Anti-ATP5A1 Antibody (CAB5884)
- SKU:
- CAB5884
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Metabolism
Description
抗体名: | Anti-ATP5A1 Antibody |
抗体コード: | CAB5884 |
抗体サイズ: | 20uL, 50uL, 100uL |
申し込み: | WB IHC IF IP |
反応性: | Human, Mouse, Rat |
宿主種: | Rabbit |
免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human ATP5A1 (NP_004037.1). |
申し込み: | WB IHC IF IP |
推奨希釈: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:100 IP 1:50 - 1:200 |
反応性: | Human, Mouse, Rat |
ポジティブサンプル: | BT474, SKOV3, HeLa, MCF-7, Mouse kidney, Mouse heart, Rat kidney, Rat heart |
免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human ATP5A1 (NP_004037.1). |
精製方法: | Affinity purification |
ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
アイソタイプ: | IgG |
順序: | MLSV RVAA AVVR ALPR RAGL VSRN ALGS SFIA ARNF HASN THLQ KTGT AEMS SILE ERIL GADT SVDL EETG RVLS IGDG IARV HGLR NVQA EEMV EFSS GLKG MSLN LEPD NVGV VVFG NDKL IKEG DIVK RTGA IVDV PVGE ELLG RVVD ALGN AIDG KGPI GSKT RRRV GLKA PGII PRIS VREP MQTG IKAV DSLV PIGR GQRE LIIG DRQT GKTS IAID TIIN QKRF NDGS DEKK KLYC IYVA IGQK RSTV AQLV KRLT DADA MKYT IVVS ATAS |
遺伝子ID: | 498 |
Uniprot: | P25705 |
セルラーロケーション: | Cell membrane, Extracellular side, Mitochondrion inner membrane, Peripheral membrane protein |
計算された分子量: | 54kDa/57kDa/59kDa |
観察された分子量: | 53kDa |
同義語: | ATP5A1, ATP5A, ATP5AL2, ATPM, COXPD22, HEL-S-123m, MC5DN4, MOM2, OMR, ORM, hATP1 |
バックグラウンド: | This gene encodes a subunit of mitochondrial ATP synthase. Mitochondrial ATP synthase catalyzes ATP synthesis, using an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. ATP synthase is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, comprising the proton channel. The catalytic portion of mitochondrial ATP synthase consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled with a stoichiometry of 3 alpha, 3 beta, and a single representative of the other 3. The proton channel consists of three main subunits (a, b, c). This gene encodes the alpha subunit of the catalytic core. Alternatively spliced transcript variants encoding the different isoforms have been identified. Pseudogenes of this gene are located on chromosomes 9, 2, and 16. |
UniProt Protein Function: | ATP5A1: Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core, and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Subunits alpha and beta form the catalytic core in F(1). Rotation of the central stalk against the surrounding alpha(3)beta(3) subunits leads to hydrolysis of ATP in three separate catalytic sites on the beta subunits. Subunit alpha does not bear the catalytic high-affinity ATP-binding sites. Belongs to the ATPase alpha/beta chains family. |
UniProt Protein Details: | Protein type:EC 3.6.3.14; Transporter; Mitochondrial; Hydrolase; Membrane protein, integral; Energy Metabolism - oxidative phosphorylation Chromosomal Location of Human Ortholog: 18q21 Cellular Component: membrane; mitochondrial inner membrane; mitochondrial matrix; mitochondrial proton-transporting ATP synthase complex; mitochondrion; plasma membrane; signalosome Molecular Function:ATP binding; ATPase activity; hydrogen ion transporting ATP synthase activity, rotational mechanism; MHC class I protein binding; protein binding; transmembrane transporter activity Biological Process: ATP biosynthetic process; embryonic development; lipid metabolic process; mitochondrial ATP synthesis coupled proton transport; negative regulation of endothelial cell proliferation Disease: Combined Oxidative Phosphorylation Deficiency 22; Mitochondrial Complex V (atp Synthase) Deficiency, Nuclear Type 4 |
NCBI Summary: | This gene encodes a subunit of mitochondrial ATP synthase. Mitochondrial ATP synthase catalyzes ATP synthesis, using an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. ATP synthase is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, comprising the proton channel. The catalytic portion of mitochondrial ATP synthase consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled with a stoichiometry of 3 alpha, 3 beta, and a single representative of the other 3. The proton channel consists of three main subunits (a, b, c). This gene encodes the alpha subunit of the catalytic core. Alternatively spliced transcript variants encoding the different isoforms have been identified. Pseudogenes of this gene are located on chromosomes 9, 2, and 16. [provided by RefSeq, Mar 2012] |
UniProt Code: | P25705 |
NCBI GenInfo Identifier: | 114517 |
NCBI Gene ID: | 498 |
NCBI Accession: | P25705.1 |
UniProt Secondary Accession: | P25705,Q53XX6, Q8IXV2, Q96FB4, Q96HW2, Q96IR6, Q9BTV8 A8K092, B4DY56, K7ENP3, |
UniProt Related Accession: | P25705 |
Molecular Weight: | 57,547 Da |
NCBI Full Name: | ATP synthase subunit alpha, mitochondrial |
NCBI Synonym Full Names: | ATP synthase, H+ transporting, mitochondrial F1 complex, alpha subunit 1, cardiac muscle |
NCBI Official Symbol: | ATP5A1 |
NCBI Official Synonym Symbols: | OMR; ORM; ATPM; MOM2; ATP5A; hATP1; MC5DN4; ATP5AL2; COXPD22; HEL-S-123m |
NCBI Protein Information: | ATP synthase subunit alpha, mitochondrial |
UniProt Protein Name: | ATP synthase subunit alpha, mitochondrial |
UniProt Gene Name: | ATP5A1 |
UniProt Entry Name: | ATPA_HUMAN |