Anti-GZMA 抗体 (CAB6231)

抗体名:	Anti-GZMA Antibody
抗体コード:	CAB6231
抗体サイズ:	20uL, 50uL, 100uL
申し込み:	WB IF
反応性:	Human, Mouse, Rat
宿主種:	Rabbit
免疫原:	Recombinant fusion protein containing a sequence corresponding to amino acids 29-262 of human GZMA (NP_006135.1).

申し込み:	WB IF
推奨希釈:	WB 1:500 - 1:2000 IF 1:50 - 1:200
反応性:	Human, Mouse, Rat
ポジティブサンプル:	Mouse spleen, Mouse thymus, Rat intestine, Rat spleen

免疫原:	Recombinant fusion protein containing a sequence corresponding to amino acids 29-262 of human GZMA (NP_006135.1).
精製方法:	Affinity purification
ストレージバッファ:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
アイソタイプ:	IgG
順序:	IIGG NEVT PHSR PYMV LLSL DRKT ICAG ALIA KDWV LTAA HCNL NKRS QVIL GAHS ITRE EPTK QIML VKKE FPYP CYDP ATRE GDLK LLQL TEKA KINK YVTI LHLP KKGD DVKP GTMC QVAG WGRT HNSA SWSD TLRE VNIT IIDR KVCN DRNH YNFN PVIG MNMV CAGS LRGG RDSC NGDS GSPL LCEG VFRG VTSF GLEN KCGD PRGP GVYI LLSK KHLN WIIM TIKG AV
遺伝子ID:	3001
Uniprot:	P12544
セルラーロケーション:	Cytoplasmic granule, Secreted
計算された分子量:	27kDa/28kDa
観察された分子量:	35kDa

同義語:

GZMA, CTLA3, HFSP

バックグラウンド:

Cytolytic T lymphocytes (CTL) and natural killer (NK) cells share the remarkable ability to recognize, bind, and lyse specific target cells. They are thought to protect their host by lysing cells bearing on their surface 'nonself' antigens, usually peptides or proteins resulting from infection by intracellular pathogens. The protein described here is a T cell- and natural killer cell-specific serine protease that may function as a common component necessary for lysis of target cells by cytotoxic T lymphocytes and natural killer cells.

UniProt Protein Function:	GZMA: This enzyme is necessary for target cell lysis in cell- mediated immune responses. It cleaves after Lys or Arg. Cleaves APEX1 after 'Lys-31' and destroys its oxidative repair activity. Involved in apoptosis. Homodimer; disulfide-linked. Interacts with APEX1. Dexamethasone (DEX) induces expression of isoform beta and represses expression of isoform alpha. The alteration in expression is mediated by binding of glucocorticoid receptor to independent promoters adjacent to the alternative first exons of isoform alpha and isoform beta. Belongs to the peptidase S1 family. Granzyme subfamily. 2 isoforms of the human protein are produced by alternative promoter.
UniProt Protein Details:	Protein type:Secreted, signal peptide; Protease; EC 3.4.21.78; Apoptosis; Secreted Chromosomal Location of Human Ortholog: 5q11-q12 Cellular Component: extracellular region; immunological synapse; nucleus Molecular Function:protein binding; protein homodimerization activity; serine-type endopeptidase activity Biological Process: proteolysis involved in cellular protein catabolic process; positive regulation of apoptosis; apoptosis; cytolysis; negative regulation of oxidoreductase activity; negative regulation of endodeoxyribonuclease activity; immune response; negative regulation of DNA binding
NCBI Summary:	Cytolytic T lymphocytes (CTL) and natural killer (NK) cells share the remarkable ability to recognize, bind, and lyse specific target cells. They are thought to protect their host by lysing cells bearing on their surface 'nonself' antigens, usually peptides or proteins resulting from infection by intracellular pathogens. The protein described here is a T cell- and natural killer cell-specific serine protease that may function as a common component necessary for lysis of target cells by cytotoxic T lymphocytes and natural killer cells. [provided by RefSeq, Jul 2008]
UniProt Code:	P12544
NCBI GenInfo Identifier:	317373360
NCBI Gene ID:	3001
NCBI Accession:	P12544.2
UniProt Secondary Accession:	P12544,Q6IB36, A4PHN1,
UniProt Related Accession:	P12544
Molecular Weight:	262
NCBI Full Name:	Granzyme A
NCBI Synonym Full Names:	granzyme A (granzyme 1, cytotoxic T-lymphocyte-associated serine esterase 3)
NCBI Official Symbol:	GZMA
NCBI Official Synonym Symbols:	HFSP; CTLA3
NCBI Protein Information:	granzyme A; HF; h factor; CTL tryptase; fragmentin-1; cytotoxic T-lymphocyte proteinase 1; Granzyme A (Cytotoxic T-lymphocyte-associated serine esterase-3; Hanukah factor serine protease)
UniProt Protein Name:	Granzyme A
UniProt Synonym Protein Names:	CTL tryptase; Cytotoxic T-lymphocyte proteinase 1; Fragmentin-1; Granzyme-1; Hanukkah factor; H factor; HF
Protein Family:	Granzyme
UniProt Gene Name:	GZMA
UniProt Entry Name:	GRAA_HUMAN

	Western blot analysis of extracts of various cell lines, using GZMA antibody (CAB6231) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunofluorescence analysis of U2OS cells using GZMA antibody (CAB6231) at dilution of 1:100. Blue: DAPI for nuclear staining.

Antibodies	ELISA Kits
GZMA Antibody (PACO04022)	Human Granzyme A (GZMA) ELISA Kit
GZMA Antibody (PACO09654)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)