Anti-RNF40 抗体 (CAB6443)

抗体名:	Anti-RNF40 Antibody
抗体コード:	CAB6443
抗体サイズ:	20uL, 50uL, 100uL
申し込み:	WB IHC IF IP ChIP
反応性:	Human
宿主種:	Rabbit
免疫原:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human RNF40 (NP_055586.1).

申し込み:	WB IHC IF IP ChIP
推奨希釈:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:20 - 1:100 ChIP 1:50 - 1:200
反応性:	Human
ポジティブサンプル:	MCF7, HepG2, U-937

免疫原:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human RNF40 (NP_055586.1).
精製方法:	Affinity purification
ストレージバッファ:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
アイソタイプ:	IgG
順序:	MSGP GNKR AAGD GGSG PPEK KLSR EEKT TTTL IEPI RLGG ISST EEMD LKVL QFKN KKLA ERLE QRQA CEDE LRER IEKL EKRQ ATDD ATLL IVNR YWAQ LDET VEAL LRCH ESQG ELSS APEA PGTQ EGPT CDGT PLPE PGTS ELRD PLLM QLRP PLSE PALA FVVA LGAS SSEE VELE LQGR MEFS KAAV SRVV EASD RLQR RVEE LCQR VYSR GDSE PLSE AAQA HTRE LGRE NRRL QDLA TQLQ EKHH RISL EYSE LQDK VTSA ETKV LEME TTVE DLQW DIEK LRKR EQKL NKHL
遺伝子ID:	9810
Uniprot:	O75150
セルラーロケーション:	Nucleus
計算された分子量:	94kDa/102kDa/113kDa
観察された分子量:	140kDa

同義語:

RNF40, BRE1B, RBP95, STARING

バックグラウンド:

The protein encoded by this gene contains a RING finger, a motif known to be involved in protein-protein and protein-DNA interactions. This protein was reported to interact with the tumor suppressor protein RB1. Studies of the rat counterpart suggested that this protein may function as an E3 ubiquitin-protein ligase, and facilitate the ubiquitination and degradation of syntaxin 1, which is an essential component of the neurotransmitter release machinery. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene.

UniProt Protein Function:	RNF40: Component of the RNF20/40 E3 ubiquitin-protein ligase complex that mediates monoubiquitination of 'Lys-120' of histone H2B (H2BK120ub1). H2BK120ub1 gives a specific tag for epigenetic transcriptional activation and is also prerequisite for histone H3 'Lys-4' and 'Lys-79' methylation (H3K4me and H3K79me, respectively). It thereby plays a central role in histone code and gene regulation. The RNF20/40 complex forms a H2B ubiquitin ligase complex in cooperation with the E2 enzyme UBE2A or UBE2B; reports about the cooperation with UBE2E1/UBCH are contradictory. Required for transcriptional activation of Hox genes. Component of the RNF20/40 complex (also known as BRE1 complex) probably composed of 2 copies of RNF20/BRE1A and 2 copies of RNF40/BRE1B. Interacts with UBE2E1/UBCH6. Interacts with RB1 and WAC. Ubiquitously expressed. Expressed at higher level in testis, heart and pancreas, while it is only weakly expressed in lung, skeletal muscle and small intestine. Belongs to the BRE1 family. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Ubiquitin conjugating system; Ligase; Ubiquitin ligase; EC 6.3.2.-; EC 6.3.2.19 Chromosomal Location of Human Ortholog: 16p11.2-p11.1 Cellular Component: nucleoplasm; neuron projection; membrane; nucleus; ubiquitin ligase complex Molecular Function:protein binding; protein homodimerization activity; mRNA 3'-UTR binding; zinc ion binding; ubiquitin protein ligase binding; protein complex binding; syntaxin-1 binding; ubiquitin-protein ligase activity; ligase activity Biological Process: histone monoubiquitination; ubiquitin-dependent protein catabolic process; regulation of mitotic cell cycle; histone H2B ubiquitination
NCBI Summary:	The protein encoded by this gene contains a RING finger, a motif known to be involved in protein-protein and protein-DNA interactions. This protein was reported to interact with the tumor suppressor protein RB1. Studies of the rat counterpart suggested that this protein may function as an E3 ubiquitin-protein ligase, and facilitate the ubiquitination and degradation of syntaxin 1, which is an essential component of the neurotransmitter release machinery. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2011]
UniProt Code:	O75150
NCBI GenInfo Identifier:	313104295
NCBI Gene ID:	9810
NCBI Accession:	O75150.4
UniProt Secondary Accession:	O75150,Q6AHZ6, Q6N005, Q7L3T6, Q8N615, Q96T18, Q9BSV9 Q9HC82,
UniProt Related Accession:	O75150
Molecular Weight:	1001
NCBI Full Name:	E3 ubiquitin-protein ligase BRE1B
NCBI Synonym Full Names:	ring finger protein 40, E3 ubiquitin protein ligase
NCBI Official Symbol:	RNF40
NCBI Official Synonym Symbols:	BRE1B; RBP95; STARING
NCBI Protein Information:	E3 ubiquitin-protein ligase BRE1B; BRE1-B; Rb-associated protein; BRE1 E3 ubiquitin ligase homolog B; 95 kDa retinoblastoma-associated protein; 95 kDa retinoblastoma protein binding protein
UniProt Protein Name:	E3 ubiquitin-protein ligase BRE1B
UniProt Synonym Protein Names:	95 kDa retinoblastoma-associated protein; RBP95; RING finger protein 40
Protein Family:	E3 ubiquitin-protein ligase
UniProt Gene Name:	RNF40
UniProt Entry Name:	BRE1B_HUMAN

	Western blot analysis of extracts of various cell lines, using RNF40 antibody (CAB6443) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 90s.
	Immunofluorescence analysis of U2OS cells using RNF40 antibody (CAB6443).
	Chromatin immunoprecipitation analysis of extracts of HeLa cells, using RNF40 antibody (CAB6443) and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

Antibodies

Anti-RNF40 Antibody (CAB9598)

RNF40 Antibody (PACO11875)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)