PPARG Antibody (PACO29104)
- SKU:
- PACO29104
- Product type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Application:
- ELISA
- Application:
- IHC
- Application:
- IF
- Antibody type:
- Polyclonal
- Conjugation:
- Unconjugated
Frequently bought together:
Description
抗体名: | PPARG Antibody (PACO29104) |
抗体コード: | PACO29104 |
サイズ: | 50ug |
宿主種: | Rabbit |
申し込み: | ELISA, IHC, IF |
推奨される希釈: | ELISA:1:2000-1:10000, IHC:1:200-1:500, IF:1:50-1:200 |
反応性: | Human |
免疫原: | Recombinant Human Peroxisome proliferator-activated receptor γ protein (1-477AA) |
憲法: | Liquid |
ストレージバッファ: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
精製方法: | >95%, Protein G purified |
抗体のクローン性: | Polyclonal |
アイソタイプ: | IgG |
Conjugate: | Non-conjugated |
IHC image of PACO29104 diluted at 1:200 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. | |
Immunofluorescence staining of PC-3 cells with PACO29104 at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). |
バックグラウンド: | Nuclear receptor that binds peroxisome proliferators such as hypolipidemic drugs and fatty acid, . Once activated by a ligand, the nuclear receptor binds to DNA specific PPAR response elements (PPRE) and modulates the transcription of its target genes, such as acyl-CoA oxidase. It therefore controls the peroxisomal beta-oxidation pathway of fatty acid, . Key regulator of adipocyte differentiation and glucose homeostasis. ARF6 acts as a key regulator of the tissue-specific adipocyte P2 (aP2) enhancer. Acts as a critical regulator of gut homeostasis by suppressing NF-kappa-B-mediated proinflammatory responses. |
シノニム: | Peroxisome proliferator-activated receptor γ (PPAR-gamma) (Nuclear receptor subfamily 1 group C member 3), PPARG, NR1C3 |
UniProt Code: | P37231 |
NCBI GenInfo Identifier: | 545687869 |
NCBI Gene ID: | 102120453 |
NCBI Accession: | NP_001270028.1 |
Molecular Weight: | 59.2kD |
NCBI Full Name: | peroxisome proliferator-activated receptor gamma |
NCBI Official Symbol: | PPARG |
NCBI Official Synonym Symbols: | PPARgamma1; PPARgamma2; PPARgamma3; PPARgamma4; PPARgamma5; PPARgamma6; PPARgamma7 |
NCBI Protein Information: | peroxisome proliferator-activated receptor gamma; hypothetical protein; peroxisome proliferator-activated receptor gamma 1; peroxisome proliferator-activated receptor gamma 2; peroxisome proliferator-activated receptor gamma 3; peroxisome proliferator-activated receptor gamma 4; peroxisome proliferator-activated receptor gamma 5; peroxisome proliferator-activated receptor gamma 6; peroxisome proliferator-activated receptor gamma 7 |
UniProt Protein Name: | |
Protein Family: | Peroxisome proliferator-activated receptor |
UniProt Gene Name: |
Secondary Antibody |
Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014) |
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