PHGDH Antibody (PACO31644)
- SKU:
- PACO31644
- Product type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Application:
- ELISA
- Application:
- WB
- Application:
- IHC
- Application:
- IF
- Antibody type:
- Polyclonal
- Conjugation:
- Unconjugated
Description
抗体名: | PHGDH Antibody (PACO31644) |
抗体コード: | PACO31644 |
サイズ: | 50ug |
宿主種: | Rabbit |
申し込み: | ELISA, WB, IHC, IF |
推奨される希釈: | ELISA:1:2000-1:10000, WB:1:500-1:2000, IHC:1:20-1:200, IF:1:50-1:200 |
反応性: | Human |
免疫原: | Recombinant Human D-3-phosphoglycerate dehydrogenase protein (2-251AA) |
憲法: | Liquid |
ストレージバッファ: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
精製方法: | >95%, Protein G purified |
抗体のクローン性: | Polyclonal |
アイソタイプ: | IgG |
Conjugate: | Non-conjugated |
Western blot All lanes: PHGDH antibody at 2µg/ml Lane 1: EC109 whole cell lysate Lane 2: 293T whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/15000 dilution Predicted band size: 57 kDa Observed band size: 57 kDa . | |
Immunofluorescent analysis of MCF-7 cells using PACO31644 at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). | |
Immunohistochemistry of paraffin-embedded human kidney tissue using PACO31644 at dilution of 1:100. |
バックグラウンド: | Catalyzes the reversible oxidation of 3-phospho-D-glycerate to 3-phosphonooxypyruvate, the first step of the phosphorylated L-serine biosynthesis pathway. Also catalyzes the reversible oxidation of 2-hydroxyglutarate to 2-oxoglutarate and the reversible oxidation of (S)-malate to oxaloacetate. |
シノニム: | D-3-phosphoglycerate dehydrogenase (3-PGDH) (EC 1.1.1.95) (2-oxoglutarate reductase) (EC 1.1.1.399) (Malate dehydrogenase) (EC 1.1.1.37), PHGDH, PGDH3 |
UniProt Protein Function: | PHGDH: Defects in PHGDH are the cause of phosphoglycerate dehydrogenase deficiency (PHGDH deficiency). It is characterized by congenital microcephaly, psychomotor retardation, and seizures. Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family. |
UniProt Protein Details: | Protein type:Oxidoreductase; Amino Acid Metabolism - glycine, serine and threonine; EC 1.1.1.95; Cell development/differentiation Chromosomal Location of Human Ortholog: 1p12 Cellular Component: cytosol Molecular Function:electron carrier activity; NAD binding; phosphoglycerate dehydrogenase activity Biological Process: neural tube development; glial cell development; glycine metabolic process; gamma-aminobutyric acid metabolic process; glutamine metabolic process; L-serine biosynthetic process; spinal cord development; regulation of gene expression; amino acid biosynthetic process; brain development; taurine metabolic process; neurite development; threonine metabolic process Disease: Phosphoglycerate Dehydrogenase Deficiency; Neu-laxova Syndrome 1 |
NCBI Summary: | This gene encodes the enzyme which is involved in the early steps of L-serine synthesis in animal cells. L-serine is required for D-serine and other amino acid synthesis. The enzyme requires NAD/NADH as a cofactor and forms homotetramers for activity. Mutations in this gene have been found in a family with congenital microcephaly, psychomotor retardation and other symptoms. Multiple alternatively spliced transcript variants have been found, however the full-length nature of most are not known. [provided by RefSeq, Aug 2011] |
UniProt Code: | O43175 |
NCBI GenInfo Identifier: | 21264510 |
NCBI Gene ID: | 26227 |
NCBI Accession: | O43175.4 |
UniProt Secondary Accession: | O43175,Q5SZU3, Q9BQ01, B2RD08, |
UniProt Related Accession: | O43175 |
Molecular Weight: | 533 |
NCBI Full Name: | D-3-phosphoglycerate dehydrogenase |
NCBI Synonym Full Names: | phosphoglycerate dehydrogenase |
NCBI Official Symbol: | PHGDH |
NCBI Official Synonym Symbols: | NLS; PDG; PGD; NLS1; PGAD; PGDH; SERA; 3PGDH; 3-PGDH; PHGDHD; HEL-S-113 |
NCBI Protein Information: | D-3-phosphoglycerate dehydrogenase; epididymis secretory protein Li 113 |
UniProt Protein Name: | D-3-phosphoglycerate dehydrogenase |
UniProt Gene Name: | PHGDH |
UniProt Entry Name: | SERA_HUMAN |
Antibodies | ELISA Kits |
Anti-PHGDH Antibody (CAB10461) | Human D-3-phosphoglycerate dehydrogenase (PHGDH) ELISA Kit |
Anti-PHGDH Antibody (CAB19974)[KO Validated] | |
PHGDH Antibody (PACO11214) |
Secondary Antibody |
Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014) |
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