Anti-KIF13A 抗体 (CAB15880)

抗体名:	Anti-KIF13A Antibody
抗体コード:	CAB15880
抗体サイズ:	20uL, 50uL, 100uL
申し込み:	WB IF
反応性:	Human, Mouse
宿主種:	Rabbit
免疫原:	Recombinant fusion protein containing a sequence corresponding to amino acids 1300-1600 of human KIF13A (NP_001099036.1).

申し込み:	WB IF
推奨希釈:	WB 1:500 - 1:2000 IF 1:50 - 1:200
反応性:	Human, Mouse
ポジティブサンプル:	LO2

免疫原:	Recombinant fusion protein containing a sequence corresponding to amino acids 1300-1600 of human KIF13A (NP_001099036.1).
精製方法:	Affinity purification
ストレージバッファ:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
アイソタイプ:	IgG
順序:	SCGV TYEI VSNI PKAT EEIE DRET LALL AARS ENEG TSDG ETYI EKYT RGVL QVEN ILSL ERLR QAVT VKEA LSTK ARHI RRSL STPN VHNV SSSR PDLS GFDE DDKG WPEN QLDM SDYS SSYQ DVAC YGTL PRDS PRRN KEGC TSET PHAL TVSP FKAF SPQP PKFF KPLM PVKE EHKK RIAL EARP LLSQ EDSE EEEN ELEA INRK LISS QPYV PVEF ADFS VYNA SLEN REWF SSKV DLSN SRVL EKEV SRSP TTSS ITSG YFSH SASN ATLS DMVV PSSD SSDQ LAIQ TKDA DSTE H
遺伝子ID:	63971
Uniprot:	Q9H1H9
セルラーロケーション:	Cytoplasm, Endosome membrane, Golgi apparatus membrane, Midbody, centrosome, cytoskeleton, microtubule organizing center
計算された分子量:	7kDa/196kDa/197kDa/198kDa/202kDa
観察された分子量:	250kDa

同義語:

KIF13A, RBKIN, bA500C11.2

バックグラウンド:

This gene encodes a member of the kinesin family of microtubule-based motor proteins that function in the positioning of endosomes. This family member can direct mannose-6-phosphate receptor-containing vesicles from the trans-Golgi network to the plasma membrane, and it is necessary for the steady-state distribution of late endosomes/lysosomes. It is also required for the translocation of FYVE-CENT and TTC19 from the centrosome to the midbody during cytokinesis, and it plays a role in melanosome maturation. Alternative splicing of this gene results in multiple transcript variants.

UniProt Protein Function:	KIF13A: Plus end-directed microtubule-dependent motor protein involved in intracellular transport and regulating various processes such as mannose-6-phosphate receptor (M6PR) transport to the plasma membrane, endosomal sorting during melanosome biogenesis and cytokinesis. Mediates the transport of M6PR- containing vesicles from trans-Golgi network to the plasma membrane via direct interaction with the AP-1 complex. During melanosome maturation, required for delivering melanogenic enzymes from recycling endosomes to nascent melanosomes by creating peripheral recycling endosomal subdomains in melanocytes. Also required for the abcission step in cytokinesis: mediates translocation of ZFYVE26, and possibly TTC19, to the midbody during cytokinesis. Belongs to the kinesin-like protein family. 4 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Microtubule-binding; Motor Chromosomal Location of Human Ortholog: 6p23 Cellular Component: Golgi membrane; kinesin complex; microtubule; centrosome; trans-Golgi network membrane; endosome membrane; midbody Molecular Function:protein binding; microtubule binding; ATPase activity; microtubule motor activity; ATP binding Biological Process: intracellular protein transport; melanosome organization and biogenesis; metabolic process; Golgi to plasma membrane protein transport; cytokinesis; endosome to lysosome transport; microtubule-based movement
NCBI Summary:	This gene encodes a member of the kinesin family of microtubule-based motor proteins that function in the positioning of endosomes. This family member can direct mannose-6-phosphate receptor-containing vesicles from the trans-Golgi network to the plasma membrane, and it is necessary for the steady-state distribution of late endosomes/lysosomes. It is also required for the translocation of FYVE-CENT and TTC19 from the centrosome to the midbody during cytokinesis, and it plays a role in melanosome maturation. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Aug 2011]
UniProt Code:	Q9H1H9
NCBI GenInfo Identifier:	157738625
NCBI Gene ID:	63971
NCBI Accession:	NP_001099036.1
UniProt Secondary Accession:	Q9H1H9,Q5THQ2, Q5THQ3, Q9H193, Q9H194, Q9H1H8, A0JP21 A0JP22, F2Z382,
UniProt Related Accession:	Q9H1H9
Molecular Weight:	7,726 Da
NCBI Full Name:	kinesin-like protein KIF13A isoform b
NCBI Synonym Full Names:	kinesin family member 13A
NCBI Official Symbol:	KIF13A
NCBI Official Synonym Symbols:	RBKIN; bA500C11.2
NCBI Protein Information:	kinesin-like protein KIF13A
UniProt Protein Name:	Kinesin-like protein KIF13A
UniProt Synonym Protein Names:	Kinesin-like protein RBKIN
Protein Family:	Kinesin-like protein
UniProt Gene Name:	KIF13A
UniProt Entry Name:	KI13A_HUMAN

	Western blot analysis of extracts of LO2 cells, using KIF13A antibody (CAB15880) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
	Immunofluorescence analysis of HeLa cells using KIF13A antibody (CAB15880) at dilution of 1:100. Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of L929 cells using KIF13A antibody (CAB15880) at dilution of 1:100. Blue: DAPI for nuclear staining.

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)