C1GALT1 Antibody (PACO58560)
- SKU:
- PACO58560
- Product type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Application:
- ELISA
- Application:
- WB
- Application:
- IHC
- Application:
- IF
- Antibody type:
- Polyclonal
- Conjugation:
- Unconjugated
Frequently bought together:
Description
抗体名: | C1GALT1 Antibody (PACO58560) |
抗体コード: | PACO58560 |
サイズ: | 50ug |
宿主種: | Rabbit |
申し込み: | ELISA, WB, IHC, IF |
推奨される希釈: | ELISA:1:2000-1:10000, WB:1:500-1:5000, IHC:1:500-1:1000, IF:1:200-1:500 |
反応性: | Human |
免疫原: | Recombinant Human Glycoprotein-N-acetylgalactosamine 3-beta-galactosyltransferase 1 protein (34-143AA) |
憲法: | Liquid |
ストレージバッファ: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
精製方法: | >95%, Protein G purified |
抗体のクローン性: | Polyclonal |
アイソタイプ: | IgG |
Conjugate: | Non-conjugated |
Western Blot. Positive WB detected in: U251 whole cell lysate, HepG2 whole cell lysate. All lanes: C1GALT1 antibody at 4.4µg/ml. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 43, 36 kDa. Observed band size: 43 kDa. | |
IHC image of PACO58560 diluted at 1:1000 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. | |
IHC image of PACO58560 diluted at 1:1000 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
バックグラウンド: | Glycosyltransferase that generates the core 1 O-glycan Gal-beta1-3GalNAc-alpha1-Ser/Thr (T antigen), which is a precursor for many extended O-glycans in glycoproteins. Plays a central role in many processes, such as angiogenesis, thrombopoiesis and kidney homeostasis development. |
シノニム: | Glycoprotein-N-acetylgalactosamine 3-beta-galactosyltransferase 1 (EC 2.4.1.122) (B3Gal-T8) (Core 1 O-glycan T-synthase) (Core 1 UDP-galactose:N-acetylgalactosamine-alpha-R β 1,3-galactosyltransferase 1) (Beta-1,3-galactosyltransferase) (Core 1 beta1,3-galactosyltransferase 1) (C1GalT1) (Core 1 beta3-Gal-T1), C1GALT1 |
UniProt Protein Function: | C1GALT1: Glycosyltransferase that generates the core 1 O-glycan Gal-beta1-3GalNAc-alpha1-Ser/Thr (T antigen), which is a precursor for many extended O-glycans in glycoproteins. Plays a central role in many processes, such as angiogenesis, thrombopoiesis and kidney homeostasis development. Belongs to the glycosyltransferase 31 family. Beta3- Gal-T subfamily. 2 isoforms of the human protein are produced by alternative splicing. |
UniProt Protein Details: | Protein type:EC 2.4.1.122; Glycan Metabolism - O-glycan biosynthesis; Transferase; Membrane protein, integral Chromosomal Location of Human Ortholog: 7p21.3 Cellular Component: Golgi membrane; integral to membrane Molecular Function:glycoprotein-N-acetylgalactosamine 3-beta-galactosyltransferase activity; protein binding Biological Process: angiogenesis; kidney development; O-glycan processing; O-glycan processing, core 1 |
NCBI Summary: | The protein encoded by this gene generates the common core 1 O-glycan structure, Gal-beta-1-3GalNAc-R, by the transfer of Gal from UDP-Gal to GalNAc-alpha-1-R. Core 1 is a precursor for many extended mucin-type O-glycans on cell surface and secreted glycoproteins. Studies in mice suggest that this gene plays a key role in thrombopoiesis and kidney homeostasis.[provided by RefSeq, Sep 2010] |
UniProt Code: | Q9NS00 |
NCBI GenInfo Identifier: | 74719147 |
NCBI Gene ID: | 56913 |
NCBI Accession: | Q9NS00.1 |
UniProt Secondary Accession: | Q9NS00,Q96QH4, Q9BTU1, |
UniProt Related Accession: | Q9NS00 |
Molecular Weight: | 35,935 Da |
NCBI Full Name: | Glycoprotein-N-acetylgalactosamine 3-beta-galactosyltransferase 1 |
NCBI Synonym Full Names: | core 1 synthase, glycoprotein-N-acetylgalactosamine 3-beta-galactosyltransferase 1 |
NCBI Official Symbol: | C1GALT1 |
NCBI Official Synonym Symbols: | C1GALT; T-synthase |
NCBI Protein Information: | glycoprotein-N-acetylgalactosamine 3-beta-galactosyltransferase 1 |
UniProt Protein Name: | Glycoprotein-N-acetylgalactosamine 3-beta-galactosyltransferase 1 |
UniProt Synonym Protein Names: | B3Gal-T8; Core 1 O-glycan T-synthase; Core 1 UDP-galactose:N-acetylgalactosamine-alpha-R beta 1,3-galactosyltransferase 1; Beta-1,3-galactosyltransferase; Core 1 beta1,3-galactosyltransferase 1; C1GalT1; Core 1 beta3-Gal-T1 |
Protein Family: | C1GALT1-specific chaperone |
UniProt Gene Name: | C1GALT1 |
UniProt Entry Name: | C1GLT_HUMAN |
Antibodies | ELISA Kits |
Anti-C1GALT1 Antibody (CAB12865) | Human Glycoprotein-N-acetylgalactosamine 3-beta-galactosyltransferase 1 (C1GALT1) ELISA Kit |
Secondary Antibody |
Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014) |
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Anti-HRP-conjugated Beta Actin Antibody (CABC028) |