CHFR Antibody, Biotin conjugated (PACO48029)
- SKU:
- PACO48029
- Product type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Application:
- ELISA
- Antibody type:
- Polyclonal
- Conjugation:
- Biotin
Frequently bought together:
Description
抗体名: | CHFR Antibody, Biotin conjugated (PACO48029) |
抗体コード: | PACO48029 |
サイズ: | 50ug |
宿主種: | Rabbit |
申し込み: | ELISA |
推奨される希釈: | |
反応性: | Human |
免疫原: | Recombinant Human E3 ubiquitin-protein ligase CHFR protein (141-422AA) |
憲法: | Liquid |
ストレージバッファ: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
精製方法: | >95%, Protein G purified |
抗体のクローン性: | Polyclonal |
アイソタイプ: | IgG |
Conjugate: | Biotin |
バックグラウンド: | E3 ubiquitin-protein ligase that functions in the antephase checkpoint by actively delaying passage into mitosis in response to microtubule poisons. Acts in early prophase before chromosome condensation, when the centrosome move apart from each other along the periphery of the nucleus. Probably involved in signaling the presence of mitotic stress caused by microtubule poisons by mediating the 'Lys-48'-linked ubiquitination of target proteins, leading to their degradation by the proteasome. Promotes the ubiquitination and subsequent degradation of AURKA and PLK1. Probably acts as a tumor suppressor, possibly by mediating the polyubiquitination of HDAC1, leading to its degradation. May also promote the formation of 'Lys-63'-linked polyubiquitin chains and functions with the specific ubiquitin-conjugating UBC13-MMS2 (UBE2N-UBE2V2) heterodimer. Substrates that are polyubiquitinated at 'Lys-63' are usually not targeted for degradation, but are rather involved in signaling cellular stress. |
シノニム: | E3 ubiquitin-protein ligase CHFR (EC 2.3.2.27) (Checkpoint with forkhead and RING finger domains protein) (RING finger protein 196) (RING-type E3 ubiquitin transferase CHFR), CHFR, RNF196 |
UniProt Protein Function: | CHFR: E3 ubiquitin-protein ligase that functions in the antephase checkpoint by actively delaying passage into mitosis in response to microtubule poisons. Acts in early prophase before chromosome condensation, when the centrosome move apart from each other along the periphery of the nucleus. Probably involved in signaling the presence of mitotic stress caused by microtubule poisons by mediating the 'Lys-48'-linked ubiquitination of target proteins, leading to their degradation by the proteasome. Promotes the ubiquitination and subsequent degradation of AURKA and PLK1. Probably acts as a tumor suppressor, possibly by mediating the polyubiquitination of HDAC1, leading to its degradation. May also promote the formation of 'Lys-63'-linked polyubiquitin chains and functions with the specific ubiquitin-conjugating UBC13-MMS2 (UBE2N-UBE2V2) heterodimer. Substrates that are polyubiquitinated at 'Lys-63' are usually not targeted for degradation, but are rather involved in signaling cellular stress. Interacts with HDAC1 and HDAC2. Interacts with PML (with sumoylated form of PML). Ubiquitous. Belongs to the CHFR family. 5 isoforms of the human protein are produced by alternative splicing. |
UniProt Protein Details: | Protein type:Ligase; EC 6.3.2.19; Ubiquitin conjugating system; EC 6.3.2.-; Ubiquitin ligase Chromosomal Location of Human Ortholog: 12q24.33 Cellular Component: PML body; nucleus Molecular Function:protein binding; zinc ion binding; ubiquitin-protein ligase activity; nucleotide binding; ligase activity Biological Process: ubiquitin-dependent protein catabolic process; modification-dependent protein catabolic process; mitosis; protein polyubiquitination; cell division; mitotic cell cycle checkpoint |
NCBI Summary: | This gene encodes an E3 ubiquitin-protein ligase required for the maintenance of the antephase checkpoint that regulates cell cycle entry into mitosis and, therefore, may play a key role in cell cycle progression and tumorigenesis. The encoded protein has an N-terminal forkhead-associated domain, a central RING-finger domain, and a cysteine-rich C-terminal region. Alternatively spliced transcript variants that encode different protein isoforms have been described. [provided by RefSeq, Mar 2014] |
UniProt Code: | Q96EP1 |
NCBI GenInfo Identifier: | 41688511 |
NCBI Gene ID: | 55743 |
NCBI Accession: | Q96EP1.2 |
UniProt Secondary Accession: | Q96EP1,Q96SL3, Q9NRT4, Q9NT32, Q9NVD5, A6NEN5, B4DZ77 B4E2L6, |
UniProt Related Accession: | Q96EP1 |
Molecular Weight: | 664 |
NCBI Full Name: | E3 ubiquitin-protein ligase CHFR |
NCBI Synonym Full Names: | checkpoint with forkhead and ring finger domains, E3 ubiquitin protein ligase |
NCBI Official Symbol: | CHFR |
NCBI Official Synonym Symbols: | RNF116; RNF196 |
NCBI Protein Information: | E3 ubiquitin-protein ligase CHFR; RING finger protein 196 |
UniProt Protein Name: | E3 ubiquitin-protein ligase CHFR |
UniProt Synonym Protein Names: | Checkpoint with forkhead and RING finger domains protein; RING finger protein 196 |
Protein Family: | E3 ubiquitin-protein ligase |
UniProt Gene Name: | CHFR |
UniProt Entry Name: | CHFR_HUMAN |