MLH1 Antibody, HRP conjugated (PACO46411)
- SKU:
- PACO46411
- Product type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Application:
- ELISA
- Antibody type:
- Polyclonal
- Conjugation:
- HRP
Frequently bought together:
Description
抗体名: | MLH1 Antibody, HRP conjugated (PACO46411) |
抗体コード: | PACO46411 |
サイズ: | 50ug |
宿主種: | Rabbit |
申し込み: | ELISA |
推奨される希釈: | |
反応性: | Human |
免疫原: | Recombinant Human DNA mismatch repair protein Mlh1 protein (350-519AA) |
憲法: | Liquid |
ストレージバッファ: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
精製方法: | >95%, Protein G purified |
抗体のクローン性: | Polyclonal |
アイソタイプ: | IgG |
Conjugate: | HRP |
バックグラウンド: | Heterodimerizes with PMS2 to form MutL alpha, a component of the post-replicative DNA mismatch repair system (MMR). DNA repair is initiated by MutS α (MSH2-MSH6) or MutS β (MSH2-MSH6) binding to a dsDNA mismatch, then MutL α is recruited to the heteroduplex. Assembly of the MutL-MutS-heteroduplex ternary complex in presence of RFC and PCNA is sufficient to activate endonuclease activity of PMS2. It introduces single-strand breaks near the mismatch and thus generates new entry points for the exonuclease EXO1 to degrade the strand containing the mismatch. DNA methylation would prevent cleavage and therefore assure that only the newly mutated DNA strand is going to be corrected. MutL α (MLH1-PMS2) interacts physically with the clamp loader subunits of DNA polymerase III, suggesting that it may play a role to recruit the DNA polymerase III to the site of the MMR. Also implicated in DNA damage signaling, a process which induces cell cycle arrest and can lead to apoptosis in case of major DNA damages. Heterodimerizes with MLH3 to form MutL γ which plays a role in meiosis. |
シノニム: | DNA mismatch repair protein Mlh1 (MutL protein homolog 1), MLH1, COCA2 |
UniProt Protein Function: | MLH1: a protein involved in the repair of mismatches in DNA. Binds PMS2 or MLH1 and MLH3. Part of the BRCA1-associated genome surveillance complex (BASC), which contains BRCA1, MSH2, MSH6, MLH1, ATM, BLM, PMS2 and the RAD50- MRE11-NBS1 protein complex. This association could be a dynamic process changing throughout the cell cycle and within subnuclear domains. Interacts with MBD4. Interacts with EXO1. Defects in MLH1 are the cause of hereditary non-polyposis colorectal cancer type 2; Turcot syndrome, an autosomal dominant disorder characterized by malignant tumors of the brain; Muir-Torre syndrome (MTS), a rare autosomal dominant disorder characterized by sebaceous neoplasms and visceral malignancy; lobular carcinoma in situ; endometrial cancer; and non-polyposis colorectal cancer. |
UniProt Protein Details: | Protein type:Tumor suppressor; DNA repair, damage Chromosomal Location of Human Ortholog: 3p21.3 Cellular Component: chiasma; membrane; MutLalpha complex; nucleoplasm; nucleus; synaptonemal complex Molecular Function:ATPase activity; MutSalpha complex binding; protein binding; single-stranded DNA binding Biological Process: mismatch repair; somatic hypermutation of immunoglobulin genes Disease: Colorectal Cancer, Hereditary Nonpolyposis, Type 2; Mismatch Repair Cancer Syndrome; Muir-torre Syndrome |
NCBI Summary: | This gene was identified as a locus frequently mutated in hereditary nonpolyposis colon cancer (HNPCC). It is a human homolog of the E. coli DNA mismatch repair gene mutL, consistent with the characteristic alterations in microsatellite sequences (RER+phenotype) found in HNPCC. Alternative splicing results in multiple transcript variants encoding distinct isoforms. Additional transcript variants have been described, but their full-length natures have not been determined.[provided by RefSeq, Nov 2009] |
UniProt Code: | P40692 |
NCBI GenInfo Identifier: | 730028 |
NCBI Gene ID: | 4292 |
NCBI Accession: | P40692.1 |
UniProt Secondary Accession: | P40692,B4DI13, B4DQ11, E9PCU2, |
UniProt Related Accession: | P40692 |
Molecular Weight: | 73,837 Da |
NCBI Full Name: | DNA mismatch repair protein Mlh1 |
NCBI Synonym Full Names: | mutL homolog 1 |
NCBI Official Symbol: | MLH1 |
NCBI Official Synonym Symbols: | FCC2; COCA2; HNPCC; hMLH1; HNPCC2 |
NCBI Protein Information: | DNA mismatch repair protein Mlh1 |
UniProt Protein Name: | DNA mismatch repair protein Mlh1 |
UniProt Synonym Protein Names: | MutL protein homolog 1 |
Protein Family: | Putative MutL protein |
UniProt Gene Name: | MLH1 |
UniProt Entry Name: | MLH1_HUMAN |