Anti-SUZ12 Antibody (RACO0292)
- SKU:
- RACO0292
- Product type:
- Recombinant Antibody
- Reactivity:
- Human
- Host Species:
- Human
- Isotype:
- IgG
- Application:
- IP
- Application:
- WB
- Application:
- ELISA
- Application:
- IHC
- Conjugation:
- Unconjugated
Description
商品名: | SUZ12 Antibody |
Product SKU: | RACO0292 |
サイズ: | 50ul |
宿主種: | Homo sapiens (Human) |
申し込み: | ELISA, WB, IHC, IP |
推奨される希釈: | WB:1:500-1:5000, IHC:1:50-1:200, IP:1:200-1:1000 |
反応性: | Human |
免疫原: | A synthesized peptide derived from human SUZ12 |
憲法: | Liquid |
ストレージバッファ: | Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
精製方法: | Affinity-chromatography |
抗体のクローン性: | Monoclonal |
アイソタイプ: | Rabbit IgG |
Conjugate: | Non-conjugated |
バックグラウンド: | Polycomb group (PcG) protein. Component of the PRC2/EED-EZH2 complex, which methylates 'Lys-9' (H3K9me) and 'Lys-27' (H3K27me) of histone H3, leading to transcriptional repression of the affected target gene. The PRC2/EED-EZH2 complex may also serve as a recruiting platform for DNA methyltransferases, thereby linking two epigenetic repression systems. Genes repressed by the PRC2/EED-EZH2 complex include HOXC8, HOXA9, MYT1 and CDKN2A. |
シノニム: | Polycomb protein SUZ12 (Chromatin precipitated E2F target 9 protein) (ChET 9 protein) (Joined to JAZF1 protein) (Suppressor of zeste 12 protein homolog), SUZ12, CHET9 JJAZ1 KIAA0160 |
IHC image of RACO0292 diluted at 1:100 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB. | |
Immunoprecipitating SUZ12 in K562 whole cell lysate Lane 1: Rabbit control IgG instead of RACO0292 in K562 whole cell lysate For western blotting,a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000). Lane 2: RACO0292(2µg)+ K562 whole cell lysate (500µg). Lane 3: K562 whole cell lysate (10µg). | |
Western Blot. Positive WB detected in Hela whole cell lysate HepG2 whole cell lysate MCF-7 whole cell lysate HL60 whole cell lysate THP-1 whole cell lysate K562 whole cell lysate All lanes SUZ12 antibody at 1:2000. Secondary. Goat polyclonal to rabbit IgG at 1:50000 dilution. Predicted band size: 84 kDa. Observed band size: 90 kDa. |
UniProt Protein Function: | SUZ12: a zinc finger protein. A breakpoint translocation of the cognate gene with JAZF1 has been reported in endometrial stromal sarcoma. |
UniProt Protein Details: | Protein type:Transcription factor; Oncoprotein; C2H2-type zinc finger protein Chromosomal Location of Human Ortholog: 17q11.2 Cellular Component: nucleoplasm; ESC/E(Z) complex; sex chromatin; nucleolus; nucleus Molecular Function:histone methyltransferase activity; protein binding; sequence-specific DNA binding; RNA binding; metal ion binding; chromatin binding; methylated histone residue binding Biological Process: histone methylation; negative regulation of cell differentiation; transcription, DNA-dependent; negative regulation of gene expression, epigenetic; positive regulation of cell proliferation; histone ubiquitination; gene expression; negative regulation of transcription from RNA polymerase II promoter; regulation of gene expression, epigenetic |
NCBI Summary: | This zinc finger gene has been identified at the breakpoints of a recurrent chromosomal translocation reported in endometrial stromal sarcoma. Recombination of these breakpoints results in the fusion of this gene and JAZF1. The protein encoded by this gene contains a zinc finger domain in the C terminus of the coding region. [provided by RefSeq, Jul 2009] |
UniProt Code: | Q15022 |
NCBI GenInfo Identifier: | 116242808 |
NCBI Gene ID: | 23512 |
NCBI Accession: | Q15022.3 |
UniProt Secondary Accession: | Q15022,Q96BD9, |
UniProt Related Accession: | Q15022 |
Molecular Weight: | 739 |
NCBI Full Name: | Polycomb protein SUZ12 |
NCBI Synonym Full Names: | SUZ12 polycomb repressive complex 2 subunit |
NCBI Official Symbol: | SUZ12 |
NCBI Official Synonym Symbols: | CHET9; JJAZ1 |
NCBI Protein Information: | polycomb protein SUZ12; chET 9 protein; joined to JAZF1 protein; suppressor of zeste 12 homolog; suppressor of zeste 12 protein homolog; chromatin precipitated E2F target 9 protein |
UniProt Protein Name: | Polycomb protein SUZ12 |
UniProt Synonym Protein Names: | Chromatin precipitated E2F target 9 protein; ChET 9 protein; Joined to JAZF1 protein; Suppressor of zeste 12 protein homolog |
Protein Family: | Polycomb protein |
UniProt Gene Name: | SUZ12 |
UniProt Entry Name: | SUZ12_HUMAN |