PAFAH1B3 Antibody (PACO57132)
- SKU:
- PACO57132
- Product type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Application:
- ELISA
- Application:
- IHC
- Application:
- IF
- Antibody type:
- Polyclonal
- Conjugation:
- Unconjugated
Frequently bought together:
Description
抗体名: | PAFAH1B3 Antibody (PACO57132) |
抗体コード: | PACO57132 |
サイズ: | 50ug |
宿主種: | Rabbit |
申し込み: | ELISA, IHC, IF |
推奨される希釈: | ELISA:1:2000-1:10000, IHC:1:500-1:1000, IF:1:200-1:500 |
反応性: | Human |
免疫原: | Recombinant Human Platelet-activating factor acetylhydrolase IB subunit γ protein (103-187AA) |
憲法: | Liquid |
ストレージバッファ: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
精製方法: | >95%, Protein G purified |
抗体のクローン性: | Polyclonal |
アイソタイプ: | IgG |
Conjugate: | Non-conjugated |
IHC image of PACO57132 diluted at 1:600 and staining in paraffin-embedded human liver tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. | |
Immunofluorescence staining of A549 cells with PACO57132 at 1:200, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). | |
IHC image of PACO57132 diluted at 1:600 and staining in paraffin-embedded human pancreatic cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
バックグラウンド: | Inactivates paf by removing the acetyl group at the sn-2 position. This is a catalytic subunit. Plays an important role during the development of brain. |
シノニム: | Platelet-activating factor acetylhydrolase IB subunit γ (EC 3.1.1.47) (PAF acetylhydrolase 29 kDa subunit) (PAF-AH 29 kDa subunit) (PAF-AH subunit gamma) (PAFAH subunit gamma), PAFAH1B3, PAFAHG |
UniProt Code: | Q15102 |
NCBI GenInfo Identifier: | 1122219 |
NCBI Gene ID: | 5050 |
NCBI Accession: | BAA09706.1 |
UniProt Secondary Accession: | Q15102,Q53X88, |
UniProt Related Accession: | Q15102 |
Molecular Weight: | 25,734 Da |
NCBI Full Name: | platelet activating factor acetylhydrolase IB gamma-subunit |
NCBI Synonym Full Names: | platelet-activating factor acetylhydrolase 1b, catalytic subunit 3 (29kDa) |
NCBI Official Symbol: | PAFAH1B3 |
NCBI Official Synonym Symbols: | PAFAHG |
NCBI Protein Information: | platelet-activating factor acetylhydrolase IB subunit gamma; PAFAH subunit gamma; PAF-AH subunit gamma; PAF-AH 29 kDa subunit; PAF-AH1b alpha 1 subunit; PAF acetylhydrolase 29 kDa subunit; platelet-activating factor acetylhydrolase, isoform Ib, subunit 3 (29kDa) |
UniProt Protein Name: | Platelet-activating factor acetylhydrolase IB subunit gamma |
UniProt Synonym Protein Names: | PAF acetylhydrolase 29 kDa subunit; PAF-AH 29 kDa subunit; PAF-AH subunit gamma |
Protein Family: | PCNA-associated factor |
UniProt Gene Name: | PAFAH1B3 |
UniProt Entry Name: | PA1B3_HUMAN |
Antibodies | ELISA Kits |
Anti-PAFAH1B3 Antibody (CAB13037) | Human Platelet-activating factor acetylhydrolase IB subunit gamma (PAFAH1B3) ELISA Kit |
PAFAH1B3 Antibody (PACO11052) |
Secondary Antibody |
Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014) |
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Anti-HRP-conjugated Beta Actin Antibody (CABC028) |