MASP2 Antibody (PACO55618)
- SKU:
- PACO55618
- Product type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Application:
- ELISA
- Application:
- WB
- Application:
- IHC
- Antibody type:
- Polyclonal
- Conjugation:
- Unconjugated
Frequently bought together:
Description
抗体名: | MASP2 Antibody (PACO55618) |
抗体コード: | PACO55618 |
サイズ: | 50ug |
宿主種: | Rabbit |
申し込み: | ELISA, WB, IHC |
推奨される希釈: | ELISA:1:2000-1:10000, WB:1:500-1:5000, IHC:1:20-1:200 |
反応性: | Human |
免疫原: | Recombinant Human Mannan-binding lectin serine protease 2 protein (189-314AA) |
憲法: | Liquid |
ストレージバッファ: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
精製方法: | >95%, Protein G purified |
抗体のクローン性: | Polyclonal |
アイソタイプ: | IgG |
Conjugate: | Non-conjugated |
Western Blot. Positive WB detected in: A549 whole cell lysate, SH-SY5Y whole cell lysate. All lanes: MASP2 antibody at 2.7µg/ml. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 76, 21 kDa. Observed band size: 76 kDa. | |
IHC image of PACO55618 diluted at 1:100 and staining in paraffin-embedded human melanoma performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
バックグラウンド: | Serum protease that plays an important role in the activation of the complement system via mannose-binding lectin. After activation by auto-catalytic cleavage it cleaves C2 and C4, leading to their activation and to the formation of C3 convertase. |
シノニム: | Mannan-binding lectin serine protease 2 (EC 3.4.21.104) (MBL-associated serine protease 2) (Mannose-binding protein-associated serine protease 2) (MASP-2) [Cleaved into: Mannan-binding lectin serine protease 2 A chain; Mannan-binding lectin serine protease 2 B chain], MASP2 |
UniProt Protein Function: | MASP2: Serum protease that plays an important role in the activation of the complement system via mannose-binding lectin. After activation by auto-catalytic cleavage it cleaves C2 and C4, leading to their activation and to the formation of C3 convertase. Defects in MASP2 are the cause of MASP2 deficiency (MASPD). MASPD is a disorder that results in autoimmune manifestations, recurrent severe infections, and chronic inflammatory disease. Belongs to the peptidase S1 family. 2 isoforms of the human protein are produced by alternative splicing. |
UniProt Protein Details: | Protein type:EC 3.4.21.104; Protease; Secreted, signal peptide; Secreted Chromosomal Location of Human Ortholog: 1p36.3-p36.2 Cellular Component: extracellular region Molecular Function:peptidase activity; protein binding; serine-type endopeptidase activity; complement component C4b binding; calcium ion binding; calcium-dependent protein binding Biological Process: innate immune response; complement activation, lectin pathway; proteolysis; complement activation, classical pathway; complement activation Disease: Masp2 Deficiency |
NCBI Summary: | The Ra-reactive factor (RARF) is a complement-dependent bactericidal factor that binds to the Ra and R2 polysaccharides expressed by certain enterobacteria. Alternate splicing of this gene results in two transcript variants encoding two RARF components that are involved in the mannan-binding lectin pathway of complement activation. The longer isoform is cleaved into two chains which form a heterodimer linked by a disulfide bond. The encoded proteins are members of the trypsin family of peptidases. [provided by RefSeq, Jul 2008] |
UniProt Code: | O00187 |
NCBI GenInfo Identifier: | 21264363 |
NCBI Gene ID: | 10747 |
NCBI Accession: | NP_006601.2 |
UniProt Secondary Accession: | O00187,O75754, Q5TEQ5, Q5TER0, Q96QG4, Q9BZH0, Q9H498 Q9H499, Q9UBP3, Q9UC48, A8K458, A8MWJ2, |
UniProt Related Accession: | O00187 |
Molecular Weight: | 76 kDa |
NCBI Full Name: | mannan-binding lectin serine protease 2 isoform 1 preproprotein |
NCBI Synonym Full Names: | mannan-binding lectin serine peptidase 2 |
NCBI Official Symbol: | MASP2 |
NCBI Official Synonym Symbols: | sMAP; MAP19; MASP-2; MASP1P1 |
NCBI Protein Information: | mannan-binding lectin serine protease 2 |
UniProt Protein Name: | Mannan-binding lectin serine protease 2 |
UniProt Synonym Protein Names: | MBL-associated serine protease 2; Mannose-binding protein-associated serine protease 2; MASP-2 |
Protein Family: | Mannan-binding lectin serine protease |
UniProt Gene Name: | MASP2 |
UniProt Entry Name: | MASP2_HUMAN |
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