PUF60 Antibody (PACO48766)
- SKU:
- PACO48766
- Product type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Application:
- ELISA
- Application:
- WB
- Application:
- IHC
- Antibody type:
- Polyclonal
- Conjugation:
- Unconjugated
Frequently bought together:
Description
抗体名: | PUF60 Antibody (PACO48766) |
抗体コード: | PACO48766 |
サイズ: | 50ug |
宿主種: | Rabbit |
申し込み: | ELISA, WB, IHC |
推奨される希釈: | ELISA:1:2000-1:10000, WB:1:500-1:5000, IHC:1:20-1:200 |
反応性: | Human |
免疫原: | Recombinant Human Poly(U)-binding-splicing factor PUF60 protein (302-413AA) |
憲法: | Liquid |
ストレージバッファ: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
精製方法: | >95%, Protein G purified |
抗体のクローン性: | Polyclonal |
アイソタイプ: | IgG |
Conjugate: | Non-conjugated |
Western Blot. Positive WB detected in: Hela whole cell lysate, MCF-7 whole cell lysate. All lanes: PUF60 antibody at 2.7µg/ml. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 60, 59, 56, 55, 58 kDa. Observed band size: 60 kDa.. | |
Immunohistochemistry of paraffin-embedded human kidney tissue using PACO48766 at dilution of 1:100. |
バックグラウンド: | DNA- and RNA-binding protein, involved in several nuclear processes such as pre-mRNA splicing, apoptosis and transcription regulation. In association with FUBP1 regulates MYC transcription at the P2 promoter through the core-TFIIH basal transcription factor. Acts as a transcriptional repressor through the core-TFIIH basal transcription factor. Represses FUBP1-induced transcriptional activation but not basal transcription. Decreases ERCC3 helicase activity. Does not repress TFIIH-mediated transcription in xeroderma pigmentosum complementation group B (XPB) cells. Is also involved in pre-mRNA splicing. Promotes splicing of an intron with weak 3'-splice site and pyrimidine tract in a cooperative manner with U2AF2. Involved in apoptosis induction when overexpressed in HeLa cells. Isoform 6 failed to repress MYC transcription and inhibited FIR-induced apoptosis in colorectal cancer. Isoform 6 may contribute to tumor progression by enabling increased MYC expression and greater resistance to apoptosis in tumors than in normal cells. Modulates alternative splicing of several mRNAs. Binds to relaxed DNA of active promoter regions. Binds to the pyrimidine tract and 3'-splice site regions of pre-mRNA; binding is enhanced in presence of U2AF2. Binds to Y5 RNA in association with TROVE2. Binds to poly(U) RNA. |
シノニム: | Poly(U)-binding-splicing factor PUF60 (60 kDa poly(U)-binding-splicing factor) (FUSE-binding protein-interacting repressor) (FBP-interacting repressor) (Ro-binding protein 1) (RoBP1) (Siah-binding protein 1) (Siah-BP1), PUF60, FIR ROBPI SIAHBP1 |
UniProt Protein Function: | FIR: fuse-binding protein-interacting repressor. Interacts with (and may activate) Ro RNPs. Forms a ternary complex with far upstream element (FUSE) and FUSE-binding protein. It can repress a c-myc reporter via the FUSE. It is also known to target transcription factor IIH and inhibit activated transcription. This gene is implicated in the xeroderma pigmentosum disorder. Two alternatively spliced isoforms have been described. |
UniProt Protein Details: | Protein type:RNA splicing; RNA-binding; Spliceosome; Transcription, coactivator/corepressor Chromosomal Location of Human Ortholog: 8q24.3 Cellular Component: nucleoplasm; Golgi apparatus; mitochondrion; ribonucleoprotein complex; cyclin-dependent protein kinase activating kinase holoenzyme complex Molecular Function:identical protein binding; protein binding; DNA binding; nucleotide binding Biological Process: transcription, DNA-dependent; regulation of transcription, DNA-dependent; apoptosis; RNA splicing; mRNA processing Disease: Verheij Syndrome |
NCBI Summary: | This gene encodes a nucleic acid-binding protein that plays a role in a variety of nuclear processes, including pre-mRNA splicing and transcriptional regulation. The encoded protein forms a complex with the far upstream DNA element (FUSE) and FUSE-binding protein at the myelocytomatosis oncogene (MYC) promoter. This complex represses MYC transcription through the core-TFIIH basal transcription factor. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Aug 2012] |
UniProt Code: | Q9UHX1 |
NCBI GenInfo Identifier: | 74761960 |
NCBI Gene ID: | 22827 |
NCBI Accession: | Q9UHX1.1 |
UniProt Secondary Accession: | Q9UHX1,Q969E7, Q96D94, Q96H63, Q99628, Q9NZA0, Q9UJY7 A8K8K8, |
UniProt Related Accession: | Q9UHX1 |
Molecular Weight: | 559 |
NCBI Full Name: | Poly(U)-binding-splicing factor PUF60 |
NCBI Synonym Full Names: | poly-U binding splicing factor 60KDa |
NCBI Official Symbol: | PUF60 |
NCBI Official Synonym Symbols: | FIR; VRJS; RoBPI; SIAHBP1 |
NCBI Protein Information: | poly(U)-binding-splicing factor PUF60; poly(U)-binding-splicing factor PUF60; Siah binding protein 1; FBP interacting repressor; Ro ribonucleoprotein-binding protein 1; pyrimidine tract binding splicing factor; FUSE-binding protein-interacting repressor |
UniProt Protein Name: | Poly(U)-binding-splicing factor PUF60 |
UniProt Synonym Protein Names: | 60 kDa poly(U)-binding-splicing factor; FUSE-binding protein-interacting repressor; FBP-interacting repressor; Ro-binding protein 1; RoBP1; Siah-binding protein 1; Siah-BP1 |
Protein Family: | Poly(U)-binding-splicing factor |
UniProt Gene Name: | PUF60 |
UniProt Entry Name: | PUF60_HUMAN |