TNP2 Antibody (PACO60853)
- SKU:
- PACO60853
- Product type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Application:
- ELISA
- Application:
- IHC
- Application:
- IF
- Antibody type:
- Polyclonal
- Conjugation:
- Unconjugated
Frequently bought together:
Description
抗体名: | TNP2 Antibody (PACO60853) |
抗体コード: | PACO60853 |
サイズ: | 50ug |
宿主種: | Rabbit |
申し込み: | ELISA, IHC, IF |
推奨される希釈: | ELISA:1:2000-1:10000, IHC:1:200-1:500, IF:1:50-1:200 |
反応性: | Human |
免疫原: | Recombinant Human Nuclear transition protein 2 protein (1-138AA) |
憲法: | Liquid |
ストレージバッファ: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
精製方法: | >95%, Protein G purified |
抗体のクローン性: | Polyclonal |
アイソタイプ: | IgG |
Conjugate: | Non-conjugated |
IHC image of PACO60853 diluted at 1:200 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. | |
Immunofluorescence staining of Hela cells with PACO60853 at 1:66, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). |
バックグラウンド: | Plays a key role in the replacement of histones to protamine in the elongating spermatids of mammals. In condensing spermatids, loaded onto the nucleosomes, where it promotes the recruitment and processing of protamines, which are responsible for histone eviction. |
シノニム: | Nuclear transition protein 2 (TP-2) (TP2), TNP2 |
UniProt Protein Function: | TP2: In the elongating spermatids of mammals, the conversion of nucleosomal chromatin to the compact, non-nucleosomal form found in the sperm nucleus is associated with the appearance of a small set of basic chromosomal transition proteins. Belongs to the nuclear transition protein 2 family. |
UniProt Protein Details: | Protein type:DNA-binding Chromosomal Location of Human Ortholog: 16p13.13 Cellular Component: nucleosome; nucleus Molecular Function:DNA binding; metal ion binding Biological Process: sperm motility; binding of sperm to zona pellucida; multicellular organismal development; acrosome reaction; penetration of zona pellucida; spermatogenesis; cell differentiation |
UniProt Code: | Q05952 |
NCBI GenInfo Identifier: | 464818 |
NCBI Gene ID: | 7142 |
NCBI Accession: | Q05952.1 |
UniProt Secondary Accession: | Q05952,Q9NZB0, |
UniProt Related Accession: | Q05952 |
Molecular Weight: | 138 |
NCBI Full Name: | Nuclear transition protein 2 |
NCBI Synonym Full Names: | transition protein 2 (during histone to protamine replacement) |
NCBI Official Symbol: | TNP2 |
NCBI Official Synonym Symbols: | TP2 |
NCBI Protein Information: | nuclear transition protein 2; TP-2 |
UniProt Protein Name: | Nuclear transition protein 2 |
Protein Family: | Nuclear transition protein |
UniProt Gene Name: | TNP2 |
UniProt Entry Name: | STP2_HUMAN |
Antibodies |
Anti-TNP2 Antibody (CAB6773) |
Secondary Antibody |
Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014) |
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