Anti-PHD3 Antibody (CAB0851)

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SKU:
CAB0851
Product type:
Antibody
Antibody Type:
Monoclonal Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Synonyms:
EGLN3
Synonyms:
HIFP4H3
Synonyms:
HIFPH3
Synonyms:
PHD3
Synonyms:
egl nine homolog 3
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Description

抗体名:PHD3 Rabbit mAb
抗体コード:CAB0851
サイズ:20uL, 50uL, 100uL
同義語:EGLN3, HIFP4H3, HIFPH3, PHD3, egl nine homolog 3
申し込み:WB
反応性:Human, Mouse, Rat
宿主種:Rabbit
免疫原:A synthesized peptide derived from human PHD3.
申し込み:WB
推奨される希釈:WB 1:500 - 1:2000
反応性:Human, Mouse, Rat
ポジティブサンプル:PC-12, A-549, Mouse brain, Rat stomach
免疫原:A synthesized peptide derived from human PHD3.
精製方法:Affinity purification
保管所:Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
アイソタイプ:IgG
順序:Email for sequence
遺伝子ID:112399
Uniprot:Q9H6Z9
セルラーロケーション:Cytoplasm, Nucleus
計算された分子量:27kDa
観察された分子量:27KDa
UniProt Protein Function:EGLN3: Cellular oxygen sensor that catalyzes, under normoxic conditions, the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. Hydroxylates a specific proline found in each of the oxygen-dependent degradation (ODD) domains (N-terminal, NODD, and C-terminal, CODD) of HIF1A. Also hydroxylates HIF2A. Has a preference for the CODD site for both HIF1A and HIF2A. Hydroxylation on the NODD site by EGLN3 appears to require prior hydroxylation on the CODD site. Hydroxylated HIFs are then targeted for proteasomal degradation via the von Hippel-Lindau ubiquitination complex. Under hypoxic conditions, the hydroxylation reaction is attenuated allowing HIFs to escape degradation resulting in their translocation to the nucleus, heterodimerization with HIF1B, and increased expression of hypoxy-inducible genes. EGLN3 is the most important isozyme in limiting physiological activation of HIFs (particularly HIF2A) in hypoxia. Also hydroxylates PKM in hypoxia, limiting glycolysis. Under normoxia, hydroxylates and regulates the stability of ADRB2. Regulator of cardiomyocyte and neuronal apoptosis. In cardiomyocytes, inhibits the anti-apoptotic effect of BCL2 by disrupting the BAX-BCL2 complex. In neurons, has a NGF-induced proapoptotic effect, probably through regulating CASP3 activity. Also essential for hypoxic regulation of neutrophilic inflammation.
UniProt Protein Details:

Protein type:Oxidoreductase; EC 1.14.11.29

Chromosomal Location of Human Ortholog: 14q13.1

Cellular Component: nucleoplasm; cytoplasm; nucleus; cytosol

Molecular Function:protein binding; L-ascorbic acid binding; iron ion binding; oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, 2-oxoglutarate as one donor, and incorporation of one atom each of oxygen into both donors; peptidyl-proline 4-dioxygenase activity

Biological Process: caspase activation; apoptosis; regulation of neuron apoptosis; response to hypoxia; protein amino acid hydroxylation; peptidyl-proline hydroxylation to 4-hydroxy-L-proline; response to DNA damage stimulus; regulation of cell proliferation

UniProt Code:Q9H6Z9
NCBI GenInfo Identifier:32129515
NCBI Gene ID:112399
NCBI Accession:Q9H6Z9.1
UniProt Related Accession:Q9H6Z9
Molecular Weight:Observed: 28 kDaPredicted: 28 kDa
NCBI Full Name:Egl nine homolog 3
NCBI Synonym Full Names:egl-9 family hypoxia inducible factor 3
NCBI Official Symbol:EGLN3  
NCBI Official Synonym Symbols:PHD3; HIFPH3; HIFP4H3  
NCBI Protein Information:egl nine homolog 3
UniProt Protein Name:Egl nine homolog 3
UniProt Synonym Protein Names:HPH-1; Hypoxia-inducible factor prolyl hydroxylase 3; HIF-PH3; HIF-prolyl hydroxylase 3; HPH-3; Prolyl hydroxylase domain-containing protein 3; PHD3
UniProt Gene Name:EGLN3  
UniProt Entry Name:EGLN3_HUMAN
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