NEFL Antibody (PACO46066)
- SKU:
- PACO46066
- Product type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Application:
- ELISA
- Application:
- IHC
- Application:
- IF
- Application:
- IP
- Antibody type:
- Polyclonal
- Conjugation:
- Unconjugated
Description
抗体名: | NEFL Antibody (PACO46066) |
抗体コード: | PACO46066 |
サイズ: | 50ug |
宿主種: | Rabbit |
申し込み: | ELISA, IHC, IF, IP |
推奨される希釈: | ELISA:1:2000-1:10000, IHC:1:20-1:200, IF:1:50-1:200, IP:1:200-1:2000 |
反応性: | Human |
免疫原: | Recombinant Human Neurofilament light polypeptide protein (128-271AA) |
憲法: | Liquid |
ストレージバッファ: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
精製方法: | >95%, Protein G purified |
抗体のクローン性: | Polyclonal |
アイソタイプ: | IgG |
Conjugate: | Non-conjugated |
Immunohistochemistry of paraffin-embedded human brain tissue using PACO46066 at dilution of 1:100. | |
Immunoprecipitating NEFL in mouse brain tissue. Lane 1: Rabbit control IgG instead of PACO46066 in mouse brain tissue.For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000). Lane 2: PACO46066 (8µg) + Mouse brain tissue (500µg). Lane 3: Mouse brain tissue (10µg). | |
Immunofluorescent analysis of SH-SY5Y cells using PACO46066 at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). |
バックグラウンド: | Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber. |
シノニム: | Neurofilament light polypeptide (NF-L) (68 kDa neurofilament protein) (Neurofilament triplet L protein), NEFL, NF68 NFL |
UniProt Protein Function: | NFL: one of the three (L, M, and H) intermediate filament proteins that form neurofilaments. Neurofilaments are involved in the maintenance of neuronal caliber. NF-L is the most abundant of the three neurofilament proteins. Defects cause Charcot-Marie-Tooth disease type 1F (CMT1F). |
UniProt Protein Details: | Protein type:Cytoskeletal Chromosomal Location of Human Ortholog: 8p21 Cellular Component: TSC1-TSC2 complex; growth cone; axon; cytoplasm; neurofilament; cytosol Molecular Function:protein C-terminus binding; protein binding, bridging; identical protein binding; protein domain specific binding; protein binding; phospholipase binding; structural constituent of cytoskeleton Biological Process: protein polymerization; response to peptide hormone stimulus; neurofilament bundle assembly; response to toxin; intermediate filament organization; microtubule cytoskeleton organization and biogenesis; retrograde axon cargo transport; axon regeneration in the peripheral nervous system; synaptic transmission; axon transport of mitochondrion; regulation of axon diameter; positive regulation of axonogenesis; negative regulation of neuron apoptosis; anterograde axon cargo transport; neuromuscular process controlling balance; locomotion; neurite morphogenesis; response to corticosterone stimulus Disease: Charcot-marie-tooth Disease, Axonal, Type 2e; Charcot-marie-tooth Disease, Demyelinating, Type 1f |
NCBI Summary: | Neurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and they functionally maintain the neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. This gene encodes the light chain neurofilament protein. Mutations in this gene cause Charcot-Marie-Tooth disease types 1F (CMT1F) and 2E (CMT2E), disorders of the peripheral nervous system that are characterized by distinct neuropathies. A pseudogene has been identified on chromosome Y. [provided by RefSeq, Oct 2008] |
UniProt Code: | P07196 |
NCBI GenInfo Identifier: | 62511894 |
NCBI Gene ID: | 4747 |
NCBI Accession: | P07196.3 |
UniProt Related Accession: | P07196 |
Molecular Weight: | |
NCBI Full Name: | Neurofilament light polypeptide |
NCBI Synonym Full Names: | neurofilament light |
NCBI Official Symbol: | NEFL |
NCBI Official Synonym Symbols: | NFL; NF-L; NF68; CMT1F; CMT2E; CMTDIG; PPP1R110 |
NCBI Protein Information: | neurofilament light polypeptide |
UniProt Protein Name: | Neurofilament light polypeptide |
UniProt Synonym Protein Names: | 68 kDa neurofilament protein; Neurofilament triplet L protein |
Protein Family: | Neurofilament heavy polypeptide |
UniProt Gene Name: | NEFL |
UniProt Entry Name: | NFL_HUMAN |
Secondary Antibody |
Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014) |
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