IDH3A Antibody (PACO53290)
- SKU:
- PACO53290
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Application:
- ELISA
- Application:
- WB
- Application:
- IHC
- Application:
- IF
- Application:
- IP
- Antibody type:
- Polyclonal
- Conjugation:
- Unconjugated
Frequently bought together:
Description
抗体名: | IDH3A Antibody (PACO53290) |
抗体コード: | PACO53290 |
サイズ: | 50ug |
宿主種: | Rabbit |
申し込み: | ELISA, WB, IHC, IF, IP |
推奨される希釈: | ELISA:1:2000-1:10000, WB:1:500-1:5000, IHC:1:20-1:200, IF:1:50-1:200, IP:1:200-1:2000, |
反応性: | Human, Mouse, Rat |
免疫原: | Recombinant Human Isocitrate dehydrogenase [NAD] subunit alpha, mitochondrial protein (28-108AA) |
憲法: | Liquid |
ストレージバッファ: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
精製方法: | >95%, Protein G purified |
抗体のクローン性: | Polyclonal |
アイソタイプ: | IgG |
Conjugate: | Non-conjugated |
Western Blot. Positive WB detected in: HepG2 whole cell lysat, Jurkat whole cell lysate, Rat brain tissue, Mouse brain tissue, Mouse liver tissue. All lanes: IDH3A antibody at 3.4µg/ml. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 40, 32 kDa. Observed band size: 40 kDa. | |
Immunofluorescent analysis of A549 cells using PACO53290 at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). | |
Immunohistochemistry of paraffin-embedded human adrenal gland tissue using PACO53290 at dilution of 1:100. |
バックグラウンド: | mitochondrial matrix, mitochondrion, nucleus, carbohydrate metabolic process, tricarboxylic acid, cycle |
シノニム: | Isocitrate dehydrogenase [NAD] subunit alpha, mitochondrial (EC 1.1.1.41) (Isocitric dehydrogenase subunit alpha) (NAD(+)-specific ICDH subunit alpha), IDH3A |
UniProt Protein Function: | IDH3A: a enzyme that that catalyzes the oxidative decarboxylation of isocitrate to alpha-ketoglutarate (alpha-KG), requiring nicotinamide adenine dinucleotide (NAD) as a cofactor. The holoenzyme is a heterooligomer of subunits alpha, beta, and gamma in the apparent ratio of 2:1:1. IDH3 is a mitochondrial enzyme that functions in the Krebs cycle, which generates substrates for cellular respiration. Alpha-KG can exit the mitochondria and enter other cellular compartments where it is a cofactor for the dioxygenases that hydroxylate the transcription factor HIF and trigger its degradation by VHL. Since HIF turns on oncogenic pathways, IDH3 has apparent tumor suppressor activity. Two isoforms of the human protein are produced by alternative splicing. |
UniProt Protein Details: | Protein type:Mitochondrial; EC 1.1.1.41; Oxidoreductase; Carbohydrate Metabolism - citrate (TCA) cycle Chromosomal Location of Human Ortholog: 15q25.1-q25.2 Cellular Component: mitochondrial matrix; mitochondrion; nucleus Biological Process: tricarboxylic acid cycle |
NCBI Summary: | Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. NAD(+)-dependent isocitrate dehydrogenases catalyze the allosterically regulated rate-limiting step of the tricarboxylic acid cycle. Each isozyme is a heterotetramer that is composed of two alpha subunits, one beta subunit, and one gamma subunit. The protein encoded by this gene is the alpha subunit of one isozyme of NAD(+)-dependent isocitrate dehydrogenase. [provided by RefSeq, Jul 2008] |
UniProt Code: | P50213 |
NCBI GenInfo Identifier: | 1708399 |
NCBI Gene ID: | 3419 |
NCBI Accession: | P50213.1 |
UniProt Secondary Accession: | P50213,Q9H3X0, D3DW83, |
UniProt Related Accession: | P50213 |
Molecular Weight: | 31,381 Da |
NCBI Full Name: | Isocitrate dehydrogenase |
NCBI Synonym Full Names: | isocitrate dehydrogenase 3 (NAD(+)) alpha |
NCBI Official Symbol: | IDH3A |
NCBI Protein Information: | isocitrate dehydrogenase [NAD] subunit alpha, mitochondrial |
UniProt Protein Name: | Isocitrate dehydrogenase [NAD] subunit alpha, mitochondrial |
UniProt Synonym Protein Names: | Isocitric dehydrogenase subunit alpha; NAD(+)-specific ICDH subunit alpha |
Protein Family: | Isocitrate dehydrogenase |
UniProt Gene Name: | IDH3A |
UniProt Entry Name: | IDH3A_HUMAN |