Anti-GLIS3 抗体 (CAB12166)

抗体名:	Anti-GLIS3 Antibody
抗体コード:	CAB12166
抗体サイズ:	20uL, 50uL, 100uL
申し込み:	WB
反応性:	Human, Mouse
宿主種:	Rabbit
免疫原:	Recombinant fusion protein containing a sequence corresponding to amino acids 606-775 of human GLIS3 (NP_689842.3).

申し込み:	WB
推奨希釈:	WB 1:500 - 1:2000
反応性:	Human, Mouse
ポジティブサンプル:	U-87MG, BxPC-3, HepG2, A-549, Mouse kidney

免疫原:	Recombinant fusion protein containing a sequence corresponding to amino acids 606-775 of human GLIS3 (NP_689842.3).
精製方法:	Affinity purification
ストレージバッファ:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
アイソタイプ:	IgG
順序:	DAGA ERFA PSAP SPHH ISPR RVPA PSSI LQRT QPPY TQQP SGSH LKSY QPET NSSF QPNG IHVH GFYG QLQK FCPP HYPD SQRI VPPV SSCS VVPS FEDC LVPT SMGQ ASFD VFHR AFST HSGI TVYD LPSS SSSL FGES LRSG AEDA TFLQ ISTV DRCP SQLS SVYT EG
遺伝子ID:	169792
Uniprot:	Q8NEA6
セルラーロケーション:	Nucleus
計算された分子量:	83kDa/99kDa
観察された分子量:	72kDa

同義語:

GLIS3, NDH, ZNF515

バックグラウンド:

This gene is a member of the GLI-similar zinc finger protein family and encodes a nuclear protein with five C2H2-type zinc finger domains. This protein functions as both a repressor and activator of transcription and is specifically involved in the development of pancreatic beta cells, the thyroid, eye, liver and kidney. Mutations in this gene have been associated with neonatal diabetes and congenital hypothyroidism (NDH). Alternatively spliced variants that encode different protein isoforms have been described but the full-length nature of only two have been determined.

UniProt Protein Function:	GLIS3: Acts as both a repressor and activator of transcription. Binds to the consensus sequence 5'-GACCACCCAC-3'. Defects in GLIS3 are a cause of diabetes mellitus neonatal with congenital hypothyroidism (NDH). NDH is a neonatal diabetes syndrome associated with congenital hypothyroidism, congenital glaucoma, hepatic fibrosis and polycystic kidneys. Belongs to the GLI C2H2-type zinc-finger protein family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:C2H2-type zinc finger protein; Transcription factor Chromosomal Location of Human Ortholog: 9p24.2 Cellular Component: nucleus Biological Process: negative regulation of transcription from RNA polymerase II promoter; positive regulation of transcription from RNA polymerase II promoter; transcription from RNA polymerase II promoter Disease: Diabetes Mellitus, Neonatal, With Congenital Hypothyroidism
NCBI Summary:	This gene is a member of the GLI-similar zinc finger protein family and encodes a nuclear protein with five C2H2-type zinc finger domains. This protein functions as both a repressor and activator of transcription and is specifically involved in the development of pancreatic beta cells, the thyroid, eye, liver and kidney. Mutations in this gene have been associated with neonatal diabetes and congenital hypothyroidism (NDH). Alternatively spliced variants that encode different protein isoforms have been described but the full-length nature of only two have been determined. [provided by RefSeq, Jul 2008]
UniProt Code:	Q8NEA6
NCBI GenInfo Identifier:	322510143
NCBI Gene ID:	169792
NCBI Accession:	Q8NEA6.5
UniProt Secondary Accession:	Q8NEA6,Q1PHK5, B1AL19,
UniProt Related Accession:	Q8NEA6
Molecular Weight:	84kDa
NCBI Full Name:	Zinc finger protein GLIS3
NCBI Synonym Full Names:	GLIS family zinc finger 3
NCBI Official Symbol:	GLIS3
NCBI Official Synonym Symbols:	NDH; ZNF515
NCBI Protein Information:	zinc finger protein GLIS3
UniProt Protein Name:	Zinc finger protein GLIS3
UniProt Synonym Protein Names:	GLI-similar 3; Zinc finger protein 515
Protein Family:	Zinc finger protein
UniProt Gene Name:	GLIS3

Western blot analysis of extracts of various cell lines, using GLIS3 antibody (CAB12166) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s.

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)