SEPT11 Antibody (PACO63999)
- SKU:
- PACO63999
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Application:
- ELISA
- Application:
- WB
- Application:
- IHC
- Application:
- IF
- Antibody type:
- Polyclonal
- Conjugation:
- Unconjugated
Frequently bought together:
Description
抗体名: | SEPT11 Antibody (PACO63999) |
抗体コード: | PACO63999 |
サイズ: | 50ul |
宿主種: | Rabbit |
申し込み: | ELISA, WB, IHC, IF |
推奨される希釈: | ELISA:1:2000-1:10000, WB:1:1000-1:5000, IHC:1:200-1:500, IF:1:50-1:200 |
反応性: | Human, Rat |
免疫原: | Recombinant Human Septin-11 protein (237-429AA) |
憲法: | Liquid |
ストレージバッファ: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
精製方法: | >95%, Antigen Affinity purified & Affinity purified |
抗体のクローン性: | Polyclonal |
アイソタイプ: | IgG |
Conjugate: | Non-conjugated |
Western Blot. Positive WB detected in: SH-SY5Y whole cell lysate, Rat brain tissue, Rat liver tissue. All lanes: SEPT11 antibody at 1:2000. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 50, 51 kDa. Observed band size: 50 kDa. | |
Immunofluorescence staining of Hela cells with PACO63999 at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). | |
IHC image of PACO63999 diluted at 1:400 and staining in paraffin-embedded human small intestine tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
バックグラウンド: | Filament-forming cytoskeletal GTPase. May play a role in cytokinesis (Potential). May play a role in the cytoarchitecture of neurons, including dendritic arborization and dendritic spines, and in GABAergic synaptic connectivity (By similarity). During Listeria monocytogenes infection, not required for the bacterial entry process, but restricts its efficacy. |
シノニム: | Septin-11, SEPT11 |
UniProt Protein Function: | SEPT11: Filament-forming cytoskeletal GTPase. May play a role in cytokinesis (Potential). May play a role in the cytoarchitecture of neurons, including dendritic arborization and dendritic spines, and in GABAergic synaptic connectivity. During Listeria monocytogenes infection, not required for the bacterial entry process, but restricts its efficacy. A chromosomal aberration involving SEPT11 may be a cause of chronic neutrophilic leukemia. Translocation t(4;11)(q21;q23) with MLL/HRX. Belongs to the septin family. 2 isoforms of the human protein are produced by alternative splicing. |
UniProt Protein Details: | Protein type:Cell cycle regulation; Cytoskeletal; Hydrolase; Motility/polarity/chemotaxis Chromosomal Location of Human Ortholog: 4q21.1 Cellular Component: stress fiber Molecular Function:protein binding Biological Process: protein heterooligomerization |
NCBI Summary: | SEPT11 belongs to the conserved septin family of filament-forming cytoskeletal GTPases that are involved in a variety of cellular functions including cytokinesis and vesicle trafficking (Hanai et al., 2004 [PubMed 15196925]; Nagata et al., 2004 [PubMed 15485874]).[supplied by OMIM, Jul 2009] |
UniProt Code: | Q9NVA2 |
NCBI GenInfo Identifier: | 50401687 |
NCBI Gene ID: | 55752 |
NCBI Accession: | Q9NVA2.3 |
UniProt Secondary Accession: | Q9NVA2,Q4W5G1, Q7L4N1, Q96SP1, Q9UFY9, B7Z7Z6, E9KL32 |
UniProt Related Accession: | Q9NVA2 |
Molecular Weight: | 49kDa |
NCBI Full Name: | Septin-11 |
NCBI Synonym Full Names: | septin 11 |
NCBI Official Symbol: | SEPT11 |
NCBI Protein Information: | septin-11 |
UniProt Protein Name: | Septin-11 |
UniProt Gene Name: | SEPT11 |