ACOT8 Antibody (PACO61899)
- SKU:
- PACO61899
- Product type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Application:
- ELISA
- Application:
- IHC
- Antibody type:
- Polyclonal
- Conjugation:
- Unconjugated
Frequently bought together:
Description
抗体名: | ACOT8 Antibody (PACO61899) |
抗体コード: | PACO61899 |
サイズ: | 50ul |
宿主種: | Rabbit |
申し込み: | ELISA, IHC |
推奨される希釈: | ELISA:1:2000-1:10000, IHC:1:20-1:200 |
反応性: | Human |
免疫原: | Recombinant Human Acyl-coenzyme A thioesterase 8 protein (1-200AA) |
憲法: | Liquid |
ストレージバッファ: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
精製方法: | Antigen Affinity Purified |
抗体のクローン性: | Polyclonal |
アイソタイプ: | IgG |
Conjugate: | Non-conjugated |
IHC image of PACO61899 diluted at 1:100 and staining in paraffin-embedded human liver tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
バックグラウンド: | Acyl-coenzyme A (acyl-CoA) thioesterases are a group of enzymes that catalyze the hydrolysis of acyl-CoAs to the free fatty acid, and coenzyme A (CoASH), providing the potential to regulate intracellular levels of acyl-CoAs, free fatty acid, and CoASH. Competes with bile acid, CoA: amino acid, N-acyltransferase (BAAT) for bile acid, CoA substrate (such as chenodeoxycholoyl-CoA). Shows a preference for medium-length fatty acyl-CoAs (C2 to C20). Inactive towards substrates with more than C20 aliphatic chains. Involved in the metabolic regulation of peroxisome proliferation. |
シノニム: | Acyl-coenzyme A thioesterase 8 (Acyl-CoA thioesterase 8) (EC 3.1.2.27) (Choloyl-coenzyme A thioesterase) (HIV-Nef-associated acyl-CoA thioesterase) (PTE-2) (Peroxisomal acyl-coenzyme A thioester hydrolase 1) (PTE-1) (Peroxisomal long-chain acyl-CoA thioesterase 1) (Thioesterase II) (hACTE-III) (hACTEIII) (hTE), ACOT8, ACTEIII PTE1 PTE2 |
UniProt Protein Function: | ACOT8: Acyl-CoA thioesterases are a group of enzymes that catalyze the hydrolysis of acyl-CoAs to the free fatty acid and coenzyme A (CoASH), providing the potential to regulate intracellular levels of acyl-CoAs, free fatty acids and CoASH. May mediate Nef-induced down-regulation of CD4. Major thioesterase in peroxisomes. Competes with BAAT (Bile acid CoA: amino acid N- acyltransferase) for bile acid-CoA substrate (such as chenodeoxycholoyl-CoA). Shows a preference for medium-length fatty acyl-CoAs. May be involved in the metabolic regulation of peroxisome proliferation. Belongs to the C/M/P thioester hydrolase family. |
UniProt Protein Details: | Protein type:EC 3.1.2.27; Hydrolase Chromosomal Location of Human Ortholog: 20q13.12 Cellular Component: peroxisomal matrix Molecular Function:acetyl-CoA hydrolase activity; acyl-CoA hydrolase activity; CoA hydrolase activity; palmitoyl-CoA hydrolase activity; protein binding; receptor binding Biological Process: acyl-CoA metabolic process; bile acid biosynthetic process; dicarboxylic acid catabolic process; fatty acid beta-oxidation using acyl-CoA oxidase; negative regulation of CD4 biosynthetic process; peroxisome fission |
NCBI Summary: | The protein encoded by this gene is a peroxisomal thioesterase that appears to be involved more in the oxidation of fatty acids rather than in their formation. The encoded protein can bind to the human immunodeficiency virus-1 protein Nef, and mediate Nef-induced down-regulation of CD4 in T-cells. [provided by RefSeq, Oct 2010] |
UniProt Code: | O14734 |
NCBI GenInfo Identifier: | 14285685 |
NCBI Gene ID: | 10005 |
NCBI Accession: | O14734.1 |
UniProt Secondary Accession: | O14734,O15261, Q17RX4, |
UniProt Related Accession: | O14734 |
Molecular Weight: | 35,914 Da |
NCBI Full Name: | Acyl-coenzyme A thioesterase 8 |
NCBI Synonym Full Names: | acyl-CoA thioesterase 8 |
NCBI Official Symbol: | ACOT8 |
NCBI Official Synonym Symbols: | hTE; NAP1; PTE1; PTE2; PTE-1; PTE-2; HNAACTE; hACTE-III |
NCBI Protein Information: | acyl-coenzyme A thioesterase 8 |
UniProt Protein Name: | Acyl-coenzyme A thioesterase 8 |
UniProt Synonym Protein Names: | Choloyl-coenzyme A thioesterase; HIV-Nef-associated acyl-CoA thioesterase; PTE-2; Peroxisomal acyl-coenzyme A thioester hydrolase 1; PTE-1; Peroxisomal long-chain acyl-CoA thioesterase 1; Thioesterase II; hACTE-III; hACTEIII; hTE |
Protein Family: | Acyl-coenzyme A thioesterase |
UniProt Gene Name: | ACOT8 |
UniProt Entry Name: | ACOT8_HUMAN |