Anti-AKT1S1 Antibody (CAB6238)
- SKU:
- CAB6238
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Frequently bought together:
Description
抗体名: | Anti-AKT1S1 Antibody |
抗体コード: | CAB6238 |
抗体サイズ: | 20uL, 50uL, 100uL |
申し込み: | WB IHC IF |
反応性: | Human, Mouse, Rat |
宿主種: | Rabbit |
免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 152-276 of human AKT1S1 (NP_115751.3). |
申し込み: | WB IHC IF |
推奨希釈: | WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:50 - 1:100 |
反応性: | Human, Mouse, Rat |
ポジティブサンプル: | Mouse heart, Rat heart |
免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 152-276 of human AKT1S1 (NP_115751.3). |
精製方法: | Affinity purification |
ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
アイソタイプ: | IgG |
順序: | DEDA TLQD LPPF CESD PEST DDGS LSEE TPAG PPTC SVPP ASAL PTQQ YAKS LPVS VPVW GFKE KRTE ARSS DEEN GPPS SPDL DRIA ASMR ALVL REAE DTQV FGDL PRPR LNTS DFQK LKRK Y |
遺伝子ID: | 84335 |
Uniprot: | Q96B36 |
セルラーロケーション: | Cytoplasm, cytosol |
計算された分子量: | 13kDa/27kDa/29kDa |
観察された分子量: | 50kDa |
同義語: | AKT1S1, Lobe, PRAS40 |
バックグラウンド: |
UniProt Protein Function: | Subunit of mTORC1, which regulates cell growth and survival in response to nutrient and hormonal signals. mTORC1 is activated in response to growth factors or amino acids. Growth factor-stimulated mTORC1 activation involves a AKT1-mediated phosphorylation of TSC1-TSC2, which leads to the activation of the RHEB GTPase that potently activates the protein kinase activity of mTORC1. Amino acid-signaling to mTORC1 requires its relocalization to the lysosomes mediated by the Ragulator complex and the Rag GTPases. Activated mTORC1 up-regulates protein synthesis by phosphorylating key regulators of mRNA translation and ribosome synthesis. mTORC1 phosphorylates EIF4EBP1 and releases it from inhibiting the elongation initiation factor 4E (eiF4E). mTORC1 phosphorylates and activates S6K1 at 'Thr-389', which then promotes protein synthesis by phosphorylating PDCD4 and targeting it for degradation. Within mTORC1, AKT1S1 negatively regulates mTOR activity in a manner that is dependent on its phosphorylation state and binding to 14-3-3 proteins. Inhibits RHEB-GTP-dependent mTORC1 activation. Substrate for AKT1 phosphorylation, but can also be activated by AKT1-independent mechanisms. May also play a role in nerve growth factor-mediated neuroprotection. |
NCBI Summary: | AKT1S1 is a proline-rich substrate of AKT (MIM 164730) that binds 14-3-3 protein (see YWHAH, MIM 113508) when phosphorylated (Kovacina et al., 2003 [PubMed 12524439]).[supplied by OMIM, Mar 2008] |
UniProt Code: | Q96B36 |
NCBI GenInfo Identifier: | 74731194 |
NCBI Gene ID: | 84335 |
NCBI Accession: | Q96B36.1 |
UniProt Secondary Accession: | Q96B36,Q96BI4, Q96IK7, Q96NG2, Q9BWR5, A8MTQ1, B2RE93 J3KPM3, |
UniProt Related Accession: | Q96B36 |
Molecular Weight: | 29,262 Da |
NCBI Full Name: | Proline-rich AKT1 substrate 1 |
NCBI Synonym Full Names: | AKT1 substrate 1 |
NCBI Official Symbol: | AKT1S1 |
NCBI Official Synonym Symbols: | Lobe; PRAS40 |
NCBI Protein Information: | proline-rich AKT1 substrate 1 |
UniProt Protein Name: | Proline-rich AKT1 substrate 1 |
UniProt Synonym Protein Names: | 40 kDa proline-rich AKT substrate |
Protein Family: | Proline-rich AKT1 substrate |
UniProt Gene Name: | AKT1S1 |
UniProt Entry Name: | AKTS1_HUMAN |
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