Ripk1 Antibody (PACO52974)
- SKU:
- PACO52974
- Product type:
- Antibody
- Reactivity:
- Mouse
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Application:
- ELISA
- Application:
- IP
- Antibody type:
- Polyclonal
- Conjugation:
- Unconjugated
Description
抗体名: | Ripk1 Antibody (PACO52974) |
抗体コード: | PACO52974 |
サイズ: | 50ug |
宿主種: | Rabbit |
申し込み: | ELISA, IP |
推奨される希釈: | ELISA:1:2000-1:10000, IP:1:200-1:2000 |
反応性: | Mouse, Human |
免疫原: | Recombinant Mouse Receptor-interacting serine/threonine-protein kinase 1 protein (1-656AA) |
憲法: | Liquid |
ストレージバッファ: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
精製方法: | >95%, Protein G purified |
抗体のクローン性: | Polyclonal |
アイソタイプ: | IgG |
Conjugate: | Non-conjugated |
Immunoprecipitating Ripk1 in K562 whole cell lysate. Lane 1: Rabbit control IgG (1µg) instead of PACO52974 in K562 whole cell lysate.For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000). Lane 2: PACO52974 (6µg) + K562 whole cell lysate (500µg). Lane 3: K562 whole cell lysate (10µg). |
バックグラウンド: | Serine-threonine kinase which transduces inflammatory and cell-death signals (programmed necrosis) following death receptors ligation, activation of pathogen recognition receptors (PRRs), and DNA damage. Upon activation of TNFR1 by the TNF-α family cytokines, TRADD and TRAF2 are recruited to the receptor. Phosphorylates DAB2IP at 'Ser-728' in a TNF-alpha-dependent manner, and thereby activates the MAP3K5-JNK apoptotic cascade. Ubiquitination by TRAF2 via 'Lys-63'-link chains acts as a critical enhancer of communication with downstream signal transducers in the mitogen-activated protein kinase pathway and the NF-kappa-B pathway, which in turn mediate downstream events including the activation of genes encoding inflammatory molecules. Polyubiquitinated protein binds to IKBKG/NEMO, the regulatory subunit of the IKK complex, a critical event for NF-kappa-B activation. Interaction with other cellular RHIM-containing adapters initiates gene activation and cell death. RIPK1 and RIPK3 association, in particular, forms a necrosis-inducing complex (By similarity). Interacts with ARHGEF2 (By similarity). |
シノニム: | Receptor-interacting serine/threonine-protein kinase 1 (EC 2.7.11.1) (Cell death protein RIP) (Receptor-interacting protein 1) (RIP-1) (Serine/threonine-protein kinase RIP), Ripk1, Rinp Rip |
UniProt Protein Function: | RIPK1: Serine-threonine kinase which transduces inflammatory and cell-death signals (necroptosis) following death receptors ligation, activation of pathogen recognition receptors (PRRs), and DNA damage. Upon activation of TNFR1 by the TNF-alpha family cytokines, TRADD and TRAF2 are recruited to the receptor. Ubiquitination by TRAF2 via 'Lys-63'-link chains acts as a critical enhancer of communication with downstream signal transducers in the mitogen-activated protein kinase pathway and the NF-kappa-B pathway, which in turn mediate downstream events including the activation of genes encoding inflammatory molecules. Polyubiquitinated protein binds to IKBKG/NEMO, the regulatory subunit of the IKK complex, a critical event for NF-kappa-B activation. Interaction with other cellular RHIM-containing adapters initiates gene activation and cell death. RIPK1 and RIPK3 association, in particular, forms a necroptosis-inducing complex. Interacts (via RIP homotypic interaction motif) with RIPK3 (via RIP homotypic interaction motif); this interaction induces RIPK1 necroptosis-specific phosphorylation, formation of the necroptosis-inducing complex. Interacts (via the death domain) with TNFRSF6 (via the death domain) and TRADD (via the death domain). Is recruited by TRADD to TNFRSF1A in a TNF-dependent process. Binds RNF216, EGFR, IKBKG, TRAF1, TRAF2 and TRAF3. Interacts with BNLF1. Interacts with SQSTM1 upon TNF-alpha stimulation. May interact with MAVS/IPS1. Interacts with ZFAND5. Interacts with RBCK1. Interacts with BIRC2/c-IAP1, BIRC3/c-IAP2 and XIAP/BIRC4. Inhibited by necrostatin-1. Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. 2 isoforms of the human protein are produced by alternative splicing. |
UniProt Protein Details: | Protein type:Protein kinase, TKL; Kinase, protein; EC 2.7.11.1; Protein kinase, Ser/Thr (non-receptor); TKL group; RIPK family Cellular Component: protein complex; mitochondrion; membrane; cytoplasm; plasma membrane; receptor complex; lipid raft Molecular Function:transferase activity; identical protein binding; protein serine/threonine kinase activity; protein binding; ubiquitin protein ligase binding; death receptor binding; transferase activity, transferring phosphorus-containing groups; protein complex binding; nucleotide binding; kinase activity; ATP binding; protein kinase activity Biological Process: positive regulation of I-kappaB kinase/NF-kappaB cascade; viral reproduction; positive regulation of apoptosis; protein heterooligomerization; apoptosis; protein amino acid autophosphorylation; programmed cell death; positive regulation of JNK cascade; negative regulation of I-kappaB kinase/NF-kappaB cascade; positive regulation of transferase activity; signal transduction; positive regulation of tumor necrosis factor production; protein amino acid phosphorylation; activation of NF-kappaB transcription factor; positive regulation of interleukin-8 production; positive regulation of programmed cell death; positive regulation of MAPKKK cascade; cellular protein catabolic process; positive regulation of transcription from RNA polymerase II promoter; positive regulation of protein amino acid phosphorylation; positive regulation of macrophage differentiation; phosphorylation; positive regulation of phosphorylation; protein homooligomerization |
UniProt Code: | Q60855 |
NCBI GenInfo Identifier: | 34328467 |
NCBI Gene ID: | 19766 |
NCBI Accession: | NP_033094.3 |
UniProt Secondary Accession: | Q60855,Q3U0J3, Q8CD90, |
UniProt Related Accession: | Q60855 |
Molecular Weight: | 74,854 Da |
NCBI Full Name: | receptor-interacting serine/threonine-protein kinase 1 |
NCBI Synonym Full Names: | receptor (TNFRSF)-interacting serine-threonine kinase 1 |
NCBI Official Symbol: | Ripk1 |
NCBI Official Synonym Symbols: | RIP; Rinp; Rip1; D330015H01Rik |
NCBI Protein Information: | receptor-interacting serine/threonine-protein kinase 1; RIP-1; cell death protein RIP; receptor-interacting protein 1; serine/threonine-protein kinase RIP |
UniProt Protein Name: | Receptor-interacting serine/threonine-protein kinase 1 |
UniProt Synonym Protein Names: | Cell death protein RIP; Receptor-interacting protein 1; RIP-1; Serine/threonine-protein kinase RIP |
Protein Family: | Receptor-interacting serine/threonine-protein kinase |
UniProt Gene Name: | Ripk1 |
UniProt Entry Name: | RIPK1_MOUSE |