RNF14 Antibody (PACO17002)
- SKU:
- PACO17002
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Application:
- ELISA
- Application:
- WB
- Application:
- IHC
- Antibody type:
- Polyclonal
- Conjugation:
- Unconjugated
Description
抗体名: | RNF14 Antibody (PACO17002) |
抗体コード: | PACO17002 |
サイズ: | 50ul |
宿主種: | Rabbit |
申し込み: | ELISA, WB, IHC |
推奨される希釈: | ELISA:1:1000-1:2000, WB:1:200-1:1000, IHC:1:25-1:100 |
反応性: | Human, Mouse |
免疫原: | Fusion protein of human RNF14 |
憲法: | Liquid |
ストレージバッファ: | -20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol |
精製方法: | Antigen affinity purification |
抗体のクローン性: | Polyclonal |
アイソタイプ: | IgG |
Conjugate: | Non-conjugated |
Gel: 8%SDS-PAGE, Lysate: 40 μg, Lane: RAW264.7 cells, Primary antibody: PACO17002(RNF14 Antibody) at dilution 1/300, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 2 minutes. | |
The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using PACO17002(RNF14 Antibody) at dilution 1/20, on the right is treated with fusion protein. (Original magnification: x200). |
バックグラウンド: | The protein encoded by this gene contains a RING zinc finger, a motif known to be involved in protein-protein interactions. This protein interacts with androgen receptor (AR) and may function as a coactivator that induces AR target gene expression in prostate. A dominant negative mutant of this gene has been demonstrated to inhibit the AR-mediated growth of prostate cancer. This protein also interacts with class III ubiquitin-conjugating enzymes (E2s) and may act as a ubiquitin-ligase (E3) in the ubiquitination of certain nuclear proteins. |
シノニム: | ring finger protein 14 |
UniProt Protein Function: | RNF14: Might act as an E3 ubiquitin-protein ligase which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes and then transfers it to substrates, which could be nuclear proteins. Could play a role as a coactivator for androgen- and, to a lesser extent, progesterone-dependent transcription. Belongs to the RBR family. RNF14 subfamily. |
UniProt Protein Details: | Protein type:Nuclear receptor co-regulator; EC 6.3.2.-; Ubiquitin conjugating system; Ligase; EC 6.3.2.19; Transcription, coactivator/corepressor; Ubiquitin ligase Chromosomal Location of Human Ortholog: 5q23.3-q31.1 Cellular Component: cytoplasm; nucleus; ubiquitin ligase complex Molecular Function:androgen receptor binding; protein binding; small conjugating protein ligase activity; transcription coactivator activity; ubiquitin conjugating enzyme binding Biological Process: positive regulation of proteasomal ubiquitin-dependent protein catabolic process; positive regulation of transcription, DNA-dependent; protein polyubiquitination; protein ubiquitination; protein ubiquitination during ubiquitin-dependent protein catabolic process; regulation of transcription from RNA polymerase II promoter; regulation of transcription, DNA-dependent; signal transduction |
NCBI Summary: | The protein encoded by this gene contains a RING zinc finger, a motif known to be involved in protein-protein interactions. This protein interacts with androgen receptor (AR) and may function as a coactivator that induces AR target gene expression in prostate. A dominant negative mutant of this gene has been demonstrated to inhibit the AR-mediated growth of prostate cancer. This protein also interacts with class III ubiquitin-conjugating enzymes (E2s) and may act as a ubiquitin-ligase (E3) in the ubiquitination of certain nuclear proteins. Six alternatively spliced transcript variants encoding two distinct isoforms have been reported. [provided by RefSeq, Jan 2011] |
UniProt Code: | Q9UBS8 |
NCBI GenInfo Identifier: | 17380293 |
NCBI Gene ID: | 9604 |
NCBI Accession: | Q9UBS8.1 |
UniProt Secondary Accession: | Q9UBS8,O94793, Q6IBV0, A0AV26, A6NMR2, A8MTW5, B3KN72 B7ZLV2, D3DQE4, |
UniProt Related Accession: | Q9UBS8 |
Molecular Weight: | 39,634 Da |
NCBI Full Name: | E3 ubiquitin-protein ligase RNF14 |
NCBI Synonym Full Names: | ring finger protein 14 |
NCBI Official Symbol: | RNF14 |
NCBI Official Synonym Symbols: | ARA54; HFB30; TRIAD2; HRIHFB2038 |
NCBI Protein Information: | E3 ubiquitin-protein ligase RNF14 |
UniProt Protein Name: | E3 ubiquitin-protein ligase RNF14 |
UniProt Synonym Protein Names: | Androgen receptor-associated protein 54; HFB30; RING finger protein 14; Triad2 protein |
Protein Family: | E3 ubiquitin-protein ligase |
UniProt Gene Name: | RNF14 |
UniProt Entry Name: | RNF14_HUMAN |