Anti-GRK5 Antibody (CAB3899)
- SKU:
- CAB3899
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Cell Death
Description
抗体名: | Anti-GRK5 Antibody |
抗体コード: | CAB3899 |
抗体サイズ: | 20uL, 50uL, 100uL |
申し込み: | WB IHC IF |
反応性: | Human, Mouse, Rat |
宿主種: | Rabbit |
免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 371-590 of human GRK5 (NP_005299.1). |
申し込み: | WB IHC IF |
推奨希釈: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 |
反応性: | Human, Mouse, Rat |
ポジティブサンプル: | A-549, U-251MG, HepG2, 293T, HeLa, HT-1080, Mouse liver |
免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 371-590 of human GRK5 (NP_005299.1). |
精製方法: | Affinity purification |
ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
アイソタイプ: | IgG |
順序: | LGCL IYEM IEGQ SPFR GRKE KVKR EEVD RRVL ETEE VYSH KFSE EAKS ICKM LLTK DAKQ RLGC QEEG AAEV KRHP FFRN MNFK RLEA GMLD PPFV PDPR AVYC KDVL DIEQ FSTV KGVN LDHT DDDF YSKF STGS VSIP WQNE MIET ECFK ELNV FGPN GTLP PDLN RNHP PEPP KKGL LQRL FKRQ HQNN SKSS PSSK TSFN HHIN SNHV SSNS TGSS |
遺伝子ID: | 2869 |
Uniprot: | P34947 |
セルラーロケーション: | Cell membrane, Cytoplasm, Nucleus, Peripheral membrane protein |
計算された分子量: | 68kDa |
観察された分子量: | 68kDa |
同義語: | GRK5, GPRK5 |
バックグラウンド: | This gene encodes a member of the guanine nucleotide-binding protein (G protein)-coupled receptor kinase subfamily of the Ser/Thr protein kinase family. The protein phosphorylates the activated forms of G protein-coupled receptors thus initiating their deactivation. It has also been shown to play a role in regulating the motility of polymorphonuclear leukocytes (PMNs). |
UniProt Protein Function: | GRK5: Serine/threonine kinase that phosphorylates preferentially the activated forms of a variety of G-protein- coupled receptors (GPCRs). Such receptor phosphorylation initiates beta-arrestin-mediated receptor desensitization, internalization, and signaling events leading to their down-regulation. Phosphorylates a variety of GPCRs, including adrenergic receptors, muscarinic acetylcholine receptors (more specifically Gi-coupled M2/M4 subtypes), dopamine receptors and opioid receptors. In addition to GPCRs, also phosphorylates various substrates: Hsc70- interacting protein/ST13, TP53/p53, HDAC5, and arrestin-1/ARRB1. Phosphorylation of ARRB1 by GRK5 inhibits G-protein independent MAPK1/MAPK3 signaling downstream of 5HT4-receptors. Phosphorylation of HDAC5, a repressor of myocyte enhancer factor 2 (MEF2) leading to nuclear export of HDAC5 and allowing MEF2- mediated transcription. Phosphorylation of TP53/p53, a crucial tumor suppressor, inhibits TP53/p53-mediated apoptosis. Phosphorylation of ST13 regulates internalization of the chemokine receptor. Phosphorylates rhodopsin (RHO) (in vitro) and a non G- protein-coupled receptor, LRP6 during Wnt signaling (in vitro). Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. GPRK subfamily. |
UniProt Protein Details: | Protein type:Kinase, protein; Protein kinase, AGC; Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.16; AGC group; GRK family; GRK subfamily Chromosomal Location of Human Ortholog: 10q26.11 Cellular Component: cytoplasm; focal adhesion; nuclear membrane; nucleus; plasma membrane Molecular Function:beta-adrenergic receptor kinase activity; phospholipid binding; protein binding; protein kinase C binding; protein serine/threonine kinase activity Biological Process: G-protein signaling, coupled to cAMP nucleotide second messenger; negative regulation of apoptosis; positive regulation of cell proliferation; protein amino acid autophosphorylation; regulation of cell cycle; regulation of G-protein coupled receptor protein signaling pathway; tachykinin signaling pathway |
NCBI Summary: | This gene encodes a member of the guanine nucleotide-binding protein (G protein)-coupled receptor kinase subfamily of the Ser/Thr protein kinase family. The protein phosphorylates the activated forms of G protein-coupled receptors thus initiating their deactivation. It has also been shown to play a role in regulating the motility of polymorphonuclear leukocytes (PMNs). [provided by RefSeq, Jul 2008] |
UniProt Code: | P34947 |
NCBI GenInfo Identifier: | 462203 |
NCBI Gene ID: | 2869 |
NCBI Accession: | P34947.1 |
UniProt Secondary Accession: | P34947,Q5T059, D3DRD0, |
UniProt Related Accession: | P34947 |
Molecular Weight: | 67,787 Da |
NCBI Full Name: | G protein-coupled receptor kinase 5 |
NCBI Synonym Full Names: | G protein-coupled receptor kinase 5 |
NCBI Official Symbol: | GRK5 |
NCBI Official Synonym Symbols: | GPRK5 |
NCBI Protein Information: | G protein-coupled receptor kinase 5 |
UniProt Protein Name: | G protein-coupled receptor kinase 5 |
UniProt Synonym Protein Names: | G protein-coupled receptor kinase GRK5 |
Protein Family: | G protein-coupled receptor kinase |
UniProt Gene Name: | GRK5 |
UniProt Entry Name: | GRK5_HUMAN |