TAF15 Antibody (PACO56094)
- SKU:
- PACO56094
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Rat
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Application:
- ELISA
- Application:
- WB
- Application:
- IHC
- Application:
- IF
- Antibody type:
- Polyclonal
- Conjugation:
- Unconjugated
Frequently bought together:
Description
抗体名: | TAF15 Antibody (PACO56094) |
抗体コード: | PACO56094 |
サイズ: | 50ug |
宿主種: | Rabbit |
申し込み: | ELISA, WB, IHC, IF |
推奨される希釈: | ELISA:1:2000-1:10000, WB:1:500-1:5000, IHC:1:100-1:300, IF:1:50-1:200 |
反応性: | Human, Rat, Mouse |
免疫原: | Recombinant Human TATA-binding protein-associated factor 2N protein (93-206AA) |
憲法: | Liquid |
ストレージバッファ: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
精製方法: | >95%, Protein G purified |
抗体のクローン性: | Polyclonal |
アイソタイプ: | IgG |
Conjugate: | Non-conjugated |
Western Blot. Positive WB detected in: Jurkat whole cell lysate, A549 whole cell lysate, 293 whole cell lysate, Hela whole cell lysate. All lanes: TAF15 antibody at 8.5µg/ml. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 62 kDa. Observed band size: 62 kDa. | |
Immunofluorescence staining of Hela cells with PACO56094 at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). | |
IHC image of PACO56094 diluted at 1:300 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
バックグラウンド: | RNA and ssDNA-binding protein that may play specific roles during transcription initiation at distinct promoters. Can enter the preinitiation complex together with the RNA polymerase II (Pol II). |
シノニム: | TATA-binding protein-associated factor 2N (68 kDa TATA-binding protein-associated factor) (TAF(II)68) (TAFII68) (RNA-binding protein 56), TAF15, RBP56 TAF2N |
UniProt Protein Function: | TAF15: RNA and ssDNA-binding protein that may play specific roles during transcription initiation at distinct promoters. Can enter the preinitiation complex together with the RNA polymerase II (Pol II). A chromosomal aberration involving TAF15/TAF2N is found in a form of extraskeletal myxoid chondrosarcomas (EMC). Translocation t(9;17)(q22;q11) with NR4A3. Belongs to the RRM TET family. 2 isoforms of the human protein are produced by alternative splicing. |
UniProt Protein Details: | Protein type:RNA-binding; Oncoprotein; Translation Chromosomal Location of Human Ortholog: 17q11.1-q11.2 Cellular Component: cytoplasm; nucleolus; nucleoplasm; nucleus Molecular Function:DNA binding; nucleotide binding; protein binding; zinc ion binding Biological Process: positive regulation of transcription, DNA-dependent Disease: Chondrosarcoma, Extraskeletal Myxoid |
NCBI Summary: | This gene encodes a member of the TET family of RNA-binding proteins. The encoded protein plays a role in RNA polymerase II gene transcription as a component of a distinct subset of multi-subunit transcription initiation factor TFIID complexes. Translocations involving this gene play a role in acute leukemia and extraskeletal myxoid chondrosarcoma, and mutations in this gene may play a role in amyotrophic lateral sclerosis. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, May 2012] |
UniProt Code: | Q92804 |
NCBI GenInfo Identifier: | 8928305 |
NCBI Gene ID: | 8148 |
NCBI Accession: | Q92804.1 |
UniProt Secondary Accession: | Q92804,Q86X94, Q92751, B2R837, |
UniProt Related Accession: | Q92804 |
Molecular Weight: | 61,558 Da |
NCBI Full Name: | TATA-binding protein-associated factor 2N |
NCBI Synonym Full Names: | TATA-box binding protein associated factor 15 |
NCBI Official Symbol: | TAF15 |
NCBI Official Synonym Symbols: | Npl3; RBP56; TAF2N; TAFII68 |
NCBI Protein Information: | TATA-binding protein-associated factor 2N |
UniProt Protein Name: | TATA-binding protein-associated factor 2N |
UniProt Synonym Protein Names: | 68 kDa TATA-binding protein-associated factor; TAF(II)68; TAFII68; RNA-binding protein 56 |
Protein Family: | Transcription initiation factor |
UniProt Gene Name: | TAF15 |
UniProt Entry Name: | RBP56_HUMAN |