Anti-Phospho-NBN (S343) Antibody (RACO0058)
- SKU:
- RACO0058
- Product type:
- Recombinant Antibody
- Reactivity:
- Human
- Host Species:
- Human
- Isotype:
- IgG
- Application:
- WB
- Application:
- ELISA
- Conjugation:
- Unconjugated
Description
商品名: | Phospho-NBN (S343) Antibody |
Product SKU: | RACO0058 |
サイズ: | 50ul |
宿主種: | Human |
申し込み: | ELISA, WB |
推奨される希釈: | WB:1:500-1:5000 |
反応性: | Human |
免疫原: | A synthesized peptide derived from human Phospho-NBN (S343) |
憲法: | Liquid |
ストレージバッファ: | Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
精製方法: | Affinity-chromatography |
抗体のクローン性: | Monoclonal |
アイソタイプ: | Rabbit IgG |
Conjugate: | Non-conjugated |
バックグラウンド: | Component of the MRE11-RAD50-NBN (MRN complex) which plays a critical role in the cellular response to DNA damage and the maintenance of chromosome integrity. The complex is involved in double-strand break (DSB) repair, DNA recombination, maintenance of telomere integrity, cell cycle checkpoint control and meiosis. The complex possesses single-strand endonuclease activity and double-strand-specific 3'-5' exonuclease activity, which are provided by MRE11. RAD50 may be required to bind DNA ends and hold them in close proximity. NBN modulate the DNA damage signal sensing by recruiting PI3/PI4-kinase family members ATM, ATR, and probably DNA-PKcs to the DNA damage sites and activating their functions. It can also recruit MRE11 and RAD50 to the proximity of DSBs by an interaction with the histone H2AX. NBN also functions in telomere length maintenance by generating the 3' overhang which serves as a primer for telomerase dependent telomere elongation. NBN is a major player in the control of intra-S-phase checkpoint and there is some evidence that NBN is involved in G1 and G2 checkpoints. The roles of NBS1/MRN encompass DNA damage sensor, signal transducer, and effector, which enable cells to maintain DNA integrity and genomic stability. Forms a complex with RBBP8 to link DNA double-strand break sensing to resection. Enhances AKT1 phosphorylation possibly by association with the mTORC2 complex. |
シノニム: | Nibrin, Cell cycle regulatory protein p95, Nijmegen breakage syndrome protein 1, NBN, NBS, NBS1, P95 |
Western Blot. Positive WB detected in Hela whole cell lysate HepG2 whole cell lysate All lanes Phospho-NBN antibody at 1.98µg/ml. Secondary. Goat polyclonal to rabbit IgG at 1:50000 dilution. Predicted band size: 95 KDa. Observed band size: 95 KDa. |
UniProt Protein Function: | NBS1: a member of the MRE11/RAD50 double-strand break repair complex. Involved in DNA double-strand break repair and DNA damage-induced checkpoint activation. Mutation results in the Nijmegen breakage syndrome (NBS), an autosomal recessive chromosomal instability syndrome. |
UniProt Protein Details: | Protein type:Cell cycle regulation; DNA repair, damage Chromosomal Location of Human Ortholog: 8q21 Cellular Component: cytosol; Mre11 complex; nuclear chromosome, telomeric region; nuclear inclusion body; nucleolus; nucleoplasm; nucleus; PML body; replication fork Molecular Function:ATP-dependent DNA helicase activity; damaged DNA binding; protein binding; protein N-terminus binding; transcription factor binding Biological Process: blastocyst growth; cell cycle arrest; cell proliferation; DNA damage checkpoint; DNA damage response, signal transduction by p53 class mediator; DNA duplex unwinding; DNA repair; double-strand break repair; double-strand break repair via homologous recombination; double-strand break repair via nonhomologous end joining; double-strand break repair via synthesis-dependent strand annealing; isotype switching; meiotic cell cycle; mitotic cell cycle checkpoint; mitotic cell cycle G2/M transition DNA damage checkpoint; neuromuscular process controlling balance; positive regulation of kinase activity; positive regulation of protein amino acid autophosphorylation; positive regulation of telomere maintenance; regulation of DNA replication initiation; telomere maintenance; telomeric 3' overhang formation Disease: Aplastic Anemia; Leukemia, Acute Lymphoblastic; Nijmegen Breakage Syndrome |
NCBI Summary: | Mutations in this gene are associated with Nijmegen breakage syndrome, an autosomal recessive chromosomal instability syndrome characterized by microcephaly, growth retardation, immunodeficiency, and cancer predisposition. The encoded protein is a member of the MRE11/RAD50 double-strand break repair complex which consists of 5 proteins. This gene product is thought to be involved in DNA double-strand break repair and DNA damage-induced checkpoint activation. [provided by RefSeq, Jul 2008] |
UniProt Code: | O60934 |
NCBI GenInfo Identifier: | 74762960 |
NCBI Gene ID: | 4683 |
NCBI Accession: | O60934.1 |
UniProt Secondary Accession: | O60934,O60672, Q32NF7, Q53FM6, Q63HR6, Q7LDM2, B2R626 B2RNC5, |
UniProt Related Accession: | O60934 |
Molecular Weight: | 84,959 Da |
NCBI Full Name: | Nibrin |
NCBI Synonym Full Names: | nibrin |
NCBI Official Symbol: | NBN |
NCBI Official Synonym Symbols: | ATV; NBS; P95; NBS1; AT-V1; AT-V2 |
NCBI Protein Information: | nibrin |
UniProt Protein Name: | Nibrin |
UniProt Synonym Protein Names: | Cell cycle regulatory protein p95; Nijmegen breakage syndrome protein 1 |
Protein Family: | Nibrin |
UniProt Gene Name: | NBN |
UniProt Entry Name: | NBN_HUMAN |