PDCD4 Antibody (PACO43387)
- SKU:
- PACO43387
- Product type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Application:
- ELISA
- Application:
- WB
- Application:
- IP
- Antibody type:
- Polyclonal
- Conjugation:
- Unconjugated
Frequently bought together:
Description
抗体名: | PDCD4 Antibody (PACO43387) |
抗体コード: | PACO43387 |
サイズ: | 50ul |
宿主種: | Rabbit |
申し込み: | ELISA, WB, IP |
推奨される希釈: | ELISA:1:2000-1:10000, WB:1:1000-1:5000, IP:1:200-1:2000 |
反応性: | Human |
免疫原: | Recombinant Human Programmed cell death protein 4 protein (1-260AA) |
憲法: | Liquid |
ストレージバッファ: | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
精製方法: | Antigen Affinity Purified |
抗体のクローン性: | Polyclonal |
アイソタイプ: | IgG |
Conjugate: | Non-conjugated |
Western blot. All lanes: Programmed cell death protein 4 antibody at 5µg/ml. Lane 1: Hela whole cell lysate. Lane 2: MCF-7 whole cell lysate. Lane 3: Jurkat whole cell lysate. Secondary. Goat polyclonal to rabbit IgG at 1/10000 dilution. Predicted band size: 52, 51 kDa. Observed band size: 52 kDa. | |
Immunoprecipitating PDCD4 in Hela whole cell lysate. Lane 1: Rabbit control IgG instead of (1µg) instead of PACO43387 in Hela whole cell lysate.For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the Secondary antibody (1/50000). Lane 2: PACO43387 (4µg) + Hela whole cell lysate (500µg). Lane 3: Hela whole cell lysate (20µg). |
バックグラウンド: | Inhibits translation initiation and cap-dependent translation. May excert its function by hindering the interaction between EIF4A1 and EIF4G. Inhibits the helicase activity of EIF4A. Modulates the activation of JUN kinase. Down-regulates the expression of MAP4K1, thus inhibiting events important in driving invasion, namely, MAPK85 activation and consequent JUN-dependent transcription. May play a role in apoptosis. Tumor suppressor. Inhibits tumor promoter-induced neoplastic transformation. Binds RNA. |
シノニム: | Programmed cell death protein 4 (Neoplastic transformation inhibitor protein) (Nuclear antigen H731-like) (Protein 197/15a), PDCD4, H731 |
UniProt Protein Function: | PDCD4: protein localized to the nucleus in proliferating cells that seems to possess a tumor suppressor activity. Directly interacts with the RNA helicase eIF4A and inhibits protein synthesis by interfering with the assembly of the cap-dependent translation initiation complex. Suppresses carbonic anhydrase type II protein expression in carcinoid cell lines. Since tumor cells require a high bicarbonate flux for their growth, carbonic anhydrase suppression results in growth inhibition. Expression of this gene is modulated by cytokines in natural killer and T cells. The gene product is thought to play a role in apoptosis but the specific role has not yet been determined. Two differentially spliced isoforms have been identified. |
UniProt Protein Details: | Protein type:Tumor suppressor; Apoptosis Chromosomal Location of Human Ortholog: 10q24 Cellular Component: cytoplasm; cytosol; nucleoplasm; nucleus Molecular Function:protein binding; RNA binding Biological Process: apoptosis; cell aging; negative regulation of apoptosis; negative regulation of cell cycle; negative regulation of JNK activity; negative regulation of transcription, DNA-dependent |
NCBI Summary: | This gene is a tumor suppressor and encodes a protein that binds to the eukaryotic translation initiation factor 4A1 and inhibits its function by preventing RNA binding. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2010] |
UniProt Code: | Q53EL6 |
NCBI GenInfo Identifier: | 317373317 |
NCBI Gene ID: | 27250 |
NCBI Accession: | Q53EL6.2 |
UniProt Secondary Accession: | Q53EL6,O15501, Q5VZS6, Q6PJI5, Q8TAR5, Q99834, B2RCV4 B5ME91, |
UniProt Related Accession: | Q53EL6 |
Molecular Weight: | 50,576 Da |
NCBI Full Name: | Programmed cell death protein 4 |
NCBI Synonym Full Names: | programmed cell death 4 (neoplastic transformation inhibitor) |
NCBI Official Symbol: | PDCD4 |
NCBI Official Synonym Symbols: | H731 |
NCBI Protein Information: | programmed cell death protein 4 |
UniProt Protein Name: | Programmed cell death protein 4 |
UniProt Synonym Protein Names: | Neoplastic transformation inhibitor protein; Nuclear antigen H731-like; Protein 197/15a |
Protein Family: | Programmed cell death protein |
UniProt Gene Name: | PDCD4 |
UniProt Entry Name: | PDCD4_HUMAN |
Secondary Antibody |
Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014) |
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Anti-FITC Goat Anti-Rabbit IgG (H+L) Antibody (CABS011) |
Anti-HRP-conjugated Beta Actin Antibody (CABC028) |