Anti-GBF1 Antibody (CAB12539)
- SKU:
- CAB12539
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Immunology
Frequently bought together:
Description
抗体名: | Anti-GBF1 Antibody |
抗体コード: | CAB12539 |
抗体サイズ: | 20uL, 50uL, 100uL |
申し込み: | WB |
反応性: | Human, Mouse |
宿主種: | Rabbit |
免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-85 of human GBF1 (NP_004184.1). |
申し込み: | WB |
推奨希釈: | WB 1:500 - 1:2000 |
反応性: | Human, Mouse |
ポジティブサンプル: | HeLa |
免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-85 of human GBF1 (NP_004184.1). |
精製方法: | Affinity purification |
ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
アイソタイプ: | IgG |
順序: | MVDK NIYI IQGE INIV VGAI KRNA RWST HTPL DEER DPLL HSFG HLKE VLNS ITEL SEIE PNVF LRPF LEVI RSED TTGP ITGL A |
遺伝子ID: | 8729 |
Uniprot: | Q92538 |
セルラーロケーション: | Cytoplasm, Endoplasmic reticulum-Golgi intermediate compartment, Golgi apparatus, Lipid droplet, Membrane, Peripheral membrane protein, cis-Golgi network, trans-Golgi network |
計算された分子量: | 206kDa |
観察された分子量: | 260kDa |
同義語: | GBF1, ARF1GEF |
バックグラウンド: | This gene encodes a member of the Sec7 domain family. The encoded protein is a guanine nucleotide exchange factor that regulates the recruitment of proteins to membranes by mediating GDP to GTP exchange. The encoded protein is localized to the Golgi apparatus and plays a role in vesicular trafficking by activating ADP ribosylation factor 1. The encoded protein has also been identified as an important host factor for viral replication. Multiple transcript variants have been observed for this gene. |
UniProt Protein Function: | Guanine-nucleotide exchange factor (GEF) for members of the Arf family of small GTPases involved in trafficking in the early secretory pathway; its GEF activity initiates the coating of nascent vesicles via the localized generation of activated ARFs through replacement of GDP with GTP. Recruitment to cis-Golgi membranes requires membrane association of Arf-GDP and can be regulated by ARF1, ARF3, ARF4 and ARF5. Involved in the recruitment of the COPI coat complex to the endoplasmic reticulum exit sites (ERES), and the endoplasmic reticulum-Golgi intermediate (ERGIC) and cis-Golgi compartments which implicates ARF1 activation. Involved in COPI vesicle-dependent retrograde transport from the ERGIC and cis-Golgi compartments to the endoplasmatic reticulum (ER) (PubMed:16926190, PubMed:17956946, PubMed:18003980, PubMed:12047556, PubMed:12808027, PubMed:19039328, PubMed:24213530). Involved in the trans-Golgi network recruitment of GGA1, GGA2, GGA3, BIG1, BIG2, and the AP-1 adaptor protein complex related to chlathrin-dependent transport; the function requires its GEF activity (probably at least in part on ARF4 and ARF5) (PubMed:23386609). Has GEF activity towards ARF1 (PubMed:15616190). Has in vitro GEF activity towards ARF5. Involved in the processing of PSAP (PubMed:17666033). Required for the assembly of the Golgi apparatus (PubMed:12808027, PubMed:18003980). The AMPK-phosphorylated form is involved in Golgi disassembly during mitotis and under stress conditions (PubMed:18063581, PubMed:23418352). May be involved in the COPI vesicle-dependent recruitment of PNPLA2 to lipid droplets; however, this function is under debate (PubMed:19461073, PubMed:22185782). In neutrophils, involved in G protein-coupled receptor (GPCR)-mediated chemotaxis und superoxide production. Proposed to be recruited by phosphatidylinositol-phosphates generated upon GPCR stimulation to the leading edge where it recruits and activates ARF1, and is involved in recruitment of GIT2 and the NADPH oxidase complex (PubMed:22573891). |
NCBI Summary: | This gene encodes a member of the Sec7 domain family. The encoded protein is a guanine nucleotide exchange factor that regulates the recruitment of proteins to membranes by mediating GDP to GTP exchange. The encoded protein is localized to the Golgi apparatus and plays a role in vesicular trafficking by activating ADP ribosylation factor 1. The encoded protein has also been identified as an important host factor for viral replication. Multiple transcript variants have been observed for this gene. [provided by RefSeq, Dec 2010] |
UniProt Code: | Q92538 |
NCBI GenInfo Identifier: | 13124260 |
NCBI Gene ID: | 8729 |
NCBI Accession: | Q92538.2 |
UniProt Secondary Accession: | Q92538,Q149P0, Q149P1, Q5VXX3, Q96CK6, Q96HZ3, Q9H473 |
UniProt Related Accession: | Q92538 |
Molecular Weight: | 206kDa |
NCBI Full Name: | Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1 |
NCBI Synonym Full Names: | golgi brefeldin A resistant guanine nucleotide exchange factor 1 |
NCBI Official Symbol: | GBF1 |
NCBI Official Synonym Symbols: | ARF1GEF |
NCBI Protein Information: | Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1 |
UniProt Protein Name: | Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1 |
Protein Family: | G-box-binding factor |
UniProt Gene Name: | GBF1 |
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