Anti-CD74 Antibody (RACO0044)
- SKU:
- RACO0044
- Product type:
- Recombinant Antibody
- Reactivity:
- Human
- Host Species:
- Human
- Isotype:
- IgG
- Application:
- ELISA
- Application:
- IHC
- Application:
- FC
- Conjugation:
- Unconjugated
Frequently bought together:
Description
商品名: | CD74 Antibody |
Product SKU: | RACO0044 |
サイズ: | 50ul |
宿主種: | Human |
申し込み: | ELISA, IHC, FC |
推奨される希釈: | IHC:1:50-1:500 |
反応性: | Human |
免疫原: | A synthesized peptide |
憲法: | Liquid |
ストレージバッファ: | Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
精製方法: | Affinity-chromatography |
抗体のクローン性: | Monoclonal |
アイソタイプ: | Rabbit IgG |
Conjugate: | Non-conjugated |
バックグラウンド: | Plays a critical role in MHC class II antigen processing by stabilizing peptide-free class II alpha/beta heterodimers in a complex soon after their synthesis and directing transport of the complex from the endoplasmic reticulum to the endosomal/lysosomal system where the antigen processing and binding of antigenic peptides to MHC class II takes place. Serves as cell surface receptor for the cytokine MIF. |
シノニム: | HLA class II histocompatibility antigen gamma chain, HLA-DR antigens-associated invariant chain, Ia antigen-associated invariant chain, Ii, p33, CD74, CD74, DHLAG |
IHC image of RACO0044 diluted at 1:100 and staining in paraffin-embedded human endometrial tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. | |
Overlay histogram showing Raji cells stained with RACO0044 (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then permeabilized with 0.3% Triton X-100 for 2 min.The cells were then incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4°C.The secondary antibody used was FITC goat anti-rabbit IgG (H+L) at 1/200 dilution for 1 h at 4°C. Control antibody (green line) was used under the same conditions. Acquisition of >10,000 events was performed. | |
IHC image of RACO0044 diluted at 1:100 and staining in paraffin-embedded human ovarian cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
UniProt Protein Function: | Plays a critical role in MHC class II antigen processing by stabilizing peptide-free class II alpha/beta heterodimers in a complex soon after their synthesis and directing transport of the complex from the endoplasmic reticulum to the endosomal/lysosomal system where the antigen processing and binding of antigenic peptides to MHC class II takes place. Serves as cell surface receptor for the cytokine MIF. |
NCBI Summary: | The protein encoded by this gene associates with class II major histocompatibility complex (MHC) and is an important chaperone that regulates antigen presentation for immune response. It also serves as cell surface receptor for the cytokine macrophage migration inhibitory factor (MIF) which, when bound to the encoded protein, initiates survival pathways and cell proliferation. This protein also interacts with amyloid precursor protein (APP) and suppresses the production of amyloid beta (Abeta). Multiple alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Aug 2011] |
UniProt Code: | P04233 |
NCBI GenInfo Identifier: | 20178292 |
NCBI Gene ID: | 972 |
NCBI Accession: | P04233.3 |
UniProt Secondary Accession: | P04233,Q14597, Q29832, Q5U0J8, Q8SNA0, Q8WLP6, A8K7R1 B4DNE8, D3DQG3, D3DQG4, |
UniProt Related Accession: | P04233 |
Molecular Weight: | |
NCBI Full Name: | HLA class II histocompatibility antigen gamma chain |
NCBI Synonym Full Names: | CD74 molecule |
NCBI Official Symbol: | CD74 |
NCBI Official Synonym Symbols: | II; DHLAG; HLADG; Ia-GAMMA |
NCBI Protein Information: | HLA class II histocompatibility antigen gamma chain |
UniProt Protein Name: | HLA class II histocompatibility antigen gamma chain |
UniProt Synonym Protein Names: | HLA-DR antigens-associated invariant chain; Ia antigen-associated invariant chain; Ii; p33; CD_antigen: CD74 |
Protein Family: | H-2 class II histocompatibility antigen |
UniProt Gene Name: | CD74 |
UniProt Entry Name: | HG2A_HUMAN |