PPP1R13L Antibody (PACO63475)
- SKU:
- PACO63475
- Product type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Application:
- ELISA
- Application:
- IHC
- Application:
- IF
- Antibody type:
- Polyclonal
- Conjugation:
- Unconjugated
Frequently bought together:
Description
抗体名: | PPP1R13L Antibody (PACO63475) |
抗体コード: | PACO63475 |
サイズ: | 50ul |
宿主種: | Rabbit |
申し込み: | ELISA, IHC, IF |
推奨される希釈: | ELISA:1:2000-1:10000, IHC:1:20-1:200, IF:1:50-1:200 |
反応性: | Human |
免疫原: | Peptide sequence from Human RelA-associated inhibitor protein (83-102AA) |
憲法: | Liquid |
ストレージバッファ: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
精製方法: | Antigen Affinity Purified |
抗体のクローン性: | Polyclonal |
アイソタイプ: | IgG |
Conjugate: | Non-conjugated |
IHC image of PACO63475 diluted at 1:100 and staining in paraffin-embedded human cervical cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. | |
Immunofluorescence staining of A549 cells with PACO63475 at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). |
バックグラウンド: | Regulator that plays a central role in regulation of apoptosis and transcription via its interaction with NF-kappa-B and p53/TP53 proteins. Blocks transcription of HIV-1 virus by inhibiting the action of both NF-kappa-B and SP1. Also inhibits p53/TP53 function, possibly by preventing the association between p53/TP53 and ASPP1 or ASPP2, and therefore suppressing the subsequent activation of apoptosis. |
シノニム: | RelA-associated inhibitor (Inhibitor of ASPP protein) (Protein iASPP) (NFkB-interacting protein 1) (PPP1R13B-like protein), PPP1R13L, IASPP NKIP1 PPP1R13BL RAI |
UniProt Protein Function: | PPP1R13L: an oncoprotein that interacts with and inhibits NF-kappaB, Sp1 and p53. Plays a central role in regulation of apoptosis and transcription. May inhibit p53 by preventing its association with ASPP1 or ASPP2, thereby suppressing the subsequent activation of apoptosis. Required for neuronal survival after axonal injury. Two alternatively spliced isoforms have been described. Isoform 1 is preferrentially found in the cytoplasm; isoform 2 is nuclear. |
UniProt Protein Details: | Protein type:Phosphatase, regulatory subunit; Apoptosis; Transcription, coactivator/corepressor Chromosomal Location of Human Ortholog: 19q13.32 Cellular Component: intercellular bridge; cytoplasm; cell junction; nucleus Molecular Function:identical protein binding; protein binding; transcription corepressor activity; transcription factor binding Biological Process: transcription, DNA-dependent; apoptosis; multicellular organism growth; negative regulation of transcription from RNA polymerase II promoter; multicellular organismal homeostasis; embryonic camera-type eye development; post-embryonic development; cardiac muscle contraction; hair cycle |
NCBI Summary: | IASPP is one of the most evolutionarily conserved inhibitors of p53 (TP53; MIM 191170), whereas ASPP1 (MIM 606455) and ASPP2 (MIM 602143) are activators of p53.[supplied by OMIM, Mar 2008] |
UniProt Code: | Q8WUF5 |
NCBI GenInfo Identifier: | 92090607 |
NCBI Gene ID: | 10848 |
NCBI Accession: | Q8WUF5.4 |
UniProt Secondary Accession: | Q8WUF5,Q5I1X5, |
UniProt Related Accession: | Q8WUF5 |
Molecular Weight: | 828 |
NCBI Full Name: | RelA-associated inhibitor |
NCBI Synonym Full Names: | protein phosphatase 1, regulatory subunit 13 like |
NCBI Official Symbol: | PPP1R13L |
NCBI Official Synonym Symbols: | RAI; RAI4; IASPP; NKIP1 |
NCBI Protein Information: | relA-associated inhibitor; protein iASPP; PPP1R13B-like protein; retinoic acid induced 4; inhibitor of ASPP protein; NFkB interacting protein 1; NFkB-interacting protein 1; inhibitor of apoptosis stimulating protein of p53; protein phosphatase 1, regulato |
UniProt Protein Name: | RelA-associated inhibitor |
UniProt Synonym Protein Names: | Inhibitor of ASPP protein; Protein iASPP; NFkB-interacting protein 1; PPP1R13B-like protein |
UniProt Gene Name: | PPP1R13L |
UniProt Entry Name: | IASPP_HUMAN |