Anti-Phospho-I kappa B alpha-S32/36 Antibody (CABP0614)

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SKU:
CABP0614
Product type:
Antibody
Application:
WB
Reactivity:
Human
Reactivity:
Mouse
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Immunology
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Description

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抗体名:Anti-Phospho-I kappa B alpha-S32/36 Antibody
抗体コード:CABP0614
抗体サイズ:20uL, 50uL, 100uL
申し込み:WB
反応性:Human, Mouse
宿主種:Rabbit
免疫原:A synthetic phosphorylated peptide around S32 & S36 of human I?BAlpha (NP_065390.1).
申し込み:WB
推奨希釈:WB 1:500 - 1:1000
反応性:Human, Mouse
ポジティブサンプル:HeLa, 293
免疫原:A synthetic phosphorylated peptide around S32 & S36 of human I?BAlpha (NP_065390.1).
精製方法:Affinity purification
ストレージバッファ:Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
アイソタイプ:IgG
順序:SGLD S
遺伝子ID:4792
Uniprot:P25963
セルラーロケーション:Cytoplasm, Nucleus
計算された分子量:35kDa
観察された分子量:36kDa
Anti-Phospho-I kappa B alpha-S32/36 Antibody (CABP0614)Western blot analysis of extracts of HeLa and 293 cells, using Phospho-IkBalpha-S32/36 antibody (CABP0614) at 1:1000 dilution. HeLa cells were treated by TNF-a (20ng/mL) for 30 minutes. 293 cells were treated by TNF-a (20ng/mL) for 30 minutes. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA
同義語:IKBA, MAD-3, NFKBI, IKB alpha, NFKBIA, EDAID2
バックグラウンド:This gene encodes a member of the NF-kappa-B inhibitor family, which contain multiple ankrin repeat domains. The encoded protein interacts with REL dimers to inhibit NF-kappa-B/REL complexes which are involved in inflammatory responses. The encoded protein moves between the cytoplasm and the nucleus via a nuclear localization signal and CRM1-mediated nuclear export. Mutations in this gene have been found in ectodermal dysplasia anhidrotic with T-cell immunodeficiency autosomal dominant disease.
UniProt Protein Function:Inhibits the activity of dimeric NF-kappa-B/REL complexes by trapping REL dimers in the cytoplasm through masking of their nuclear localization signals. On cellular stimulation by immune and proinflammatory responses, becomes phosphorylated promoting ubiquitination and degradation, enabling the dimeric RELA to translocate to the nucleus and activate transcription.
NCBI Summary:This gene encodes a member of the NF-kappa-B inhibitor family, which contain multiple ankrin repeat domains. The encoded protein interacts with REL dimers to inhibit NF-kappa-B/REL complexes which are involved in inflammatory responses. The encoded protein moves between the cytoplasm and the nucleus via a nuclear localization signal and CRM1-mediated nuclear export. Mutations in this gene have been found in ectodermal dysplasia anhidrotic with T-cell immunodeficiency autosomal dominant disease. [provided by RefSeq, Aug 2011]
UniProt Code:P25963
NCBI GenInfo Identifier:126682
NCBI Gene ID:4792
NCBI Accession:P25963.1
UniProt Secondary Accession:P25963,B2R8L6,
UniProt Related Accession:P25963
Molecular Weight:35,609 Da
NCBI Full Name:NF-kappa-B inhibitor alpha
NCBI Synonym Full Names:NFKB inhibitor alpha
NCBI Official Symbol:NFKBIA  
NCBI Official Synonym Symbols:IKBA; MAD-3; NFKBI  
NCBI Protein Information:NF-kappa-B inhibitor alpha
UniProt Protein Name:NF-kappa-B inhibitor alpha
UniProt Synonym Protein Names:I-kappa-B-alpha; IkB-alpha; IkappaBalpha; Major histocompatibility complex enhancer-binding protein MAD3
Protein Family:NF-kappa-B inhibitor
UniProt Gene Name:NFKBIA  
UniProt Entry Name:IKBA_HUMAN
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