Anti-citrulline-Histone H3-R2/R8/R17 抗体 (CAB18298)

抗体名:	Anti-citrulline-Histone H3-R2/R8/R17 Antibody
抗体コード:	CAB18298
抗体サイズ:	20uL, 50uL, 100uL
申し込み:	WB IF
反応性:	Human, Mouse, Rat, Other (Wide Range)
宿主種:	Rabbit
免疫原:	A synthetic Peptide of citrulline-Histone H3-R2/R8/R17.

申し込み:	WB IF
推奨希釈:	WB 1:500 - 1:2000 IF 1:50 - 1:200
反応性:	Human, Mouse, Rat, Other (Wide Range)
ポジティブサンプル:	THP-1, Mouse kidney

免疫原:	A synthetic Peptide of citrulline-Histone H3-R2/R8/R17.
精製方法:	Affinity purification
ストレージバッファ:	Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
アイソタイプ:	IgG
順序:	Email for sequence
遺伝子ID:	8350
Uniprot:	P68431
セルラーロケーション:	Chromosome, Nucleus
計算された分子量:	15kDa
観察された分子量:	17KDa

	Western blot analysis of extracts of various cell lines, using citrulline-Histone H3-R2/R8/R17 Rabbit pAb (CAB18298) at 1:500 dilution.THP-1 cells were treated by LPS (1 ug/ml) at 37'C for 8 hours. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Enhanced Kit (RM00021). Exposure time: 180s.
	Immunofluorescence analysis of NIH/3T3 cells using citrulline-Histone H3-R2/R8/R17 antibody (CAB18298) at dilution of 1:100. Blue: DAPI for nuclear staining.

同義語:

H3/A, H3C2, H3C3, H3C4, H3C6, H3C7, H3C8, H3FA, H3C10, H3C11, H3C12, HIST1H3A

バックグラウンド:

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3.

UniProt Protein Function:	H3: Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA. Belongs to the histone H3 family.
UniProt Protein Details:	Protein type:DNA-binding Chromosomal Location of Human Ortholog: 6p22.2 Cellular Component: extracellular region; membrane; nuclear chromosome; nuclear chromosome, telomeric region; nucleoplasm; nucleosome; nucleus; protein complex Molecular Function:cadherin binding; histone binding; protein binding Biological Process: blood coagulation; cellular protein metabolic process; chromatin silencing at rDNA; DNA replication-dependent nucleosome assembly; establishment and/or maintenance of chromatin architecture; negative regulation of gene expression, epigenetic; nucleosome assembly; positive regulation of gene expression, epigenetic; protein heterotetramerization; RNA-mediated gene silencing; telomere organization and biogenesis
NCBI Summary:	Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the small histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq, Aug 2015]
UniProt Code:	P68431
NCBI GenInfo Identifier:	55977055
NCBI Gene ID:	8357
NCBI Accession:	P68431.2
UniProt Secondary Accession:	P68431,P02295, P02296, P16106, Q6ISV8, Q6NWP8, Q6NWP9 Q6NXU4, Q71DJ3, Q93081, A0PJT7, A5PLR1,
UniProt Related Accession:	P68431
Molecular Weight:	15kDa
NCBI Full Name:	Histone H3.1
NCBI Synonym Full Names:	histone cluster 1 H3 family member h
NCBI Official Symbol:	HIST1H3H
NCBI Official Synonym Symbols:	H3/k; H3FK; H3F1K
NCBI Protein Information:	histone H3.1
UniProt Protein Name:	Histone H3.1
UniProt Synonym Protein Names:	Histone H3/a; Histone H3/b; Histone H3/c; Histone H3/d; Histone H3/f; Histone H3/h; Histone H3/i; Histone H3/j; Histone H3/k; Histone H3/l
UniProt Gene Name:	HIST1H3A

Antibodies	ELISA Kits
Anti-MonoMethyl-Histone H3-K27 Antibody (CAB2361)	Histone H3 Colorimetric Cell-Based ELISA Kit
Anti-Asymmetric DiMethyl-Histone H3-R26 Antibody (CAB2375)	Histone H3 (Phospho-Thr3) Colorimetric Cell-Based ELISA Kit
Anti-Asymmetric DiMethyl-Histone H3-R17 Antibody (CAB2421)
Anti-MonoMethyl-Histone H3-R17 Antibody (CAB3151)
Anti-Symmetric DiMethyl-Histone H3-R17 Antibody (CAB3152)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)