NFKBIA Antibody (PACO64339)
- SKU:
- PACO64339
- Product type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Application:
- ELISA
- Application:
- WB
- Application:
- IHC
- Application:
- IF
- Antibody type:
- Polyclonal
- Conjugation:
- Unconjugated
Frequently bought together:
Description
抗体名: | NFKBIA Antibody (PACO64339) |
抗体コード: | PACO64339 |
サイズ: | 50ul |
宿主種: | Rabbit |
申し込み: | ELISA, WB, IHC, IF |
推奨される希釈: | WB:1:1000-1:5000, IHC:1:100-1:500, IF:1:100-1:500 |
反応性: | Human |
免疫原: | Recombinant Human NF-kappa-B inhibitor α protein (27-317AA) |
憲法: | Liquid |
ストレージバッファ: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
精製方法: | >95%, Antigen Affinity purified & Affinity purified |
抗体のクローン性: | Polyclonal |
アイソタイプ: | IgG |
Conjugate: | Non-conjugated |
Western Blot. Positive WB detected in: Jurkat whole cell lysate, Hela whole cell lysate, Raji whole cell lysate. All lanes: NFKBIA antibody at 1:2000. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 36 kDa. Observed band size: 36 kDa. | |
Immunofluorescence staining of HepG2 cells with PACO64339 at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). | |
IHC image of PACO64339 diluted at 1:100 and staining in paraffin-embedded human adrenal gland tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. |
バックグラウンド: | Inhibits the activity of dimeric NF-kappa-B/REL complexes by trapping REL dimers in the cytoplasm through masking of their nuclear localization signals. On cellular stimulation by immune and proinflammatory responses, becomes phosphorylated promoting ubiquitination and degradation, enabling the dimeric RELA to translocate to the nucleus and activate transcription. |
シノニム: | NF-kappa-B inhibitor α (I-kappa-B-alpha) (IkB-alpha) (IkappaBalpha) (Major histocompatibility complex enhancer-binding protein MAD3), NFKBIA, IKBA MAD3 NFKBI |
UniProt Protein Function: | Inhibits the activity of dimeric NF-kappa-B/REL complexes by trapping REL dimers in the cytoplasm through masking of their nuclear localization signals. On cellular stimulation by immune and proinflammatory responses, becomes phosphorylated promoting ubiquitination and degradation, enabling the dimeric RELA to translocate to the nucleus and activate transcription. |
NCBI Summary: | This gene encodes a member of the NF-kappa-B inhibitor family, which contain multiple ankrin repeat domains. The encoded protein interacts with REL dimers to inhibit NF-kappa-B/REL complexes which are involved in inflammatory responses. The encoded protein moves between the cytoplasm and the nucleus via a nuclear localization signal and CRM1-mediated nuclear export. Mutations in this gene have been found in ectodermal dysplasia anhidrotic with T-cell immunodeficiency autosomal dominant disease. [provided by RefSeq, Aug 2011] |
UniProt Code: | P25963 |
NCBI GenInfo Identifier: | 126682 |
NCBI Gene ID: | 4792 |
NCBI Accession: | P25963.1 |
UniProt Secondary Accession: | P25963,B2R8L6, |
UniProt Related Accession: | P25963 |
Molecular Weight: | 35,609 Da |
NCBI Full Name: | NF-kappa-B inhibitor alpha |
NCBI Synonym Full Names: | NFKB inhibitor alpha |
NCBI Official Symbol: | NFKBIA |
NCBI Official Synonym Symbols: | IKBA; MAD-3; NFKBI |
NCBI Protein Information: | NF-kappa-B inhibitor alpha |
UniProt Protein Name: | NF-kappa-B inhibitor alpha |
UniProt Synonym Protein Names: | I-kappa-B-alpha; IkB-alpha; IkappaBalpha; Major histocompatibility complex enhancer-binding protein MAD3 |
Protein Family: | NF-kappa-B inhibitor |
UniProt Gene Name: | NFKBIA |
UniProt Entry Name: | IKBA_HUMAN |
Secondary Antibody |
Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014) |
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