TCF12 Antibody (PACO44165)
- SKU:
- PACO44165
- Product type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Application:
- ELISA
- Application:
- IHC
- Application:
- IP
- Antibody type:
- Polyclonal
- Conjugation:
- Unconjugated
Frequently bought together:
Description
抗体名: | TCF12 Antibody (PACO44165) |
抗体コード: | PACO44165 |
サイズ: | 50ul |
宿主種: | Rabbit |
申し込み: | ELISA, IHC, IP |
推奨される希釈: | ELISA:1:2000-1:10000, IHC:1:20-1:200, IP:1:200-1:2000 |
反応性: | Human |
免疫原: | Recombinant Human Transcription factor 12 protein (443-682AA) |
憲法: | Liquid |
ストレージバッファ: | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
精製方法: | Antigen Affinity Purified |
抗体のクローン性: | Polyclonal |
アイソタイプ: | IgG |
Conjugate: | Non-conjugated |
Immunohistochemistry of paraffin-embedded human thymus tissue using PACO44165 at dilution of 1:100. | |
Immunoprecipitating TCF12 in Jurkat whole cell lysate. Lane 1: Rabbit control IgG instead of (1µg) instead of PACO44165 in Jurkat whole cell lysate.For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the Secondary antibody (1/50000). Lane 2: PACO44165 (4µg) + Jurkat whole cell lysate (500µg). Lane 3: Jurkat whole cell lysate (20µg). |
バックグラウンド: | Transcriptional regulator. Involved in the initiation of neuronal differentiation. Activates transcription by binding to the E box (5'-CANNTG-3'). |
シノニム: | Transcription factor 12 (TCF-12) (Class B basic helix-loop-helix protein 20) (bHLHb20) (DNA-binding protein HTF4) (E-box-binding protein) (Transcription factor HTF-4), TCF12, BHLHB20 HEB HTF4 |
UniProt Protein Function: | TCF12 iso3: Binds specifically to oligomers of E-box motifs. May play important roles during development of the nervous system as well as in other organ systems. Efficient DNA binding requires dimerization with another bHLH protein. Forms homo- or hetero-oligomers with myogenin, E12 and ITF2 proteins. Interacts with PTF1A. |
UniProt Protein Details: | Protein type:DNA-binding; Transcription factor Chromosomal Location of Human Ortholog: 15q21 Cellular Component: nuclear chromatin; cytoplasm; nucleus Molecular Function:protein binding; protein heterodimerization activity; bHLH transcription factor binding; SMAD binding; transcription factor binding; transcription factor activity Biological Process: regulation of transcription from RNA polymerase II promoter; muscle development; transcription, DNA-dependent; positive regulation of transcription from RNA polymerase II promoter; immune response; positive regulation of neuron differentiation |
NCBI Summary: | The protein encoded by this gene is a member of the basic helix-loop-helix (bHLH) E-protein family that recognizes the consensus binding site (E-box) CANNTG. This encoded protein is expressed in many tissues, among them skeletal muscle, thymus, B- and T-cells, and may participate in regulating lineage-specific gene expression through the formation of heterodimers with other bHLH E-proteins. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined. [provided by RefSeq, Jul 2008] |
UniProt Code: | Q99081 |
NCBI GenInfo Identifier: | 1708332 |
NCBI Gene ID: | 6938 |
NCBI Accession: | Q99081.1 |
UniProt Related Accession: | Q99081 |
Molecular Weight: | |
NCBI Full Name: | Transcription factor 12 |
NCBI Synonym Full Names: | transcription factor 12 |
NCBI Official Symbol: | TCF12 |
NCBI Official Synonym Symbols: | HEB; p64; CRS3; HTF4; TCF-12; bHLHb20; HsT17266 |
NCBI Protein Information: | transcription factor 12 |
UniProt Protein Name: | Transcription factor 12 |
UniProt Synonym Protein Names: | Class B basic helix-loop-helix protein 20; bHLHb20; DNA-binding protein HTF4; E-box-binding protein; Transcription factor HTF-4 |
Protein Family: | Transcription factor |
UniProt Gene Name: | TCF12 |
UniProt Entry Name: | HTF4_HUMAN |