Anti-SF3B1 Antibody (CAB15801)
- SKU:
- CAB15801
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Epigenetics and Nuclear Signaling
Frequently bought together:
Description
抗体名: | Anti-SF3B1 Antibody |
抗体コード: | CAB15801 |
抗体サイズ: | 20uL, 50uL, 100uL |
申し込み: | WB IF |
反応性: | Human, Mouse, Rat |
宿主種: | Rabbit |
免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-144 of human SF3B1 (NP_001005526.1). |
申し込み: | WB IF |
推奨希釈: | WB 1:500 - 1:2000 IF 1:50 - 1:200 |
反応性: | Human, Mouse, Rat |
ポジティブサンプル: | U-87MG, Jurkat, HeLa, NIH/3T3, Mouse brain, Mouse liver |
免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-144 of human SF3B1 (NP_001005526.1). |
精製方法: | Affinity purification |
ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
アイソタイプ: | IgG |
順序: | MAKI AKTH EDIE AQIR EIQG KKAA LDEA QGVG LDST GYYD QEIY GGSD SRFA GYVT SIAA TELE DDDD DYSS STSL LGQK KPGY HAPV ALLN DIPQ STEQ YDPF AEHR PPKI ADRE DEYK KHRR TMII SPER LDPF ADGF YSAA |
遺伝子ID: | 23451 |
Uniprot: | O75533 |
セルラーロケーション: | Nucleus speckle |
計算された分子量: | 16kDa/145kDa |
観察された分子量: | 160kDa |
同義語: | SF3B1, Hsh155, MDS, PRP10, PRPF10, SAP155, SF3b155 |
バックグラウンド: | This gene encodes subunit 1 of the splicing factor 3b protein complex. Splicing factor 3b, together with splicing factor 3a and a 12S RNA unit, forms the U2 small nuclear ribonucleoproteins complex (U2 snRNP). The splicing factor 3b/3a complex binds pre-mRNA upstream of the intron's branch site in a sequence independent manner and may anchor the U2 snRNP to the pre-mRNA. Splicing factor 3b is also a component of the minor U12-type spliceosome. The carboxy-terminal two-thirds of subunit 1 have 22 non-identical, tandem HEAT repeats that form rod-like, helical structures. Alternative splicing results in multiple transcript variants encoding different isoforms. |
UniProt Protein Function: | SF3B1: subunit of the splicing factor SF3B required for 'A' complex assembly formed by the stable binding of U2 snRNP to the branchpoint sequence (bps) in pre-mRNA. Sequence independent binding of SF3A/SF3B complex upstream of the branch site is essential, it may anchor U2 snRNP to the pre-mRNA. May also be involved in the assembly of the 'E' complex. Belongs also to the minor U12-dependent spliceosome, which is involved in the splicing of rare class of nuclear pre-mRNA intron. Phosphorylated during the splicing catalytic steps. |
UniProt Protein Details: | Protein type:RNA-binding; Spliceosome; RNA splicing Chromosomal Location of Human Ortholog: 2q33.1 Cellular Component: nucleoplasm; nucleus; snRNP U2; U12-dependent spliceosome Molecular Function:mRNA binding; protein binding Biological Process: nuclear mRNA splicing, via spliceosome; positive regulation of gene expression, epigenetic; spliceosome assembly Disease: Myelodysplastic Syndrome |
NCBI Summary: | This gene encodes subunit 1 of the splicing factor 3b protein complex. Splicing factor 3b, together with splicing factor 3a and a 12S RNA unit, forms the U2 small nuclear ribonucleoproteins complex (U2 snRNP). The splicing factor 3b/3a complex binds pre-mRNA upstream of the intron's branch site in a sequence independent manner and may anchor the U2 snRNP to the pre-mRNA. Splicing factor 3b is also a component of the minor U12-type spliceosome. The carboxy-terminal two-thirds of subunit 1 have 22 non-identical, tandem HEAT repeats that form rod-like, helical structures. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008] |
UniProt Code: | O75533 |
NCBI GenInfo Identifier: | 269849656 |
NCBI Gene ID: | 23451 |
NCBI Accession: | O75533.3 |
UniProt Secondary Accession: | O75533,E9PCH3, |
UniProt Related Accession: | O75533 |
Molecular Weight: | 16,020 Da |
NCBI Full Name: | Splicing factor 3B subunit 1 |
NCBI Synonym Full Names: | splicing factor 3b subunit 1 |
NCBI Official Symbol: | SF3B1 |
NCBI Official Synonym Symbols: | MDS; PRP10; Hsh155; PRPF10; SAP155; SF3b155 |
NCBI Protein Information: | splicing factor 3B subunit 1 |
UniProt Protein Name: | Splicing factor 3B subunit 1 |
UniProt Synonym Protein Names: | Pre-mRNA-splicing factor SF3b 155 kDa subunit; SF3b155; Spliceosome-associated protein 155; SAP 155 |
Protein Family: | Splicing factor |
UniProt Gene Name: | SF3B1 |
UniProt Entry Name: | SF3B1_HUMAN |
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