SUZ12 Antibody (PACO46538)
- SKU:
- PACO46538
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Application:
- ELISA
- Application:
- WB
- Application:
- IHC
- Application:
- IF
- Antibody type:
- Polyclonal
- Conjugation:
- Unconjugated
Description
抗体名: | SUZ12 Antibody (PACO46538) |
抗体コード: | PACO46538 |
サイズ: | 50ug |
宿主種: | Rabbit |
申し込み: | ELISA, WB, IHC, IF |
推奨される希釈: | ELISA:1:2000-1:10000, WB:1:500-1:5000, IHC:1:1000-1:2000, IF:1:50-1:500 |
反応性: | Human, Rat |
免疫原: | Recombinant Human Polycomb protein SUZ12 protein (131-305AA) |
憲法: | Liquid |
ストレージバッファ: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
精製方法: | >95%, Protein G purified |
抗体のクローン性: | Polyclonal |
アイソタイプ: | IgG |
Conjugate: | Non-conjugated |
Western Blot. Positive WB detected in: Rat kidney tissue, Rat liver tissue. All lanes: SUZ12 antibody at 3.4µg/ml. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 84 kDa. Observed band size: 84 kDa.. | |
Immunofluorescence staining of HepG2 cells with PACO46538 at 1:333, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). | |
IHC image of PACO46538 diluted at 1:1000 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
バックグラウンド: | Polycomb group (PcG) protein. Component of the PRC2/EED-EZH2 complex, which methylates 'Lys-9' (H3K9me) and 'Lys-27' (H3K27me) of histone H3, leading to transcriptional repression of the affected target gene. The PRC2/EED-EZH2 complex may also serve as a recruiting platform for DNA methyltransferases, thereby linking two epigenetic repression systems. Genes repressed by the PRC2/EED-EZH2 complex include HOXC8, HOXA9, MYT1 and CDKN2A. |
シノニム: | Polycomb protein SUZ12 (Chromatin precipitated E2F target 9 protein) (ChET 9 protein) (Joined to JAZF1 protein) (Suppressor of zeste 12 protein homolog), SUZ12, CHET9 JJAZ1 KIAA0160 |
UniProt Protein Function: | SUZ12: a zinc finger protein. A breakpoint translocation of the cognate gene with JAZF1 has been reported in endometrial stromal sarcoma. |
UniProt Protein Details: | Protein type:Transcription factor; Oncoprotein; C2H2-type zinc finger protein Chromosomal Location of Human Ortholog: 17q11.2 Cellular Component: nucleoplasm; ESC/E(Z) complex; sex chromatin; nucleolus; nucleus Molecular Function:histone methyltransferase activity; protein binding; sequence-specific DNA binding; RNA binding; metal ion binding; chromatin binding; methylated histone residue binding Biological Process: histone methylation; negative regulation of cell differentiation; transcription, DNA-dependent; negative regulation of gene expression, epigenetic; positive regulation of cell proliferation; histone ubiquitination; gene expression; negative regulation of transcription from RNA polymerase II promoter; regulation of gene expression, epigenetic |
NCBI Summary: | This zinc finger gene has been identified at the breakpoints of a recurrent chromosomal translocation reported in endometrial stromal sarcoma. Recombination of these breakpoints results in the fusion of this gene and JAZF1. The protein encoded by this gene contains a zinc finger domain in the C terminus of the coding region. [provided by RefSeq, Jul 2009] |
UniProt Code: | Q15022 |
NCBI GenInfo Identifier: | 116242808 |
NCBI Gene ID: | 23512 |
NCBI Accession: | Q15022.3 |
UniProt Secondary Accession: | Q15022,Q96BD9, |
UniProt Related Accession: | Q15022 |
Molecular Weight: | 739 |
NCBI Full Name: | Polycomb protein SUZ12 |
NCBI Synonym Full Names: | SUZ12 polycomb repressive complex 2 subunit |
NCBI Official Symbol: | SUZ12 |
NCBI Official Synonym Symbols: | CHET9; JJAZ1 |
NCBI Protein Information: | polycomb protein SUZ12; chET 9 protein; joined to JAZF1 protein; suppressor of zeste 12 homolog; suppressor of zeste 12 protein homolog; chromatin precipitated E2F target 9 protein |
UniProt Protein Name: | Polycomb protein SUZ12 |
UniProt Synonym Protein Names: | Chromatin precipitated E2F target 9 protein; ChET 9 protein; Joined to JAZF1 protein; Suppressor of zeste 12 protein homolog |
Protein Family: | Polycomb protein |
UniProt Gene Name: | SUZ12 |
UniProt Entry Name: | SUZ12_HUMAN |
Antibodies |
Anti-SUZ12 Antibody (CAB7786)[KO Validated] |
Anti-SUZ12 Antibody (CAB4348) |
SUZ12 Antibody (PACO12592) |
Secondary Antibody |
Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014) |
Recommended Products |
Anti-FITC Goat Anti-Rabbit IgG (H+L) Antibody (CABS011) |
Anti-HRP-conjugated Beta Actin Antibody (CABC028) |