Phospho-TOP2A (Ser1106) Antibody (PACO24417)
- SKU:
- PACO24417
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Application:
- ELISA
- Application:
- WB
- Application:
- IHC
- Antibody type:
- Polyclonal
- Conjugation:
- Unconjugated
Frequently bought together:
Description
抗体名: | Phospho-TOP2A (Ser1106) Antibody (PACO24417) |
抗体コード: | PACO24417 |
サイズ: | 100ul |
宿主種: | Rabbit |
申し込み: | ELISA, WB, IHC |
推奨される希釈: | ELISA:1:2000-1:10000, WB:1:500-1:3000, IHC:1:50-1:100 |
反応性: | Human, Mouse |
免疫原: | Peptide sequence around phosphorylation site of serine 1106 (E-E-S(p)-D-N) derived from Human TOP2A. |
憲法: | Liquid |
ストレージバッファ: | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
精製方法: | Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide. |
抗体のクローン性: | Polyclonal |
アイソタイプ: | IgG |
Conjugate: | Non-conjugated |
Western blot analysis of extracts from Jurkat cells, treated with paclitaxel (1uM, 24hours), using TOP2A (Phospho-Ser1106) antibody. The lane on the right is treated with the synthesized peptide. | |
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using TOP2A (Phospho-Ser1106) antibody. The picture on the right is treated with the synthesized peptide. |
バックグラウンド: | Control of topological states of DNA by transient breakage and subsequent rejoining of DNA strands. Topoisomerase II makes double-strand breaks. Essential during mitosis and meiosis for proper segregation of daughter chromosomes. |
シノニム: | α isozyme; DNA topoisomerase II; DNA topoisomerase II-alpha; EC 5.99.1.3; P11388-1; TOP-2; TOP2; TOP2A; TP2A |
UniProt Protein Function: | Control of topological states of DNA by transient breakage and subsequent rejoining of DNA strands. Topoisomerase II makes double-strand breaks. Essential during mitosis and meiosis for proper segregation of daughter chromosomes. May play a role in regulating the period length of ARNTL/BMAL1 transcriptional oscillation. |
NCBI Summary: | This gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This nuclear enzyme is involved in processes such as chromosome condensation, chromatid separation, and the relief of torsional stress that occurs during DNA transcription and replication. It catalyzes the transient breaking and rejoining of two strands of duplex DNA which allows the strands to pass through one another, thus altering the topology of DNA. Two forms of this enzyme exist as likely products of a gene duplication event. The gene encoding this form, alpha, is localized to chromosome 17 and the beta gene is localized to chromosome 3. The gene encoding this enzyme functions as the target for several anticancer agents and a variety of mutations in this gene have been associated with the development of drug resistance. Reduced activity of this enzyme may also play a role in ataxia-telangiectasia. [provided by RefSeq, Jul 2010] |
UniProt Code: | P11388 |
NCBI GenInfo Identifier: | 13959709 |
NCBI Gene ID: | 7153 |
NCBI Accession: | P11388.3 |
UniProt Secondary Accession: | P11388,Q71UN1, Q71UQ5, Q9HB24, Q9HB25, Q9HB26, Q9UP44 Q9UQP9, B2RTS1, |
UniProt Related Accession: | P11388 |
Molecular Weight: | 182,681 Da |
NCBI Full Name: | DNA topoisomerase 2-alpha |
NCBI Synonym Full Names: | topoisomerase (DNA) II alpha |
NCBI Official Symbol: | TOP2A |
NCBI Official Synonym Symbols: | TOP2; TP2A |
NCBI Protein Information: | DNA topoisomerase 2-alpha |
UniProt Protein Name: | DNA topoisomerase 2-alpha |
UniProt Synonym Protein Names: | DNA topoisomerase II, alpha isozyme |
Protein Family: | DNA topoisomerase |
UniProt Gene Name: | TOP2A |
UniProt Entry Name: | TOP2A_HUMAN |
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