity in adult brain through regulation of synaptogenesis. Beside its function in the nervous system, the interaction of EPHA5 with EFNA5 mediates communication between pancreatic islet cells to regulate glucose-stimulated insulin secretion. Belongs to the protein kinase superfamily. Tyr protein kinase family. Ephrin receptor subfamily. 3 isoforms of the human protein are produced by alternative splicing. |
UniProt Protein Details: | Protein type:Protein kinase, tyrosine (receptor); Kinase, protein; Membrane protein, integral; Protein kinase, TK; EC 2.7.10.1; TK group; Eph family Chromosomal Location of Human Ortholog: 4q13.1 Cellular Component: cell soma; dendrite; external side of plasma membrane; perinuclear region of cytoplasm; plasma membrane; rough endoplasmic reticulum Molecular Function:ephrin receptor activity; GPI-linked ephrin receptor activity Biological Process: activation of CREB transcription factor; axon guidance; cAMP-mediated signaling; ephrin receptor signaling pathway; hippocampus development; neuron development; regulation of actin cytoskeleton organization and biogenesis; regulation of GTPase activity |
NCBI Summary: | This gene belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family. EPH and EPH-related receptors have been implicated in mediating developmental events, particularly in the nervous system. Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. The ephrin receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Aug 2013] |
UniProt Code: | P54756 |
NCBI GenInfo Identifier: | 259016353 |
NCBI Gene ID: | 2044 |
NCBI Accession: | P54756.3 |
UniProt Secondary Accession: | P54756,Q7Z3F2, |
UniProt Related Accession: | P54756 |
Molecular Weight: | 107,699 Da |
NCBI Full Name: | Ephrin type-A receptor 5 |
NCBI Synonym Full Names: | EPH receptor A5 |
NCBI Official Symbol: | EPHA5 |
NCBI Official Synonym Symbols: | EK7; CEK7; EHK1; HEK7; EHK-1; TYRO4 |
NCBI Protein Information: | ephrin type-A receptor 5 |
UniProt Protein Name: | Ephrin type-A receptor 5 |
UniProt Synonym Protein Names: | Brain-specific kinase; EPH homology kinase 1; EHK-1; EPH-like kinase 7; EK7; hEK7 |
Protein Family: | Ephrin type-A receptor |
UniProt Gene Name: | EPHA5 |
UniProt Entry Name: | EPHA5_HUMAN |
Antibodies | ELISA Kits |
Anti-EPHA5 Antibody (CAB14238) | EPHA5 Colorimetric Cell-Based ELISA Kit |
EPHA5 Antibody (PACO06465) |
Secondary Antibody |
Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014) |
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