WIPI1 Antibody (PACO43809)
- SKU:
- PACO43809
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Application:
- ELISA
- Application:
- WB
- Application:
- IHC
- Antibody type:
- Polyclonal
- Conjugation:
- Unconjugated
Frequently bought together:
Description
抗体名: | WIPI1 Antibody (PACO43809) |
抗体コード: | PACO43809 |
サイズ: | 50ul |
宿主種: | Rabbit |
申し込み: | ELISA, WB, IHC |
推奨される希釈: | ELISA:1:2000-1:10000, WB:1:500-1:2000, IHC:1:20-1:200 |
反応性: | Human, Mouse |
免疫原: | Recombinant Human WD repeat domain phosphoinositide-interacting protein 1 protein (187-446AA) |
憲法: | Liquid |
ストレージバッファ: | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
精製方法: | Antigen Affinity Purified |
抗体のクローン性: | Polyclonal |
アイソタイプ: | IgG |
Conjugate: | Non-conjugated |
Western blot. All lanes: WIPI1 antibody at 6µg/ml. Lane 1: 293T whole cell lysate. Lane 2: NIH/3T3 whole cell lysate. Lane 3: Mouse skeletal muscle tissue. Secondary. Goat polyclonal to rabbit IgG at 1/10000 dilution. Predicted band size: 49 kDa. Observed band size: 49 kDa. | |
Immunohistochemistry of paraffin-embedded human placenta tissue using PACO43809 at dilution of 1:100. |
バックグラウンド: | Plays an important role in autophagy and in particular starvation- and calcium-mediated autophagy, as well as in mitophagy. Functions upstream of the ATG12-ATG5-ATG16L1 complex and LC3, and downstream of the ULK1 and PI3-kinase complexes. Involved in xenophagy of Staphylococcus aureus. Invading S.aureus cells become entrapped in autophagosome-like WIPI1 positive vesicles targeted for lysosomal degradation. Plays also a distinct role in controlling the transcription of melanogenic enzymes and melanosome maturation, a process that is distinct from starvation-induced autophagy. May also regulate the trafficking of proteins involved in the mannose-6-phosphate receptor (MPR) recycling pathway. |
シノニム: | WD repeat domain phosphoinositide-interacting protein 1 (WIPI-1) (Atg18 protein homolog) (WD40 repeat protein interacting with phosphoinositides of 49 kDa) (WIPI 49 kDa), WIPI1, WIPI49 |
UniProt Protein Function: | ATG18: May play a role in autophagy. May regulate the trafficking of proteins involved in the mannose-6-phosphate receptor (MPR) recycling pathway. Probably interacts with androgen (AR) and the estrogen receptor (ER). Binds PtdIns3P and to a lesser extent, PtdIns3,5P2 and PtdIns5P in vitro. Interaction with PtdIns3P is required for recruitment to membranes. 2 isoforms of the human protein are produced by alternative splicing. |
UniProt Protein Details: | Protein type:Autophagy Chromosomal Location of Human Ortholog: 17q24.2 Cellular Component: cytoplasm; cytosol; endosome membrane; extrinsic to membrane; Golgi membrane; pre-autophagosomal structure membrane; trans-Golgi network Molecular Function:androgen receptor binding; estrogen receptor binding; phosphatidylinositol 3-phosphate binding; receptor binding Biological Process: autophagic vacuole formation; autophagy; macroautophagy; mitochondrion degradation; protein amino acid lipidation; vesicle targeting, trans-Golgi to endosome |
NCBI Summary: | This gene encodes a WD40 repeat protein. Members of the WD40 repeat family are key components of many essential biologic functions. They regulate the assembly of multiprotein complexes by presenting a beta-propeller platform for simultaneous and reversible protein-protein interactions. Members of the WIPI subfamily of WD40 repeat proteins have a 7-bladed propeller structure and contain a conserved motif for interaction with phospholipids. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2016] |
UniProt Code: | Q5MNZ9 |
NCBI GenInfo Identifier: | 146291100 |
NCBI Gene ID: | 55062 |
NCBI Accession: | Q5MNZ9.3 |
UniProt Secondary Accession: | Q5MNZ9,Q8IXM5, Q9NWF8, |
UniProt Related Accession: | Q5MNZ9 |
Molecular Weight: | 48,202 Da |
NCBI Full Name: | WD repeat domain phosphoinositide-interacting protein 1 |
NCBI Synonym Full Names: | WD repeat domain, phosphoinositide interacting 1 |
NCBI Official Symbol: | WIPI1 |
NCBI Official Synonym Symbols: | ATG18; ATG18A; WIPI49 |
NCBI Protein Information: | WD repeat domain phosphoinositide-interacting protein 1 |
UniProt Protein Name: | WD repeat domain phosphoinositide-interacting protein 1 |
UniProt Synonym Protein Names: | Atg18 protein homolog; WD40 repeat protein interacting with phosphoinositides of 49 kDa; WIPI 49 kDa |
Protein Family: | WD repeat domain phosphoinositide-interacting protein |
UniProt Gene Name: | WIPI1 |
UniProt Entry Name: | WIPI1_HUMAN |